A rapid gas chromatographic method quantitating clozapine in human plasma or serum for the purpose of therapeutic monitoring.
J Anal Toxicol
; 19(7): 537-41, 1995.
Article
em En
| MEDLINE
| ID: mdl-8577174
ABSTRACT
A gas chromatographic method using nitrogen-phosphorus detection was developed to quantitate clozapine in plasma or serum. Methyl clonazepam was used as an internal standard. Sample preparation included a single-step extraction with ethyl acetate, which was injected directly onto a wide-bore capillary column. Within-run and total precision, measured as percent coefficient of variation, were determined at low, therapeutic, and high clozapine plasma concentrations. The within-run precision for the low, therapeutic, and high clozapine plasma samples was 5.2, 2.7, and 2.4%, respectively. The total precision for the low, therapeutic, and high clozapine plasma samples was 10.0, 2.6, and 2.0%, respectively. Analytical accuracy was evaluated by comparing quantitative results with those obtained from a reference laboratory. Those samples containing therapeutic or high concentrations agreed within 3%; the sample containing a subtherapeutic concentration differed by 11.9%. The limit of quantitation was determined to be 35 ng/mL, and the upper limit of linearity was 3000 ng/mL. No significant interferences were detected after testing more than two dozen drugs and metabolites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antipsicóticos
/
Cromatografia Gasosa
/
Monitoramento de Medicamentos
/
Clozapina
Limite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos