Changes in 1,25-(OH)2D3 synthesis and its receptor expression in spleen cell subpopulations of mice infected with LPBM5 retrovirus.
Endocrinology
; 138(12): 5505-10, 1997 Dec.
Article
em En
| MEDLINE
| ID: mdl-9389537
ABSTRACT
This study examines the influence of chronic retroviral infection of mice with a LPBM5 virus mixture on the paracrine system involving immune cells and 1,25-(OH)2D3 in the spleen. Plasma ionized calcium, 25-(OH)D and 1,25-(OH)2D of infected mice were unchanged. In contrast, the specific binding of 1,25-(OH)2D3 to spleen cytosol and the number of monocyte/macrophages expressing 1,25-(OH)2D3 receptors (VDR) were markedly increased. The retroviral infection also influenced the local production of 1,25-(OH)2D3 in the spleen. It did not alter this production in monocyte/macrophages but increased that in isolated T cells. Isolated B cells in control mice did not produce 1,25-(OH)2D3, but they increased the ability of isolated T cells to produce this metabolite during coculture incubations. Infection altered this cell interaction as 1,25-(OH)2D3 production in infected T cells decreased when these cells were cocultured with infected B cells. Thus, chronic retroviral infection alters both the local vitamin D metabolism and VDR expression by immune cells in mice. These findings suggest close local interactions between 1,25-(OH)2D3 and immune system activation during retroviral infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baço
/
Calcitriol
/
Síndrome de Imunodeficiência Adquirida Murina
/
Receptores de Superfície Celular
Limite:
Animals
Idioma:
En
Revista:
Endocrinology
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França