Synthesis, characterization and application of intracellular Ag/AgCl nanohybrids biosynthesized in Scenedesmus sp. as neutral lipid inducer and antibacterial agent.

The current research focuses on the Intracellular biosynthesis of Ag/AgCl nanohybrids in microalgae, Scenedesmus sp. The effect of biosynthesis process on growth and lipid profile of cells is key element of this study. Ag/AgCl nanohybrids synthesized intracellularly were characterized by UV-Vis spectrophotometer, Powder X-Ray Diffraction (P-XRD), Scanning Electron Microscopy (SEM), High Resolution Transmission Electron Microscopy (HRTEM). 10-20 nm and 10-50 nm sized spherical shaped nanoparticles of polycrystalline nature were grown using 0.5 and 1 mM of AgNO3 precursor, respectively and Scenedesmus sp. as reducing agent. Total lipid content of the cells treated with 0.5 mM and 1 mM AgNO3 was static and found to be 43.2 ± 0.01 µg/mL and 48.2 ± 0.02 µg/mL respectively at 120 h of Ag/AgCl nanoparticles biosynthesis. FAME (Fatty Acid Methyl Ester) profile was improved due to intracellular nanoparticles biosynthesis with maximum C16:0 (palmitic acid) (35.7%) in cells treated with 0.5 mM AgNO3 used for Ag/AgCl nanohybrids synthesis. Palmitic acid in cells exposed to 0.5 mM concentration of metallic precursor increased by 75.86%. Synthesized nanoparticles were tested on four bacterial strains to establish its antibacterial efficiency showing appropriate zone of inhibition at varying concentrations. Present study efficiently demonstrates the utility of microalgae integrating nanoparticles biosynthesis and lipid accumulation.

Anaerobic digestion as a tool to manage eutrophication and associated greenhouse gas emission.

Eutrophicated inland water bodies are noticed to be one of the contributing factors to greenhouse gas (GHGs) emissions. Direct discharge of untreated or partially treated water is a major concern. Microalgae-based technology and management are regarded as one of the potential nature-based approaches to combat eutrophication. In turn, the microalgae facilitate the recovery of GHGs contributing compounds in the form of organic biomass. The recovered algal biomass can be harnessed for the production of biofuels and other bio-products, like biofertilizer, using anaerobic digestion. By virtue, circular bio-economy can be achieved alongside mitigating GHGs emissions. Before implementing, it is vital to thoroughly explore the links between the process and potential alternatives for wastewater treatment, waste valorization, biofuel production, and land usage. Thus, the present review discusses the impact of eutrophication on ecology and environment, current technologies for mitigating eutrophication and GHGs, and energy recovery through the anaerobic digestion of algal biomass. Further, the processes at the intercept of wastewater treatment and biogas production were reviewed to leverage the potential of anaerobic digestion for making a circular bioeconomy framework.

The use of response surface methodology for improving fatty acid methyl ester profile of Scenedesmus vacuolatus.

The present study has been designed to optimise certain important process parameters for Scenedesmus vacuolatus to achieve efficient carbon dioxide extenuation as well as suitable fatty acid profile in context to improve biodiesel properties. The effect of varying sodium bicarbonate concentration was evaluated in single and multicomponent system such as nitrate, phosphate, inoculum size to observe interactive effects on algae biomass production, carbon dioxide (CO2) removal efficiency and fatty acid methyl ester (FAME) profile. Maximum biomass productivity of 117.0 ± 7.7 mg/L/day with 3 g/L of sodium bicarbonate was obtained i.e. approximately 2 folds higher than the control. Under multicomponent exposure, maximum biomass of 1701.5 ± 88.8 mg/L and maximum chlorophyll concentration of 15.3 ± 6.4 mg/L were achieved on 14th day at 3 g/L sodium nitrate, 0.1 g/L dipotassium hydrogen phosphate, 2 g/L of sodium bicarbonate and initial cell density of 0.3 (N3P0.1B2OD0.3). FAME content of 46.1 mg/g of biomass was obtained at this combination which is approximately 3 folds higher than the FAME content obtained under nitrogen and phosphate deprivation (16.6 mg/g at N0P0B2OD0.3). Confocal microscopy images confirmed the results with enhanced lipid droplet accumulation at high bicarbonate concentration as compared with the control. This interactive study concluded the variability in FAME profile along with the exposure to varying nutrient concentrations.

