DNA compression caused by an upstream point mutation.
Biotechniques
; 25(1): 68-72, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9668978
ABSTRACT
We observed an apparent series of insertions and deletions beginning 5 bp downstream of an A-->G silent transition in exon 1 of the tumor necrosis factor receptor 1 gene. The apparent sequence anomaly was observed only in individuals carrying the transition. Formamide gel electrophoresis revealed that the apparent sequence anomaly was due to compression. The compression is plausibly explained by a hairpin in the reaction products in a region of trinucleotide CAG repeats. One should suspect the presence of DNA compression when a series of deletions and insertions follows a single base pair mutation that leads to a series of trinucleotide repeats.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Antígenos CD
/
Mutação Puntual
/
Receptores do Fator de Necrose Tumoral
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos