A differential heat-conduction microcalorimeter for heat-capacity measurements of fluids.
J Biochem Biophys Methods
; 26(2-3): 149-71, 1993 May.
Article
em En
| MEDLINE
| ID: mdl-8509603
ABSTRACT
A heat-conduction calorimeter has been developed for measuring small changes in heat capacity of milligram samples of membrane lipid dispersed in water as a function of temperature. The operation of the instrument is based on the principle that the thermal response of the sample to a short (10 s), electrically generated heat burst is a function of the diffusivity of the sample. Modeling studies of the instrument's performance have revealed that the output response after the heat burst is a function of only the heat capacity, rho Cp. Calibration of the instrument experimentally confirmed this behavior. This feature obviated the need to measure the thermal conductivity in order to determine rho Cp from the diffusivity equation, eta = lambda/rho Cp. The calorimeter has the following characteristics reproducibility of loading +/- 400 microJ/C degrees.cm3; baseline stability +/- 10 microJ/C degrees.cm3 per 36 h; resolution (+/- 1 S.D.) +/- 50 microJ/C degrees.cm3; sample size 600 microliters.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Calorimetria
/
Temperatura Alta
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
J Biochem Biophys Methods
Ano de publicação:
1993
Tipo de documento:
Article