Screening of microalgae for biosynthesis and optimization of Ag/AgCl nano hybrids having antibacterial effect.

Here we report a facile and novel bio-synthesis technique, using algal extract to reduce silver metal ions into Ag/AgCl nanoparticles. Different concentrations of metallic precursors of silver nitrate (0.1 mM, 0.2 mM, 0.5 mM and 1 mM) were tested with alcoholic extract prepared from biomass of Chlorella sp. for nanoparticle biosynthesis which was screened out of four species namely Chlorella sp., Lyngbya putealis, Oocystis sp. and Scenedesmus vacuolatus. The biomolecules present in the alcoholic extract assisted in the synthesis of nanoparticles by reducing the metallic salt to metal ions and acting as capping agents in order to stabilize the particles. The synthesized particles were characterized for physico-chemical properties. DLS analysis of particles prepared from Chlorella sp. shows the particles with size of 90.6 nm. These biosynthesized nanoparticles show great potential applications in antibacterial activity.

Perinuclear antineutrophil cytoplasmic autoantibodies and anti-Saccharomyces cerevisiae antibodies as serological markers are not specific in the identification of Crohn's disease and ulcerative colitis.

OBJECTIVE: To evaluate the diagnostic accuracy of perinuclear antineutrophil cytoplasmic autoantibodies (pANCAs) and anti-Saccharomyces cerevisiae antibodies (ASCAs), as single agents and in combination, for the diagnosis of Crohn's disease (CD) and ulcerative colitis (UC), including in cases of indeterminate colitis (IC). METHODS: The sera from a total of 98 patients were studied: 77 with CD, 16 with UC and five with IC. The medical records of these patients were reviewed for disease diagnosis, demographic data, and patient symptoms and medications. ELISAs were utilized to detect the presence of ASCAs and deoxyribonuclease-sensitive pANCAs, and these results were then compared with the patients' clinical data. RESULTS: For UC, a positive pANCA test alone provided a sensitivity of 50% and a specificity of 82%. For CD, a positive ASCA test alone provided a sensitivity of 40% and a specificity of 100%. A combination of pANCA-positive and ASCA-negative results showed a sensitivity of 50% and specificity of 90% for the diagnosis of UC. Similarly, the combination of ASCA-positive and pANCA-negative results provided a sensitivity and specificity of 32% and 100% for the diagnosis of CD, respectively. Interestingly, 80% of IC patients showed serology results consistent with UC. CONCLUSIONS: Although this combination of serological markers provides a diagnostic tool with generally high specificities, the low sensitivities of these serological markers, most notably in terms of CD, preclude the possibility that they can replace the tools currently used for inflammatory bowel disease diagnosis and management. It is possible, however, that these serological markers may prove beneficial in the management of IC.

Proteomic approaches in microalgae: perspectives and applications.

Biofuels are the promising sources which are produced by various microalgae or in the form of metabolic by-products from organic or food waste products. Microalgae have been widely reported for the production of biofuels since these have a high storage of lipids as triacylglycerides, which can mainly be converted into biofuels. Recently, products such as biodiesel, bioethanol and biogas have renewed the interest toward the microalgae. The proteomics alone will not pave the way toward finding an ideal alga which will fulfill the current energy demands, but a combined approach of proteomics, genomics and bioinformatics can be pivotal for a sustainable solution. The present review emphasizes various technologies currently involved in algal proteomics for the efficient production of biofuels.

Esthetic dentistry for multiple gingival recession cases: Coronally advanced flap with bracket application.

Treatment of gingival recession is essential to rectify the esthetic and functional deficiencies of the patient and to combat further periodontal destruction. However, treating multiple recession cases is quite challenging, and therefore requires constant modifications of the prevalent treatment strategies as per the severity of the condition. The objective of this case report was to evaluate the effectiveness of coronally advanced flap (CAF) technique without vertical incisions using CAF brackets (CAF+B) for treating a patient presenting with class II gingival recession defects in relation to maxillary anteriors. Complete root coverage was observed, and the results were consistent even after 6 months. The current case report demonstrates good outcomes of the CAF + B technique without the use of any additional soft tissue grafts or vertical incisions, therefore, endorsing the promising potential of the CAF + B technique in multiple gingival recession cases.

