Whole blood assay for examining lymphocyte blastogenesis of percichthyid bass (Morone) [corrected].
Vet Immunol Immunopathol
; 58(3-4): 355-62, 1997 Sep 19.
Article
em En
| MEDLINE
| ID: mdl-9436278
ABSTRACT
A simple and reproducible method was developed for the measurement of blastogenesis of peripheral blood lymphocytes using whole blood of hybrid bass (striped bass [Morone saxatilis] female x white bass [M. chrysops] male) stimulated with Concanavalin A, phytohemagglutinin-P, lipopolysaccharide or pokeweed mitogen. Compared to traditional methods which use leucocyte separation procedures, whole blood culture is faster and less expensive. Only small aliquots of blood (10 microliters per culture well) were needed, which would be beneficial for sampling small fish as well as for taking multiple samples from single animals. Optimal culture conditions for hybrid bass, including mitogen concentration, incubation temperature and incubation period, were determined. This is the first report to demonstrate a blastogenic response of whole blood cells in fish.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bass
/
Ativação Linfocitária
Limite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos