A nitrocellulose membrane-based ELISA for the detection of Plasmodium infections in mosquitos
Bulletin of the World Health Organization (WHO)
; 67(5): 525-33, 1989. graf
Article
em En
| PAHO
| ID: pah-7348
Biblioteca responsável:
US1.1
Localização: US1.1, WHO COLL
ABSTRACT
A nitrocellulose (NC) membrane was evaluated as a solid-phase support for the detection of malaria-infected mosquitos using monoclonal antibodies (MAb) with a laboratory model based on Plasmodium inui and Anopheles dirus. MAbs produced against sporozoites of the N34 strain of P. inui, and selected by immunofluorescence assay and the circumsporozoite precipitin test, were used. A one-site indirect NC-ELISA that used unlabelled MAb and enzyme-labelled anti-mouse IgG was developed. Its sensitivity was about 200 sporozoites and it reliably detected one infected mosquito in a pool of 20. This indirect NC-ELISA has the advantage that it does not require direct conjugation of the MAb to an enzyme or biotin. In the direct one-site NC-ELISA, which is also reported, the relatively simple biotinylation procedure was an alternative to the enzyme- or radiolabelled MAbs
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Coleções:
04-international_org
Base de dados:
PAHO
Assunto principal:
Plasmodium falciparum
/
Ensaio de Imunoadsorção Enzimática
/
Malária
/
Anticorpos Monoclonais
Idioma:
En
Revista:
Bulletin of the World Health Organization (WHO)
Ano de publicação:
1989
Tipo de documento:
Article