Determination of the molecular mass of the native beta-cell sulfonylurea receptor.
FEBS Lett
; 338(1): 98-102, 1994 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-8307165
ABSTRACT
In the present study we have determined the molecular mass of the beta-cell sulfonylurea receptor in its native form by two different experimental approaches; gel filtration chromatography and radiation inactivation analysis. We first confirmed that the denatured photolabelled MIN6 beta-cell receptor had a molecular size of 141 +/- 2 kDa (mean +/- S.E., n = 8). Under non-denaturing conditions, using gel filtration chromatography, apparent molecular masses of 166 +/- 1 kDa (mean +/- S.E., n = 3) and 182 +/- 5 kDa (mean +/- S.E., n = 4) were determined for the photoaffinity-labelled and unlabelled sulfonylurea receptor, respectively. We conclude that in the solubilized state the receptor exists as a monomer. Radiation inactivation analysis indicated that the receptor has a target size of 250 +/- 30 kDa (mean +/- S.E., n = 7). This value for the molecular mass is larger than that obtained from SDS-PAGE following photolabelling of the receptor (141 kDa) suggesting that the beta-cell sulfonylurea receptor is composed of more than one subunit in the native membrane.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Droga
/
Canais de Potássio
/
Ilhotas Pancreáticas
/
Transportadores de Cassetes de Ligação de ATP
/
Canais de Potássio Corretores do Fluxo de Internalização
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido