HIV Treatment Outcomes in POP-UP: Drop-in HIV Primary Care Model for People Experiencing Homelessness.

BACKGROUND: People with HIV experiencing homelessness have low rates of viral suppression, driven by sociostructural barriers and traditional care system limitations. Informed by the capability-opportunity-motivation-behavior (COM-B) model and patient preference research, we developed POP-UP, an integrated drop-in (nonappointment-based) HIV clinic with wrap-around services for persons with housing instability and viral nonsuppression in San Francisco. METHODS: We report HIV viral suppression (VS; <200 copies/mL), care engagement, and mortality at 12 months postenrollment. We used logistic regression to determine participant characteristics associated with VS. RESULTS: We enrolled 112 patients with viral nonsuppression and housing instability: 52% experiencing street-homelessness, 100% with a substance use disorder, and 70% with mental health diagnoses. At 12 months postenrollment, 70% had ≥1 visit each 4-month period, although 59% had a 90-day care gap; 44% had VS, 24% had viral nonsuppression, 23% missing, and 9% died (6 overdose, 2 AIDS-associated, 2 other). No baseline characteristics were associated with VS. CONCLUSIONS: The POP-UP low-barrier HIV care model successfully reached and retained some of our clinic's highest-risk patients. It was associated with VS improvement from 0% at baseline to 44% at 12 months among people with housing instability. Care gaps and high mortality from overdose remain major challenges to achieving optimal HIV treatment outcomes in this population.

Xylem embolism spread is largely prevented by interconduit pit membranes until the majority of conduits are gas-filled.

Xylem embolism resistance varies across species influencing drought tolerance, yet little is known about the determinants of the embolism resistance of an individual conduit. Here we conducted an experiment using the optical vulnerability method to test whether individual conduits have a specific water potential threshold for embolism formation and whether pre-existing embolism in neighbouring conduits alters this threshold. Observations were made on a diverse sample of angiosperm and conifer species through a cycle of dehydration, rehydration and subsequent dehydration to death. Upon rehydration after the formation of embolism, no refilling was observed. When little pre-existing embolism was present, xylem conduits had a conserved, individual embolism-resistance threshold that varied across the population of conduits. The consequence of a variable conduit-specific embolism threshold is that a small degree of pre-existing embolism in the xylem results in apparently more resistant xylem in subsequent dehydrations, particularly in angiosperms with vessels. While our results suggest that pit membranes separating xylem conduits are critical for maintaining a conserved individual conduit threshold for embolism when little pre-existing embolism is present, as the percentage of embolized conduits increases, gas movement, local pressure differences and connectivity between conduits increasingly contribute to embolism spread.

Limited plasticity in embolism resistance in response to light in leaves and stems in species with considerable vulnerability segmentation.

Xylem resistance to embolism is a key metric determining plant survival during drought. Yet, we have a limited understanding of the degree of plasticity in vulnerability to embolism. Here, we tested whether light availability influences embolism resistance in leaves and stems. The optical vulnerability method was used to assess stem and leaf resistance to embolism in Phellodendron amurense and Ilex verticillata acclimated to sun and shade microenvironments within the same canopy. In both species, we found considerable segmentation in xylem resistance to embolism between leaves and stems, but only minor acclimation in response to light availability. With the addition of a third species, Betula pubescens, which shows no vulnerability segmentation, we sought to investigate xylem anatomical traits that might correlate with strong vulnerability segmentation. We found a correlation between the area fraction of vessels in the xylem and embolism resistance across species and tissue types. Our results suggest that minimal acclimation of embolism resistance occurs in response to light environment in the same individual and that the degree of vulnerability segmentation between leaves and stems might be determined by the vessel lumen fraction of the xylem.

Selenium downregulates auxin and ethylene biosynthesis in rice seedlings to modify primary metabolism and root architecture.

MAIN CONCLUSION: Selenium modulates the formation of primary and lateral roots through alterations in auxin and ethylene, leading to new patterns of root architecture in rice seedlings. Selenium (Se) at low concentrations can control root growth through interaction with hormone biosynthesis. Auxin and ethylene have been shown to control the root architecture, with most of the information obtained from the eudicots such Arabidopsis and Nicotiana tabacum. Here, we presented the effects of Se on auxin and ethylene pathways and examined their impact on primary metabolism and root system architecture in rice (Oryza sativa L.) seedlings. Se treatment increased elongation of primary root, but decreased the number and length of lateral roots. Se led to decreased expression of genes associated with the biosynthesis of auxin and ethylene, concomitantly with reduced production of these hormones by the roots. Moreover, Se decreased the abundance of transcripts encoding auxin transport proteins. Indole-3-acetic acid (IAA) treatment overrode the repressive effect of Se on lateral root growth. The ethylene synthesis inhibitor L-α-(2-aminoethoxyvinyl)-glycine (AVG) increased elongation of primary root, whereas the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) resulted in the opposite effect. Soluble sugars accumulate in roots of rice seedlings under Se treatment. Thus, Se modulates the formation of primary and lateral roots through alterations in auxin and ethylene, leading to new patterns of root architecture in rice seedlings.

Photosynthetic and metabolic acclimation to repeated drought events play key roles in drought tolerance in coffee.

Over the last decades, most information on the mechanisms underlying tolerance to drought has been gained by considering this stress as a single event that happens just once in the life of a plant, in contrast to what occurs under natural conditions where recurrent drought episodes are the rule. Here we explored mechanisms of drought tolerance in coffee (Coffea canephora) plants from a broader perspective, integrating key aspects of plant physiology and biochemistry. We show that plants exposed to multiple drought events displayed higher photosynthetic rates, which were largely accounted for by biochemical rather than diffusive or hydraulic factors, than those submitted to drought for the first time. Indeed, these plants displayed higher activities of RuBisCO and other enzymes associated with carbon and antioxidant metabolism. Acclimation to multiple drought events involved the expression of trainable genes related to drought tolerance and was also associated with a deep metabolite reprogramming with concordant alterations in central metabolic processes such as respiration and photorespiration. Our results demonstrate that plants exposed to multiple drought cycles can develop a differential acclimation that potentiates their defence mechanisms, allowing them to be kept in an 'alert state' to successfully cope with further drought events.

The relative area of vessels in xylem correlates with stem embolism resistance within and between genera.

The resistance of xylem conduits to embolism is a major factor defining drought tolerance and can set the distributional limits of species across rainfall gradients. Recent work suggests that the proximity of vessels to neighbors increases the vulnerability of a conduit. We therefore investigated whether the relative vessel area of xylem correlates with intra- and inter-generic variation in xylem embolism resistance in species pairs or triplets from the genera Acer, Cinnamomum, Ilex, Quercus and Persea, adapted to environments differing in aridity. We used the optical vulnerability method to assess embolism resistance in stems and conducted anatomical measurements on the xylem in which embolism resistance was quantified. Vessel lumen fraction (VLF) correlated with xylem embolism resistance across and within genera. A low VLF likely increases the resistance to gas movement between conduits, by diffusion or advection, whereas a high VLF enhances gas transport thorough increased conduit-to-conduit connectivity and reduced distances between conduits and therefore the likelihood of embolism propagation. We suggest that the rate of gas movement due to local pressure differences and xylem network connectivity is a central driver of embolism propagation in angiosperm vessels.

The interplay between irrigation and fruiting on branch growth and mortality, gas exchange and water relations of coffee trees.

The overall coordination between gas exchanges and plant hydraulics may be affected by soil water availability and source-to-sink relationships. Here we evaluated how branch growth and mortality, leaf gas exchange and metabolism are affected in coffee (Coffea arabica L.) trees by drought and fruiting. Field-grown plants were irrigated or not, and maintained with full or no fruit load. Under mild water deficit, irrigation per se did not significantly impact growth but markedly reduced branch mortality in fruiting trees, despite similar leaf assimilate pools and water status. Fruiting increased net photosynthetic rate in parallel with an enhanced stomatal conductance, particularly in irrigated plants. Mesophyll conductance and maximum RuBisCO carboxylation rate remained unchanged across treatments. The increased stomatal conductance in fruiting trees over nonfruiting ones was unrelated to internal CO2 concentration, foliar abscisic acid (ABA) levels or differential ABA sensitivity. However, stomatal conductance was associated with higher stomatal density, lower stomatal sensitivity to vapor pressure deficit, and higher leaf hydraulic conductance and capacitance. Increased leaf transpiration rate in fruiting trees was supported by coordinated alterations in plant hydraulics, which explained the maintenance of plant water status. Finally, by preventing branch mortality, irrigation can mitigate biennial production fluctuations and improve the sustainability of coffee plantations.

