Dynamic molecular combing: stretching the whole human genome for high-resolution studies.
Science
; 277(5331): 1518-23, 1997 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-9278517
ABSTRACT
DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA for precise measurements ranging from 7 to 150 kilobases, of gaps within a contig, and of microdeletions in the tuberous sclerosis 2 gene on patients' DNA. The simplicity, reproducibility, and precision of this approach makes it a powerful tool for a variety of genomic studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Humano
/
Técnicas Genéticas
/
Mapeamento Cromossômico
/
Genoma Fúngico
/
Proteínas Musculares
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França