Periodontium bestows vision!!

The role of periodontium in supporting the tooth structures is well-known. However, less is known about its contribution to the field of ophthalmology. Corneal diseases are among major causes of blindness affecting millions of people worldwide, for which synthetic keratoprosthesis was considered the last resort to restore vision. Yet, these synthetic keratoprosthesis suffered from serious limitations, especially the foreign body reactions invoked by them resulting in extrusion of the whole prosthesis from the eye. To overcome these shortcomings, an autologous osteo-odonto keratoprosthesis utilizing intraoral entities was introduced that could positively restore vision even in cases of severely damaged eyes. The successful functioning of this prosthesis, however, predominantly depended on the presence of a healthy periodontium for grafting. Therefore, the following short communication aims to acknowledge this lesser-known role of the periodontium and other oral structures in bestowing vision to the blind patients.

Computerized implant-dentistry: Advances toward automation.

Advancements in the field of implantology such as three-dimensional imaging, implant-planning software, computer-aided-design/computer-aided-manufacturing (CAD/CAM) technology, computer-guided, and navigated implant surgery have led to the computerization of implant-dentistry. This three-dimensional computer-generated implant-planning and surgery has not only enabled accurate preoperative evaluation of the anatomic limitations but has also facilitated preoperative planning of implant positions along with virtual implant placement and subsequently transferring the virtual treatment plans onto the surgical phase via static (guided) or dynamic (navigated) systems aided by CAD/CAM technology. Computerized-implant-dentistry being highly predictable and minimally invasive in nature has also allowed implant placement in patients with medical comorbidities (e.g. radiation therapy, blood dyscrasias), in patients with complex problems following a significant alteration of the bony anatomy as a result of benign or malignant pathology of the jaws or trauma and in patients with other physical and emotional problems. With significant achievements accomplished in the field of computerized implant-dentistry, attempts are now been made toward complete automation of implant-dentistry.

In Vivo Evaluation of Two-Piece Implants Placed Following One-Stage and Two-Stage Surgical Protocol in Posterior Mandibular Region. Assessment of Alterations in Crestal Bone Level.

BACKGROUND: Endosseous implants can be placed following either two-stage technique requiring second-stage surgery or one-stage technique, which does not involve a second surgical intervention. PURPOSE: The present study was undertaken to evaluate and compare the changes in crestal bone level when two-piece implants were placed in posterior mandibular region following one-stage and two-stage surgical protocol. MATERIALS AND METHODS: A parallel group randomized prospective study was designed in which 20 two-piece implants were placed in the posterior mandibular region of 16 partially edentulous healthy patients following either one-stage (Group I) or a two-stage surgical protocol (Group II). Alterations in crestal bone level were assessed with the help of DentaScan at baseline, that is, at the time of implant placement, third month and sixth month. RESULTS: Nonsignificant differences were seen in both groups in terms of changes in crestal bone level at the final evaluation. CONCLUSIONS: Hence, it could be concluded that two-piece implants can be placed following one-stage surgical protocol as predictably as when two-stage surgical technique is followed.

Factors predictive of changes in the legal status of psychiatric inpatients.

The objective of this study was to identify factors predictive of duration of involuntary hospitalization, i.e., factors that would predict early versus late conversion to voluntary status. Charts of 209 patients admitted involuntarily to an acute psychiatric inpatient unit were studied using a naturalistic, prospective design. Multivariate regression analysis was used to determine the effect of a number of variables in predicting duration of involuntary hospitalization. Of all variables studied, only smoking preference was found to be a statistically significant predictor of duration of involuntary hospitalization. Smokers were converted to voluntary status earlier than non-smokers. The authors concluded that clinicians may not be using commitment statutes as per recommended legal guidelines. Patients who smoke may be applying overt and covert pressure on clinicians with regard to conversion to voluntary status to gain smoking privileges.

Host modulation therapy: An indispensable part of perioceutics.

