Stability of cytochrome P4501A-associated enzyme activity in cryogenically stored teleost liver samples.
Chemosphere
; 34(9-10): 2029-39, 1997 May.
Article
em En
| MEDLINE
| ID: mdl-9159902
ABSTRACT
Induction of cytochrome P4501A (CYP1A), more specifically the associated enzyme activity aryl hydrocarbon hydroxylase (AHH), has been shown to be one of the most sensitive measures of exposure of vertebrate animals to a range of organic chemical contaminants. However, in order to reliably use this biochemical method for analyzing archived samples, the stability of the enzyme activity in storage must be ascertained. Stability of AHH activity was determined for both tissue sections and subcellular fractions of liver collected from English sole (Pleuronectes vetulus), and held in cryogenic storage for up to 24 months. Our overall recommendations for sample collection, handling, storage, and assay are given.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Linguado
/
Hidrocarboneto de Aril Hidroxilases
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Criopreservação
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Bancos de Espécimes Biológicos
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Sistema Enzimático do Citocromo P-450
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Fígado
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Chemosphere
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos