Identification of the functional region on the superantigen Yersinia pseudotuberculosis-derived mitogen responsible for induction of lymphocyte proliferation by using synthetic peptides.
FEBS Lett
; 390(2): 196-8, 1996 Jul 22.
Article
em En
| MEDLINE
| ID: mdl-8706858
ABSTRACT
Yersinia pseudotuberculosis-derived mitogen (YPM) is the unique Gram-negative bacillary superantigen known. In order to identify the regions on the YPM molecule involved in its superantigenic activity, seven overlapping peptides of the entire YPM molecule were synthesized and tested to evaluate their effects on the YPM-induced proliferation of human peripheral blood lymphocytes. A peptide corresponding to the N-terminal amino acid sequence (1-23) was found to inhibit YPM-induced lymphocyte proliferation in a concentration-dependent manner. The N-terminal peptide was found to show no inhibition of the proliferation induced by the other superantigen (staphylococcal enterotoxin B) or the other T-cell mitogen pertussis toxin, indicating that the inhibition is specific to YPM-induced proliferation. Thus, we have identified the N-terminal region (1-23) of the YPM as one of the functional regions responsible for its superantigenic activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Yersinia pseudotuberculosis
/
Ativação Linfocitária
/
Superantígenos
/
Mitógenos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão