Effect of serum on differentiation of porcine adipose stromal-vascular cells in primary culture.
Comp Biochem Physiol Comp Physiol
; 105(3): 485-92, 1993 Jul.
Article
em En
| MEDLINE
| ID: mdl-8101780
ABSTRACT
1. Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologically, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sangue
/
Tecido Adiposo
/
Meios de Cultura Livres de Soro
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol Comp Physiol
Ano de publicação:
1993
Tipo de documento:
Article