Morphometric and autoradiographic studies on the growth of red and white axial muscle fibres in the shark Etmopterus spinax.

ABSTRACT

The axial muscle growth of the shark Etmopterus spinax has been studied by means of morphometry, slide histology and autoradiography from radioactive thymidine and amino acids. Comparisons have been made between the red and white muscles fibres. While growing from 10 to 23 cm in length, the white muscle transverse sectional (ts) area increases 5.4 times, while the red area increases 3.7 times. The mean ts area of individual white fibres increases by 2.7, and the ts area of red fibres increases by 1.6. The number of white fibres does not increase significantly, while the red fibres increase slightly in number. Red fibres incorporate amino acids faster than the white fibres, and have a higher turnover rate. The myonuclei of the red fibres (satellite cell nuclei) incorporate thymidine more often than do those of white fibres, but the frequency of the satellite cells is similar in the two fibre types. Mitoses are extremely rare in the myonuclei. The nucleocytoplasmic ratio is higher in the red than in the white fibres.