RESUMEN
Campylobacter jejuni is one of the most intriguing human foodborne bacterial pathogen. Its survival throughout the food processing chain and its pathogenesis mechanisms in humans remain enigmatic. Living in the animal guts and particularly in avian intestine as a commensal bacterium, this microorganism is frequently isolated from meat products. Ultra high pressure (HP) is a promising alternative to thermal technology for microbial safety of foodstuffs with less organoleptic and nutritional alterations. Its application could be extended to meat products potentially contaminated by C. jejuni. To evaluate the response of Campylobacter to this technological stress and subsequent recovery at a molecular level, a dynamic 2-DE-based proteomic approach has been implemented. After cultivation, C. jejuni cells were conditioned in a high-pressure chamber and transferred to fresh medium for recovery. The protein abundance dynamics at the proteome scale were analyzed by 2-DE during the cellular process of cell injury and recovery. Monitoring protein abundance through time unraveled the basic metabolisms involved in this cellular process. The significance of the proteome evolution modulated by HP and subsequent recovery is discussed in the context of a specific cellular response to stress and recovery of C. jejuni with 69 spots showing significant changes through time.
Asunto(s)
Campylobacter jejuni/metabolismo , Proteómica/métodos , Animales , Bioquímica/métodos , Pollos/microbiología , Citoplasma/metabolismo , Electroforesis en Gel Bidimensional/métodos , Microbiología de Alimentos , Regulación Bacteriana de la Expresión Génica , Concentración de Iones de Hidrógeno , Modelos Biológicos , Estrés Oxidativo , Presión , Proteoma/genética , Cloruro de Sodio/química , Factores de TiempoRESUMEN
Campylobacteriosis has been recognized as the major bacterial food-borne infection worldwide. Campylobacter, especially C. jejuni, contaminate mainly poultry meat. Although more sensitive than other food-borne pathogens to many stresses, C. jejuni can survive food processing and go on to reach its final reservoir (the human gut). Genomic analyses of this organism indicate a lack of genes described in other gram-negative bacteria to overcome stresses. The high-pressure recovery response of C. jejuni 81-176 was analyzed from two-dimensional electrophoretic profiles of the cytoplasmic proteome. The main cellular mechanisms controlling the down- and upregulated proteins are discussed.