RESUMEN
The effectiveness of several copper coordination complexes in the induction of sister-chromatid exchanges (SCE) in meristematic cells of Allium cepa roots was studied. A significant increase in the frequencies of SCE was observed when cells were exposed to different concentrations of copper complexes for 3 h. This effect, which is concentration dependent, seems to be related to the possibility of intercalative binding modes between the planar copper complexes and DNA.
Asunto(s)
Cobre/farmacología , Intercambio de Cromátides Hermanas/efectos de los fármacos , Allium/efectos de los fármacos , Allium/genética , Daño del ADN , Sustancias Intercalantes/farmacología , Relación Estructura-ActividadRESUMEN
A generalization of the molar-ratio method is proposed, which allows study of relatively weak complexes. The method is based on treatment of the data from a molar-ratio saturation curve. From the mathematical expression derived, the stoichiometry, degree of condensation and stability constant are easily evaluated. Graphical representations of the results can be used advantageously.