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Determination of the molecular mass of the native beta-cell sulfonylurea receptor.
Skeer, J M; Dégano, P; Coles, B; Potier, M; Ashcroft, F M; Ashcroft, S J.
Afiliação
  • Skeer JM; Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Headington, Oxford, UK.
FEBS Lett ; 338(1): 98-102, 1994 Jan 24.
Article em En | MEDLINE | ID: mdl-8307165
ABSTRACT
In the present study we have determined the molecular mass of the beta-cell sulfonylurea receptor in its native form by two different experimental approaches; gel filtration chromatography and radiation inactivation analysis. We first confirmed that the denatured photolabelled MIN6 beta-cell receptor had a molecular size of 141 +/- 2 kDa (mean +/- S.E., n = 8). Under non-denaturing conditions, using gel filtration chromatography, apparent molecular masses of 166 +/- 1 kDa (mean +/- S.E., n = 3) and 182 +/- 5 kDa (mean +/- S.E., n = 4) were determined for the photoaffinity-labelled and unlabelled sulfonylurea receptor, respectively. We conclude that in the solubilized state the receptor exists as a monomer. Radiation inactivation analysis indicated that the receptor has a target size of 250 +/- 30 kDa (mean +/- S.E., n = 7). This value for the molecular mass is larger than that obtained from SDS-PAGE following photolabelling of the receptor (141 kDa) suggesting that the beta-cell sulfonylurea receptor is composed of more than one subunit in the native membrane.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Droga / Canais de Potássio / Ilhotas Pancreáticas / Transportadores de Cassetes de Ligação de ATP / Canais de Potássio Corretores do Fluxo de Internalização Idioma: En Revista: FEBS Lett Ano de publicação: 1994 Tipo de documento: Article País de afiliação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Droga / Canais de Potássio / Ilhotas Pancreáticas / Transportadores de Cassetes de Ligação de ATP / Canais de Potássio Corretores do Fluxo de Internalização Idioma: En Revista: FEBS Lett Ano de publicação: 1994 Tipo de documento: Article País de afiliação: Reino Unido