Elevated [CO2] benefits coffee growth and photosynthetic performance regardless of light availability.

Despite being evolved in shaded environments, most coffee (Coffea arabica L.) is cultivated worldwide under sparse shade or at full sunlight. Coffee is ranked as greatly responsive to climate change (CC), and shading has been considered an important management strategy for mitigating the harmful CC outcomes on the crop. However, there is no information on the effects of enhanced [CO2] (eCa) on coffee performance in response to light availability. Here, we examined how carbon assimilation and use are affected by eCa in combination with contrasting light levels. For that, greenhouse-grown plants were submitted to varying light levels (16 or 7.5 mol photons m-2 day-1) and [CO2] (ca. 380 or 740 µmol mol-1 air) over six months. We demonstrated that both high light and eCa improved growth and photosynthetic performance, independently. Despite marginal alterations in biomass partitioning, some allometric changes, such as higher root biomass-to-total leaf area and lower leaf area ratio under the combination of eCa and high light were found. Stimulation of photosynthetic rates by eCa occurred with no direct effect on stomatal and mesophyll conductances, and no signs of photosynthetic down-regulation were found irrespective of treatments. Particularly at high light, eCa led to decreases in both photorespiration rates and oxidative pressure. Overall, our novel findings suggest that eCa could tandemly act with shading to mitigate the harmful CC effects on coffee sustainability.

Salinity-induced modifications on growth, physiology and 20-hydroxyecdysone levels in Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen].

- Salinity is a major threat to agriculture. However, depending on the concentration of soluble salts in soil, increased secondary metabolite levels can occur with no major damages to plant growth and development. The phytoecdysteroid (PE) 20-hydroxyecdysone (20E) is a secondary metabolite with biotechnological, medicinal, pharmaceutical and agrochemical applicability. Here, we characterize the responses (growth and physiology) of Pfaffia glomerata under different NaCl concentrations and examine the production of 20E as affected by salinity. Forty-day-old plants grown in greenhouse were exposed to 0, 120, 240, 360 or 480 mM of NaCl for 11 days. Moderate salinity (i.e., 120 mM of NaCl) led to increased 20E concentrations in leaves (47%) relative to the control with no significant effect on photosynthesis and biomass accumulation, thus allowing improved 20E contents on a per whole-plant basis. In contrast, plants under high salinity (i.e., 240-480 mM of NaCl) displayed similar 20E concentrations in leaves compared to the control, but with marked impairments to biomass accumulation and photosynthetic performance (coupled with decreased sucrose and starch levels) in parallel to nutritional imbalance. High salinity also strongly increased salicylic acid levels, antioxidant enzyme activities, and osmoregulatory status. Regardless of stress severity, 20E production was accompanied by the upregulation of Spook and Phantom genes. Our findings suggest that P. glomerata cultivation in moderate salinity soils can be considered as a suitable agricultural option to increase 20E levels, since metabolic and structural complexity that makes its artificial synthesis very difficult.

Physiological and Agronomic Performance of the Coffee Crop in the Context of Climate Change and Global Warming: A Review.

Coffee is one of the most important global crops and provides a livelihood to millions of people living in developing countries. Coffee species have been described as being highly sensitive to climate change, as largely deduced from modeling studies based on predictions of rising temperatures and changing rainfall patterns. Here, we discuss the physiological responses of the coffee tree in the context of present and ongoing climate changes, including drought, heat, and light stresses, and interactions between these factors. We also summarize recent insights on the physiological and agronomic performance of coffee at elevated atmospheric CO2 concentrations and highlight the key role of CO2 in mitigating the harmful effects of heat stress. Evidence is shown suggesting that warming, per se, may be less harmful to coffee suitability than previously estimated, at least under the conditions of an adequate water supply. Finally, we discuss several mitigation strategies to improve crop performance in a changing world.

Silicon improves rice grain yield and photosynthesis specifically when supplied during the reproductive growth stage.

