Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice.
Vaccine
; 16(8): 768-74, 1998 May.
Article
em En
| MEDLINE
| ID: mdl-9627933
ABSTRACT
Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase that catalyses the transfer of sialic acid from host glycoconjugates to the parasite surface. Several lines of evidence suggest that this enzyme is a virulence factor implicated in the establishment of infection. Here we studied whether immunization with a plasmid DNA containing a gene encoding for the catalytic domain of the enzyme could elicit protective immunity against T. cruzi infection in mice. We observed that immunization with this plasmid DNA generated antibody and T-cell mediated immune responses. Antibodies recognized the native enzyme and inhibited its activity in vitro. Upon challenge with bloodstream trypomastigotes, immunized animals displayed reduced parasitemia and mortality.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Glicoproteínas
/
Vacinas Protozoárias
/
Doença de Chagas
/
Vacinas de DNA
/
Antígenos de Protozoários
/
Neuraminidase
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Brasil