Crosslinked CNBr-activated hyaluronan-collagen matrices: effects on fibroblast contraction.
Matrix Biol
; 14(2): 147-57, 1994 Mar.
Article
em En
| MEDLINE
| ID: mdl-8061927
ABSTRACT
Previously we demonstrated that the contraction of collagen fibrillar matrices (CFM) by fibroblasts was significantly reduced when high concentrations (> 1 mg/ml) of hyaluronan were present in the media. This highlights the prominent role of hyaluronan in preventing scar formation during fetal wound healing. In the present study, we covalently crosslinked different concentrations of CNBr-activated hyaluronan to CFM and investigated the ability of fibroblasts to contract these matrices. Matrices are not contracted when optimal concentrations of CNBr-activated hyaluronan are crosslinked to collagen. Biochemical and cytological analysis of these matrices suggests that hyaluronan strengthens the collagen fibrils and blocks direct communication between fibroblasts and the collagen fibrils. Based on current evidence we are able to propose two molecular crosslinking models, multiple point attachment and simple point attachment between CNBr-activated hyaluronan and collagen molecules, hypotheses supported by the susceptibility of these matrices to digestion by collagenase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colágeno
/
Brometo de Cianogênio
/
Matriz Extracelular
/
Fibroblastos
/
Ácido Hialurônico
Tipo de estudo:
Prognostic_studies
Limite:
Humans
/
Male
Idioma:
En
Revista:
Matrix Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1994
Tipo de documento:
Article