Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin.
Int J Parasitol
; 23(8): 977-83, 1993 Dec.
Article
em En
| MEDLINE
| ID: mdl-8300306
ABSTRACT
Adult Fasciola hepatica secrete a cysteine proteinase capable of cleaving host IgG close to the papain cleaving site. The proteinase was separated by size permeation chromatography. Gelatin-substrate polyacrylamide gel electrophoresis analysis revealed that the proteinase migrates as 6 proteolytic bands in the apparent molecular size range 60-90 kDa. Based on pH profiles of activity, inhibition studies using diethylpyrocarbonate and the diazomethylketone Z-phe-ala-CHN2, and characterising the substrate specificity of the enzymes using fluorogenic peptide substrates we have shown that the 60-90-kDa proteinases are cathepsin L-like proteinases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Imunoglobulina G
/
Cisteína Endopeptidases
/
Catepsinas
/
Fasciola hepatica
Limite:
Animals
Idioma:
En
Revista:
Int J Parasitol
Ano de publicação:
1993
Tipo de documento:
Article