Enzymatic O-methylation of catechol estrogens in red blood cells: differences in animal species and strains.
Steroids
; 58(11): 540-6, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8273118
ABSTRACT
Enzymatic O-methylation of catechol estrogens in red blood cells has been investigated with respect to species difference. In the presence of S-adenosylmethionine, 2- or 4-hydroxyestradiol (2-OHE2 or 4-OHE2) was incubated with blood lysate obtained from rats (five strains), guinea pigs, mice, rabbits, dogs, monkeys, and humans, respectively. The yielded guaiacols and unchanged substrate were determined by gas chromatography/mass spectrometry in a selected ion monitoring mode employing the corresponding 2H4-labeled compounds as internal standards. The total amounts of guaiacols formed from 2-OHE2 and 4-OHE2 were different, being the highest (79.6% and 38.1%) in monkeys and the lowest (5.1% and 1.9%) in humans. The ratios of isomeric guaiacols formed from 4-OHE2 (4Me/3Me) were 7.6-71, while those from 2-OHE2 (2Me/3Me) were 1.4-3.2. Thus, marked differences in O-methylation of catechol estrogens were observed among animal species, but no significant strain difference was detected in rats.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estrogênios de Catecol
/
Eritrócitos
Limite:
Adult
/
Animals
/
Humans
Idioma:
En
Revista:
Steroids
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão