Field testing an enzyme-linked synthetic oligonucleotide probe for identification of Anopheles gambiae s.s. and An. arabiensis.
Ann Trop Med Parasitol
; 87(6): 595-601, 1993 Dec.
Article
em En
| MEDLINE
| ID: mdl-8122921
ABSTRACT
Tests were carried out in Kenya to determine whether the enzyme-linked synthetic oligonucleotide probe (pAna 1) developed for identifying species of the Anopheles gambiae complex could be used under field conditions. The An. arabiensis male-specific pAna 1 probe was able to identify all male larval instars and adult males. However, the non-radioactive assay was not sufficiently sensitive to identify male sperm DNA in all the mated female An. arabiensis. Although the ratio of An. arabiensis to An. gambiae s.s. identified with pAna 1 in males during the dry season was in agreement with the ratio in half-gravid females identified cytogenetically, the ratios were different during the wet season. This study demonstrates that the enzyme-linked DNA probe assay is applicable under field conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sondas de Oligonucleotídeos
/
Anopheles
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Africa
Idioma:
En
Revista:
Ann Trop Med Parasitol
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Quênia