Role of endogenous pro-enkephalin A-derived peptides in human T cell proliferation and monocyte IL-6 production.
J Neuroimmunol
; 84(1): 53-60, 1998 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-9600708
ABSTRACT
In this paper, we describe that met-enkephalin and/or enkephalin-containing intermediary peptides of the prohormone pro-enkephalin A are produced and secreted by human peripheral blood T cells and monocytes. The peptides are produced after stimulation with the mitogenic monoclonal antibodies anti-CD2.1/2.2 and anti-CD28. In monocytes, enkephalin synthesis was induced by stimulation with lipopolysaccharide. We demonstrate here that these immune cell-derived enkephalins play an important regulatory role in the immune response. By using an anti-sense oligonucleotide strategy we could block the production of enkephalins. Blockade of the production of met-enkephalin and enkephalin-containing intermediary peptides resulted in enhancement of the proliferative T cell response and inhibition of monocyte IL-6 secretion. In vitro reconstitution of the anti-sense treated cultures with synthetic met-enkephalin or the delta-type specific opioid receptor agonist deltorphin could reverse inhibition of monocyte IL-6 production, suggesting that endogenous enkephalins act via membrane opioid receptors. In contrast, addition of met-enkephalin or deltorphin to the anti-sense treated T cell cultures did not have any effect on T cell proliferation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Encefalinas
/
Monócitos
/
Linfócitos T
/
Interleucina-6
Limite:
Humans
Idioma:
En
Revista:
J Neuroimmunol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Holanda