Effect of tumour promoting agents on protein phosphorylation in human placenta.
Int J Biochem
; 26(7): 923-31, 1994 Jul.
Article
em En
| MEDLINE
| ID: mdl-8063016
ABSTRACT
1. The effects of okadaic acid (OA) and phorbol-12-myristate-13-acetate (PMA) on protein phosphorylation were studied in human term placentas. 2. When samples treated with tumour promoters were compared with untreated samples, the phosphorylation of a 135 kDa protein was significantly decreased; OA also produced a decrease in phosphorylation of a 24 kDa protein. 3. Both substances produced an alteration in the proportions of bands of masses 170, 65 and 24 kDa, relative to total phosphorylation; PMA treatment also affected the band of mass 135 kDa. 4. Placental cell extracts were also subjected to Western blotting with a protein kinase C (PKC) antibody, reportedly specific for the alpha- and beta-isoforms. 5. Two immunoreactive proteins were detected; an 80 kDa band, presumably corresponding to the alpha- or beta-PKC, and a 64 kDa protein, which could be a degradation production of the 80 kDa protein or it could correspond to another form of the enzyme. The expression of PKC did not change on treatment with PMA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Proteínas da Gravidez
/
Proteína Quinase C
/
Acetato de Tetradecanoilforbol
/
Carcinógenos
/
Éteres Cíclicos
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Int J Biochem
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
África do Sul