Characterization of the extracellular matrix-containing giant perivascular spaces in the NOD mouse thymus.
Diabetes
; 42(1): 134-40, 1993 Jan.
Article
em En
| MEDLINE
| ID: mdl-8093603
ABSTRACT
It is well established that the NOD mouse develops T-cell-dependent autoimmune type I diabetes that is abolished by neonatal Tx and enhanced by Tx at weaning. In a previous study, we demonstrated that the NOD thymus displays various abnormalities in the microenvironmental compartment, including abnormal distribution of epithelial cell subsets, precocious decline in thymic hormone production and formation of giant PVS. These latter structures present an internal ECM-containing network filled with T-cells and to a lesser extent B-cells. Herein we have investigated further the giant PVS and particularly the origin of the T-cells that colonize these structures. The thymic origin of intra-PVS T-cells was ascertained by distinct protocols. First, sublethal X-ray irradiation or HC treatment leading to cortical thymocyte depletion showed that intra-PVS lymphocytes were resistant, similar to medullary thymocytes. Second, adoptive transfer experiments that used newborn or adult irradiated Thy-1 congenic recipients demonstrated that intra-PVS accumulation of T-cells did not result from the reentry of peripheral mature T-cells into the thymus. Third, kinetic studies that used BrdUrd pulse chase revealed that labeled intra-PVS cells appear late, simultaneously with medullary thymocytes, and remain only transiently within the PVS. Thus, the kinetics of T-cell reconstitution of PVS was compatible with the progressive differentiation of T-cell precursors originating from the thymic cortex. In this respect, the giant PVS of the NOD mouse thymus may represent a useful model to study the relationships between trafficking thymocytes and ECM proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Linfócitos T
/
Fibronectinas
/
Matriz Extracelular
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Diabetes
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Brasil