Methods for the quantitation of bromosulfophthalein and its glutathione conjugate in biological fluids.
Anal Biochem
; 212(1): 28-34, 1993 Jul.
Article
em En
| MEDLINE
| ID: mdl-8368504
ABSTRACT
This paper describes a solvent-gradient HPLC method which was developed for the quantitation of bromosulfophthalein (BSP) in erythrocyte and albumin containing (blood) perfusate samples, and of BSP and its glutathione conjugate (BSP-GSH) in bile samples obtained from rat liver perfusion experiments. Phenolphthalein was used as an internal standard. The cysteinyl-, N-acetylcysteinyl-, and cysteinylglycinyl-BSP conjugates did not interfere with the HPLC assay and cysteine, N-acetylcysteine, [3H]GSH, and [3H]GSSG eluted within the first 6 min postinjection onto the HPLC system. A spectrophotometric method was also developed for the rapid quantitation of BSP in blood perfusate; tracer [14C]urea was utilized as the internal standard. Although the spectrophotometric method was less sensitive than the HPLC method, good correlation was found to exist between the methods.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfobromoftaleína
/
Glutationa
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá