Transient expression of luciferase in Entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions.
Mol Biochem Parasitol
; 74(1): 1-10, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-8719240
ABSTRACT
We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(3) relative light units per second (RLU s-1) when assayed representing a stimulation of 18,000-fold over the control vector. Comparison of differing constructs allowed us to conclude that the 5' and 3' ferredoxin sequences are both necessary for optimal luciferase expression from our vectors. Transcriptional initiation occurs within the consensus sequence ATTCA in both construct and chromosomal ferredoxin promoters.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Protozoários
/
Entamoeba histolytica
/
Ferredoxinas
/
Luciferases
Tipo de estudo:
Etiology_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Biochem Parasitol
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Reino Unido