The linkage change of a knotting reaction catalysed by Tn3 resolvase.
J Mol Biol
; 239(1): 25-36, 1994 May 27.
Article
em En
| MEDLINE
| ID: mdl-8196046
ABSTRACT
Tn3 resolvase catalyses recombination between a pair of identical res sites, directly repeated on a supercoiled plasmid molecule. The normal in vitro product is a two-noded catenane. A plasmid with a single base substitution at one res site is converted to non-recombinant knots by resolvase; the first major product is a four-noded knot. The linkage change, delta Lk, on formation of the four-noded knot is +4. The formation of the observed knots from the mutant substrate, and this value of delta Lk were predicted by and in agreement with a "simple rotation" model for catalysis of strand exchange by resolvase. A number of alternative mechanisms for knotting by resolvase are inconsistent with the observed topological changes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Proteínas de Ligação a DNA
/
Modelos Genéticos
/
Conformação de Ácido Nucleico
/
Nucleotidiltransferases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido