Simultaneous determination of felbamate and four metabolites in rat cerebrospinal fluid by high-performance liquid chromatography.
J Chromatogr
; 622(2): 223-8, 1993 Dec 22.
Article
em En
| MEDLINE
| ID: mdl-8150869
ABSTRACT
An isocratic liquid chromatographic method for direct sample injection has been developed for the quantitation of felbamate and four metabolites in rat cerebrospinal fluid. The method uses 0.050- or 0.025-ml aliquots of cerebrospinal fluid diluted with equal volumes of internal standard. Chromatography is performed on a 150 mm x 4.6 mm I.D. Spherisorb ODS2, 3-microns HPLC column eluted with a phosphate buffer-acetonitrile-methanol (82012060, v/v/v) mobile phase and ultraviolet absorbance detection at 210 nm. The linear quantitation ranges are felbamate and the 2-hydroxy metabolite 0.195-200 micrograms/ml, the propionic acid metabolite 0.195-50.0 micrograms/ml, the p-hydroxy metabolite 0.781 to 50.0 micrograms/ml, and the monocarbamate metabolite 0.098-50.0 micrograms/ml.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Propilenoglicóis
/
Anticonvulsivantes
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1993
Tipo de documento:
Article