Traditionally, only antimicrobials have been used as the chemotherapeutic modality for the treatment of periodontitis. Though bacteria are the primary etiologic factors of periodontal diseases, yet the extent and severity of tissue destruction seen in periodontitis is determined by the host immuno-inflammatory response to these bacteria. This increasing awareness and knowledge of the host-microbial interaction in periodontal pathogenesis has presented the opportunity for exploring new therapeutic strategies for periodontitis by means of targeting host response via host-modulating agents. This has lead to the emergence of the field of "Perioceutics" i.e. the use of parmacotherapeutic agents including antimicrobial therapy as well as host modulatory therapy for the management of periodontitis. These host-modulating agents used as an adjunct tip the balance between periodontal health and disease progression in the direction of a healing response. In this article the host-modulating role of various systemically and locally delivered perioceutic agents will be reviewed.

Two dimensional alveolar ridge augmentation using particulate hydroxyapatite and collagen membrane: A case report.

BACKGROUND: Ridge augmentation procedures require bone regeneration outside of the existing bony walls or housing and are therefore often considered to be the most challenging surgical procedures. The bony deficiencies can be managed with GBR techniques involving bone grafting material and membrane while vertical augmentation may require the use of space-creating support mechanisms. Non-degradable membranes have been used for ridge augmentation with encouraging results however; requirement of second surgery for its removal and associated infection on exposure may compromise the desired results. These problems can be overcome by employing resorbable collagen membranes. Different bone graft materials are also used in combination with resorbable membranes, for prevention of membrane collapse and maintenance of space, as they lack sufficient rigidity. Particulate hydroxyapatite bone graft may be better alternative, because it treats the underlying bone defect to restore the natural support of the tissue architecture. Moreover, its use avoids potential donor site complications associated with autogenous block grafts. METHOD: Patient described in this report presented with missing right maxillary incisor with ridge deficiency. A treatment approach involving localised ridge augmentation with particulate hydroxyapatite and collagen membrane was used. RESULT: Six month post-operative periapical radiograph demonstrated a significant vertical bone fill. CONCLUSION: The clinical and radiographic findings of the present case suggests that HA in conjunction with a resorbable collagen membrane may be an acceptable alternative to the autogenous block graft and non-resorbable membrane in the treatment of compromised alveolar ridge deficiencies.

Implant Maintenance: A Clinical Update.

Introduction. The differences in the supporting structure of the implant make them more susceptible to inflammation and bone loss when plaque accumulates as compared to the teeth. Therefore, a comprehensive maintenance protocol should be followed to ensure the longevity of the implant. Material and Method. A research to provide scientific evidence supporting the feasibility of various implant care methods was carried out using various online resources to retrieve relevant studies published since 1985. Results. The electronic search yielded 708 titles, out of which a total of 42 articles were considered appropriate and finally included for the preparation of this review article. Discussion. A typical maintenance visit for patients with dental implants should last 1 hour and should be scheduled every 3 months to evaluate any changes in their oral and general history. It is essential to have a proper instrument selection to prevent damage to the implant surface and trauma to the peri-implant tissues. Conclusion. As the number of patients opting for dental implants is increasing, it becomes increasingly essential to know the differences between natural teeth and implant care and accept the challenges of maintaining these restorations.

Periodontio-integrated implants: A revolutionary concept.

Though the fields of regenerative dentistry and tissue engineering have undergone significant advancements, yet its application to the field of implant-dentistry is lacking; in the sense that presently the implants are being placed with the aim of attaining osseointegration without giving consideration to the regeneration of periodontium around the implant. The following article reveals the clinical benefits of such periodontio-integrated implants and reviews the relevant scientific proofs. A comprehensive research to provide scientific evidence supporting the feasibility of periodontio-integrated implants was carried out using various online resources such as PubMed, Wiley-Blackwell, Elsevier etc., to retrieve studies published between 1980 and 2012 using the following key words: "implant," "tissue engineering," "periodontium," "osseo-integration," "osseoperception," "regeneration" (and their synonyms) and it was found that in the past three decades, several successful experiments have been conducted to devise "implant supported by the periodontium"that can maintain form, function and potential proprioceptive responses similar to a natural tooth. Based on these staunch evidences, the possibility of the future clinical use of such implant can be strongly stated which would revolutionize the implant dentistry and will be favored by the patients as well. However, further studies are required to validate the same.

Superior vena cava syndrome associated with right-to-left shunt through systemic-to-pulmonary venous collaterals.