Silicon (Si) has been recognized as a beneficial element to improve rice (Oryza sativa L.) grain yield. Despite some evidence suggesting that this positive effect is observed when Si is supplied along the reproductive growth stage (from panicle initiation to heading), it remains unclear whether its supplementation during distinct growth phases can differentially impact physiological aspects of rice and its yield and the underlying mechanisms. Here, we investigated the effects of additions/removals of Si at different growth stages and their impacts on rice yield components, photosynthetic performance, and expression of genes (Lsi1, Lsi2 and Lsi6) involved in Si distribution within rice shoots. Positive effects of Si on rice production and photosynthesis were manifested when it was specifically supplied during the reproductive growth stage, as demonstrated by: (1) a high crop yield associated with higher grain number and higher 1000-grain weight, whereas the leaf area and whole-plant biomass remained unchanged; (2) an increased sink strength which, in turn, exerted a feed-forward effect on photosynthesis that was coupled with increases in both stomatal conductance and biochemical capacity to fix CO2; (3) higher Si amounts in the developing panicles (and grain husks) in good agreement with a remarkable up-regulation of Lsi6 (and to a lesser extent Lsi1). We suggest that proper levels of Si in these reproductive structures seem to play an as yet unidentified role culminating with higher grain number and size.

Psychosis-related matricide associated with a lesion of the ventromedial prefrontal cortex.

Matricide, the killing of a mother by her biological child, is a rare event. We report a case of matricide associated with a woman who sustained a right ventromedial prefrontal lesion during surgery for nasal polyposis that was performed when she was 40 years old. After her surgery, she developed psychotic symptoms associated with the emergence of antisocial behavior. She committed matricide 22 years later. Neuropsychological evaluation showed decreased frontal-executive deficits, and magnetic resonance imaging revealed a lesion in the right gyrus rectus area of the ventromedial prefrontal region. This case suggests that a secondary psychotic syndrome associated with a lesion in the frontal neural network, which is disturbed in psychopathy, could facilitate homicidal behavior. Furthermore, this case has legal implications for the prosecution of murder associated with a brain lesion.

Identidade pela fotografia: construção e resgate da identidade na Comunidade Novo Aarão Reis / Identity through photography: identity construction and rescue of the Community Novo Aarão Reis / Identidad a través de la fotografía: construcción de la identidad y el rescate de la Comunidad Novo Aarão Reis

Este artigo relata uma experiência inovadora de montagem expositiva itinerante de fotografias, que foi realizada no Centro de Saúde da Comunidade do Novo Aarão Reis, Município de Belo Horizonte, em Minas Gerais. Tem como objetivo apresentar uma forma de construção, resgate e manutenção da memória, história e identidade dos moradores da comunidade, na medida em que transforma a percepção dos moradores sobre si mesmos e sobre o mundo que os cerca. Ainda, expõe estes moradores a outros de outras localidades da imensa "aldeia global", fazendo surgir, sobre a coletividade, a percepção e a legitimidade de pertencer ao Novo Aarão Reis, não só de maneira física, mas também imaginativamente na perspectiva do simplesmente ser e pertencer.

This article reports an innovative experience of mounting a traveling exhibition of photographs, which was held at the Community Health Center of Novo Aarão Reis, Municipality of Belo Horizonte, in Minas Gerais. It aims to present a form of construction, rescue, and maintenance of memory, history, and identity of the community residents, as it transforms the perception of residents about themselves and the world around them. Furthermore, it exposes these residents to others from elsewhere in our vast "global village", giving rise to the perception of the community and the legitimacy of belonging to the Novo Aarão Reis, not only in a physical way, but also imaginatively, "simply being and belonging".

Este artículo relata una experiencia innovadora para crear una exposición itinerante de fotografías que se realizó en el Centro de Salud de La Comunidad de Novo Aarão Reis, Municipio de Belo Horizonte, en Minas Gerais. Su objetivo es presentar una forma de construcción, rescate y mantenimiento de la memoria, de la historia y de la identidad de los residentes de la comunidad en la que transforma la percepción de los residentes acerca de sí mismos y al mundo que les rodea. Además, expone ellos a otras comunidades de nuestra "aldea global", dando lugar a la percepción y la legitimidad de pertenecer a la comunidad de Novo Aarão Reis, no sólo de una manera física, sino también con imaginación de simplemente "ser con pertenencia"

Estudio de las características de la ingesta alcohólica de un grupo de adolescentes de la comuna de Arauco / Study about the characteristics of alcoholic drinking in an adolescent group of the Arauco community

Se estudiaron 305 adolescentes de ambos sexos, pertenecientes a los cursos de 1o. a 4o. año de Educación media del Liceo A-50 de Arauco, observándose un inicio tardío de la ingesta etílica, una baja influencia del medio familiar en la conducta alcohólica de adolescentes y una gran importancia de factores externos como son fiestas, discotheques y amigos