Monoclonal antibody against bovine Lactoferricin and its epitopic site.
J Vet Med Sci
; 58(12): 1227-9, 1996 Dec.
Article
em En
| MEDLINE
| ID: mdl-8996707
ABSTRACT
Bovine lactoferricin (LFcin B) is a strong antimicrobial peptide derived from N-lobe of lactoferrin. To study the immunochemical and structural properties of LFcin B, monoclonal antibody (mAb) was prepared and the amino acid sequence concerning with the binding to mAb has been identified. Mice injected with LFcin B showed no production of antibody specific to this peptide, whereas those with LFcin B-KLH conjugate produced anti-LFcin B antibodies. None of the mAb reacted with bovine lactoferrin C-lobe, human lactoferrin or LFcin H. By the reactivity of the mAb against the peptides synthesized on cellulose membranes using SPOTs and against chemically modified derivatives of LFcin B, the antigenic determinant of LFcin B was identified to be the sequence of "QWR".
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactoferrina
/
Antibacterianos
/
Epitopos
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
J Vet Med Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão