Growth inhibition of human glioma cells by superinduced human interferon-beta.
Neurol Med Chir (Tokyo)
; 35(10): 719-22, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-8532125
ABSTRACT
Superinduction of human interferon-beta (HuIFN-beta) from human glioma cells has greater cytotoxicity than purified HuIFN-beta derived from fibroblasts. However, superinduction requires several reagents like polyIpolyC, cycloheximide, and actinomycin D, which may contaminate the conditioned medium and obscure the effect of superinduced HuIFN-beta. The present study used minimum doses of polyIpolyC and cycloheximide without actinomycin D to superinduce HuIFN-beta. The superinduced HuIFN-beta was purified by passing the medium through molecular sieve column chromatography. Fractionation of the eluate provided semipurified superinduced HuIFN-beta which demonstrated a growth inhibitory effect against both the U251-MG autologous human glioma cell line and the SK-MG-1 homologous glioma cell line. This effect was neutralized by addition of anti-HuIFN-beta monoclonal antibody (YSB-1). In a separate experiment, combinations of superinduction reagents were found not to have growth inhibitory effects because all inhibition in superinduced medium was completely neutralized by YSB-1. Superinduced HuIFN-beta has a pure growth inhibitory effect on both autologous and homologous glioma cells, so may affect autocrine secretion of cytokines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Divisão Celular
/
Interferon beta
/
Glioma
Limite:
Humans
Idioma:
En
Revista:
Neurol Med Chir (Tokyo)
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Japão