Stoichiometry of the complex of human interleukin-4 with its receptor.
FEBS Lett
; 347(1): 17-21, 1994 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-8013654
ABSTRACT
A large number of cytokines have been shown to possess a four-helix bundle structure with a unique up-up-down-down connectivity. The receptors for this family of cytokines have been shown to be homologous as well, each possessing two tandem repeats of a fibronectin type III-like domain. The crystal structure of human growth hormone bound to the soluble portion of its receptor has served as the only experimentally-determined example of the interaction between the four-helix bundle cytokines and their receptors two identical receptor subunits bind to different epitopes on the same growth hormone ligand. We have conducted a series of experiments to determine if this structural paradigm is true for interleukin-4 and interleukin-4 receptor. Native polyacrylamide gel electrophoresis and gel filtration chromatography reveal that interleukin-4 forms a tight 11 complex with the system.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Mitogênicos
/
Interleucina-4
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1994
Tipo de documento:
Article