Effect of follicular fluid supplementation on the in vitro development of human pre-embryos.
Fertil Steril
; 62(5): 1018-21, 1994 Nov.
Article
em En
| MEDLINE
| ID: mdl-7926112
ABSTRACT
OBJECTIVE:
To evaluate the value of supplementing a standard culture medium with 10% heat-inactivated mature follicular fluid (FF).DESIGN:
Prospective randomized study evaluating the in vitro development of nontransferred, nonfrozen human pre-embryos in three culture conditions from day 3 to day 8 postfertilization. Preliminary evaluation by RIA and electrophoresis of factors responsible for these results.RESULTS:
Ten percent mature FF supplementation of Inra Menezo (B2 medium) was associated with a significantly higher proportion of human pre-embryos reaching the morula (95% versus 72% with 10% maternal serum, P = 0.04) and the blastocyst stage (50% versus 11% with B2 alone, P = 0.02). The concentration of insulin-like growth factors I and II did not differ significantly between serum and mature FF-supplemented medium. Protein electrophoresis showed a difference of two bands corresponding to a molecular weight of 17,000 present in the serum and not in the FF-supplemented medium.CONCLUSION:
Culture medium supplementation with 10% mature FF is associated with a significantly higher proportion of pre-embryos reaching the morula and blastocyst stage. The observed difference could be explained by the presence of a low molecular weight (17,000) embryotoxic factor contained in the serum-supplemented medium.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zigoto
/
Líquido Folicular
/
Meios de Cultura
Tipo de estudo:
Clinical_trials
/
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Canadá