Superior vena cava (SVC) obstruction is associated with the gradual development of venous collaterals. We present a rare form of systemic-to-pulmonary subpleural collateral pathway that developed in the bridging subpleural pulmonary veins in a 54-year-old woman with complete SVC obstruction. This uncommon collateral pathway represents a rare form of acquired right-to-left shunt due to previous pleural adhesions with an increased risk of stroke due to right-to-left venous shunting, which requires lifelong anticoagulation.

Low level laser therapy in the treatment of aphthous ulcer.

Recurrent aphthous stomatitis (RAS) is one of the most common and painful ulcerative lesions of the oral cavity, but until now no cure has been recognized for it. Two patients diagnosed with minor RAS were treated in a single sitting with low level laser therapy using 940-nm diode laser. The lesions healed completely within 3-4 days and a follow-up for 1 showed no recurrence in these patients. According to the results of this study, low level laser therapy can decrease the healing time, pain intensity, size, and recurrence of the lesion in patients with minor RAS, and hence can be considered the most appropriate treatment modality for minor RAS, with greatest clinical effectiveness.

Essentials of periodontal medicine in preventive medicine.

Influence of systemic disorders on periodontal diseases is well established. However, of growing interest is the effect of periodontal diseases on numerous systemic diseases or conditions like cardiovascular disease, cerebrovascular disease, diabetes, pre-term low birth weight babies, preeclampsia, respiratory infections and others including osteoporosis, cancer, rheumatoid arthritis, erectile dysfunction, Alzheimer's disease, gastrointestinal disease, prostatitis, renal diseases, which has also been scientifically validated. This side of the oral-systemic link has been termed Periodontal Medicine and is potentially of great public health significance, as periodontal disease is largely preventable and in many instances readily treatable, hence, providing many new opportunities for preventing and improving prognosis of several systemic pathologic conditions. This review article highlights the importance of prevention and treatment of periodontal diseases as an essential part of preventive medicine to circumvent its deleterious effects on general health.

Evaluation of stress patterns in bone around dental implant for different abutment angulations under axial and oblique loading: A finite element analysis.

INTRODUCTION: The replacement of missing anterior teeth presents peculiar challenges to the Prosthodontist. Implants are increasingly gaining favour for the same. The morphology of existing bone in the premaxilla often dictates that implants are placed at angles that are difficult to restore with conventional abutments. However, the angulated abutments might transfer unfavourable forces to the implant or bone, thereby compromising the prognosis of the treatment. Because, it is difficult to assess the generated forces clinically, a finite element analysis was chosen for the present study as it is useful tool in estimating stress distribution in the contact area of the implant with the bone. MATERIALS AND METHODS: In this study, the frontal region of the maxilla was modelled with a cortical layer 1.5 mm thick containing an inner cancellous core. The implant was cylindrical, round ended, with length 13 mm and diameter 4.1 mm. The abutment was modelled as 7 mm in height with a 5 degree occlusal taper. The different abutment angulations used were 0°, 10°, 15° and 20°. The amount of loads used were 100, 125, 150, 175 and 200 N axially, and 50 N in oblique direction, to approximate the kind of loads seen in clinical situations. RESULT: It was seen that, as the abutment angulation changes from 0° to 20° both the compressive as well as tensile stresses increased; but, it is within the tolerance limit of the bone. CONCLUSION: It seems reasonably safe to use angled abutments in anterior implant supported prostheses, in the maxillary arch.

Free gingival autograft for augmentation of keratinized tissue in apical to gingival recession - A case report.

BACKGROUND: The treatment of the mucogingival problem is one of the main objectives of the periodontal therapy. The insufficient or absent attached gingiva increases the risk of development of gingival recessions. METHOD: One patient with Miller class II gingival recession and small vestibule depth in the frontal mandibular region was selected for treatment. Autogenous free gingival grafts harvested from the palatal mucosa were used to gain the attached gingiva. RESULT: The initial healing completed in 2 weeks without complication. The augmentation of the attached gingival tissue using the free gingival graft technique led to gain of attached gingiva in the treated regions. CONCLUSION: The limitations of the apical mucosal flap displacement for preparation of recipient site in situations with inadequate vestibule depth and small alveolar bone height require a graft with small width. The result from the presented case report with application of the free gingival graft indicates that it could be applied when augmentation of the attached gingiva tissue.