Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect.

ABSTRACT

To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.