Effects of Gly-His-Lys-D-Ala Peptide on Skin Wound Regeneration Processes.

We evaluated the effects of the Gly-His-Lys-D-Ala peptide in a dose 0.5 µg/kg on skin wound regeneration in male Wistar rats (n=80) after initial surgical debridement when administered intracutaneously around the site of injury. Histological (severity of the inflammatory reaction, formation of granulation tissue, and epithelialization terms) and morphometric (number of fibroblastic cells, macrophages, granulocytes, and lymphocytes) studies were performed on autopsy specimens on days 3, 7, 10, and 30 of the experiment. Daily intracutaneous injection of the peptide resulted in an increase in the number of fibroblastic cells and macrophages, as well as in a decrease in the number of granulocytes against the background of active wound contraction on day 30 of the experiment. Thus, Gly-His-Lys-D-Ala alleviated the inflammatory reaction and promoted the regenerative processes.

[Local management of purulent wounds with wound dressings]. / Mestnaya terapiya gnoinykh ran ranevymi pokrytiyami.

Purulent-inflammatory lesions of skin and soft tissues account for 30-35% of causes of admission to surgical hospitals. In outpatient system, this value reaches 35-60%. Moreover, suppuration of wounds after elective surgery occurs in 2-5% of cases. Adequately selected drugs for local wound therapy can achieve localization of purulent process and avoid spread of infection. This review summarizes data on development, experimental and clinical testing of wound dressings over the past 10 years, presents classification options and analyzes their advantages and disadvantages. The authors emphasize higher efficacy of hydrophilic coatings, and coatings based on chitosan, collagen, methylcellulose and its derivatives are preferred. It is advisable to introduce a component with antimicrobial, anesthetic and hemostatic activity stimulating tissue regeneration into the matrix of wound dressing. The priority is introduction of antiseptic substances causing fewer side effects compared to antibiotics. Further research and development of new wound dressings is a priority in surgery considering changing landscape and development of resistance of microorganisms.

[The efficiency of antiadhesive means with "mezogel" having a barrier effect after dissection of the adhesions in patients with acute adhesive intestinal obstruction].

An antiadhesive absorbable means "Mezogel" produced by the firm "Lintex" (St. Petersburg) after intraperitoneal administration to animals in experiments was shown to reliably effectively reduce the degree of a recurrent adhesion process, and in 40% of the cases to completely prevent repeated formation of adhesions in the abdominal cavity. The use of "Mezogel" for intraperitoneal administration after dissection of adhesions in patients with acute adhesive intestinal obstruction results in relieving the course of the nearest and long term postoperative periods and reducing the number of recurrences, thus improving quality of life of the patients.

[Experience of laparoscopic cholecystectomy in calculous cholecystitis]. / Opyt laparoskopicheskoi kholetsistéktomii pri kal'kuleznom kholetsistite.

Laparoscopic cholecystectomy (LCE) was performed in 330 patients aged from 14 to 77 years; it was completed successfully in 308 (93.3%) patients. Conversion to laparotomy and standard cholecystectomy was carried out in 22 patients (6.7%) due chiefly (86.4%) to inflammatory disorders of infrahepatic area. 188 (61%) LCE were performed for chronic calculous cholecystitis, 120--for acute calculous cholecystitis. In the latter group destructive forms of cholecystitis were revealed in 34.2% patients. Combined with LCE interventions were performed in 13 patients (4.2%). There were no lethal outcomes after LCE. Severe intra- and postoperative complications occurred in 26 (8.4%) patients; laparotomy for removing these complications was necessary in 3 patients (1%).

[The experimental grounds and clinical application of domestic endoprosthesis "Esfil" for plasty of the abdominal wall]. / Eksperimental'noe obosnovanie i klinicheskoe primenenie otechestvennogo éndoproteza "Esfil" dlia plastiki briushnoi stenki.

An investigation of physico-mechanical properties and a histological examination of meshed endoprostheses "Esfil" ("Lintex" St.Petersburg) and nets "Prolene" ("Ethicon") implanted into the abdominal wall of 66 Chinchilla rabbits was carried out under experimental conditions. The comparative experimental investigation has shown that both materials possess sufficient physico-mechanical properties to resist intra-abdominal pressure and when implanted they cause an inconsiderable inflammatory reaction followed by the formation of a solid connective tissue capsule. The endoprosthesis "Esfil" was used for plasty of the abdominal wall in 51 patients with postoperative ventral hernias. The endoprosthesis "Esfil" was found to meet all the requirements made to endoprostheses for plasty of the abdominal wall. Its use in clinical practice allows the restoration of quality of life of such patients.