Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
Vet Immunol Immunopathol
; 39(1-3): 85-91, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8310661
ABSTRACT
A monoclonal antibody (mAb), MM1A, that identifies a molecule expressed on a large percentage of bovine lymphocytes (60-80%) was examined to determine its specificity. Two and three colour immunofluorescence analysis using flow cytometry revealed the molecule is highly expressed on all CD4+ and CD8+ lymphocytes and lymphocytes that express WC1 and the gamma/delta TCR. In contrast to mAbs reactive with BoCD5, MM1A did not react with B lymphocytes. Biochemical analysis revealed that the mAb immunoprecipitates a molecular complex comprising a set of peptides with M(r) approximately 12, 16, 22, 32, 36, and 44 kDa under reducing conditions and an additional 96 kDa peptide complex under non-reducing conditions. The data indicate that MM1A recognizes the bovine orthologue of CD3 (BoCD3).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Linfócitos
/
Antígenos CD
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
1993
Tipo de documento:
Article