Relationship between apoptosis and thymocyte depletion in rabies-infected mice.
Braz J Med Biol Res
; 27(7): 1599-603, 1994 Jul.
Article
em En
| MEDLINE
| ID: mdl-7874026
ABSTRACT
The apoptosis of thymocytes from rabies-infected mice was investigated in a kinetic study covering the entire course of the infection. For this study, BALB/c mice (6-7-week old females) were inoculated intracerebrally with 100 LD50 of Challenge Virus Strain, a fixed rabies virus strain, and three animals were sacrificed per time point to remove thymuses. When thymocytes were fixed, stained with propidium iodide and analyzed by flow cytometry, a distinct subpopulation of cells was observed below the G0/G1 region, denoted as the A0 region. Cells in this region presented reduced fluorescence, and nuclear DNA fragmentation. The accumulation of cells in the A0 region, after infection, progressively increased, reaching 12% for unfractionated thymocytes, 62% for thymocytes from the 60% Percoll interface and 32% for thymocytes recovered at the 100% Percoll interface. This finding, observed only in thymocytes from infected mice, demonstrates a clear modification of chromatin condensation in these cells, suggesting the occurrence of an apoptotic process during rabies infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raiva
/
Timo
/
Linfócitos T
/
Depleção Linfocítica
/
Apoptose
/
Camundongos Endogâmicos BALB C
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Brasil