Effects of epidermal growth factor on preimplantation mouse embryos.
J Assist Reprod Genet
; 14(7): 404-11, 1997 Aug.
Article
em En
| MEDLINE
| ID: mdl-9285326
ABSTRACT
PURPOSE:
Our purpose was to clarify the effects of epidermal growth factor (EGF) on mouse preimplantation embryos.METHODS:
We examined the effect of EGF on two-cell and four-cell stage mouse embryos cultured in vitro. In preimplantation embryos, we analyzed the binding of 125I-EGF by autoradiography and EGF receptor mRNA by the reverse transcription-polymerase chain reaction.RESULTS:
At more than 0.005 ng/ml, EGF relieved the two-cell block and regulated the differentiation of morula-stage embryos. These effects were negated by antiserum. EGF did not exhibit any marked effect on embryos between the four-cell and the morula stages. Specific binding for EGF and EGF receptor mRNA was detected during and after the morula stage.CONCLUSIONS:
The effects of EGF on preimplantation mouse embryos differed according to the stage of development, promoting cleavage before the four-cell stage and regulating differentiation after the morula stage. This regulatory action is thought to be transmitted to the cells via EGF receptors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Fator de Crescimento Epidérmico
Limite:
Animals
Idioma:
En
Revista:
J Assist Reprod Genet
Assunto da revista:
GENETICA
/
MEDICINA REPRODUTIVA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Japão