A comparison of gelatine surrogates for wound track assessment.

The use of ordnance gelatine has been widespread in the field of ballistics as a simulant for soft tissue when assessing ballistic threats. However, the traditional method of preparing ordnance gelatine is time-consuming and requires precision to ensure that the final mold meets the required specifications. Furthermore, temperature control is necessary post-production, and there are limitations on its usage duration. To address these issues, manufacturers have developed pre-mixed, gelatine-like products that are stable at room temperature and require less preparation time. Nonetheless, it is uncertain whether these new products can perform in the same manner as the gold standard of ordnance gelatine. This study used five types of blocks, including ordnance gelatine (10% and 20%), Clear Ballistics (10% and 20%) and Perma-Gel (10%) and subjected them to 9 mm, 0.380 Auto fired from a universal receiver and a 5.56 × 45 mm ammunition fired by a certified firearms instructor. Delta-V and total energy dissipation were measured after each test using data collected from ballistic chronographs placed in front of and behind each block. High-speed video was recorded, and a cut-down analysis conducted. The findings revealed variations in energy dissipation and fissure formation within the block, with greater energy based on fissure formation observed in the ordnance gelatine. Additionally, the high-speed video showed the occurrence of secondary combustions occurring in the premixed gelatines.

Intravenously administered nanoparticles increase survival following blast trauma.

Explosions account for 79% of combat-related injuries, leading to multiorgan hemorrhage and uncontrolled bleeding. Uncontrolled bleeding is the leading cause of death in battlefield traumas as well as in civilian life. We need to stop the bleeding quickly to save lives, but, shockingly, there are no treatments to stop internal bleeding. A therapy that halts bleeding in a site-specific manner and is safe, stable at room temperature, and easily administered is critical for the advancement of trauma care. To address this need, we have developed hemostatic nanoparticles that are administered intravenously. When tested in a model of blast trauma with multiorgan hemorrhaging, i.v. administration of the hemostatic nanoparticles led to a significant improvement in survival over the short term (1 h postblast). No complications from this treatment were apparent out to 3 wk. This work demonstrates that these particles have the potential to save lives and fundamentally change trauma care.

Development of the NOCSAE Standard to Reduce the Risk of Commotio Cordis.

BACKGROUND: Commotio cordis, sudden cardiac death (SCD) caused by relatively innocent impact to the chest, is one of the leading causes of SCD in sports. Commercial chest protectors have not been demonstrated to mitigate the risk of these SCDs. METHODS: To develop a standard to assess chest protectors, 4 phases occurred. A physiological commotio cordis model was utilized to assess variables that predicted for SCD. Next, a surrogate model was developed based on data from the physiological model, and the attenuation in risk was assessed. In the third phase, this model was calibrated and validated. Finally, National Operating Committee on Standards for Athletic Equipment adopted the standard and had an open review process with revision of the standard over 3 years. RESULTS: Of all variables, impact force was the most robust at predicting SCD. Chest wall protectors which could reduce the force of impact to under thresholds were predicted to reduce the risk of SCD. The correlation between the experimental model and the mechanical surrogate ranged from 0.783 with a lacrosse ball at 30 mph to 0.898 with a baseball at 50 mph. The standard was licensed to National Operating Committee on Standards for Athletic Equipment which initially adopted the standard in January 2018, and finalized in July 2021. CONCLUSIONS: An effective mechanical surrogate based on physiological data from a well-established model of commotio cordis predicts the reduction in SCD with chest protectors. A greater reduction in force provides a great degree of protection from commotio cordis. This new National Operating Committee on Standards for Athletic Equipment standard for chest protectors should result in a significant reduction in the risk of commotio cordis on the playing field.

Biofidelity and Limitations of Instrumented Mouthguard Systems for Assessment of Rigid Body Head Kinematics.

Instrumented mouthguard systems (iMGs) are commonly used to study rigid body head kinematics across a variety of athletic environments. Previous work has found good fidelity for iMGs rigidly fixed to anthropomorphic test device (ATD) headforms when compared to reference systems, but few validation studies have focused on iMG performance in human cadaver heads. Here, we examine the performance of two boil-and-bite style iMGs in helmeted cadaver heads. Three unembalmed human cadaver heads were fitted with two instrumented boil-and-bite mouthguards [Prevent Biometrics and Diversified Technical Systems (DTS)] per manufacturer instructions. Reference sensors were rigidly fixed to each specimen. Specimens were fitted with a Riddell SpeedFlex American football helmet and impacted with a rigid impactor at three velocities and locations. All impact kinematics were compared at the head center of gravity. The Prevent iMG performed comparably to the reference system up to ~ 60 g in linear acceleration, but overall had poor correlation (CCC = 0.39). Prevent iMG angular velocity and BrIC generally well correlated with the reference, while underestimating HIC and overestimating HIC duration. The DTS iMG consistently overestimated the reference across all measures, with linear acceleration error ranging from 10 to 66%, and angular acceleration errors greater than 300%. Neither iMG demonstrated consistent agreement with the reference system. While iMG validation efforts have utilized ATD testing, this study highlights the need for cadaver testing and validation of devices intended for use in-vivo, particularly when considering realistic (non-idealized) sensor-skull coupling, when accounting for interactions with the mandible and when subject-specific anatomy may affect device performance.

Assessment of a Perfusion and Ventilation Method for Detecting Lung and Liver Injury in a Cadaveric Model.

Surgical simulation models have been developed using post-mortem human subjects (PMHS). These models involve the pressurization and ventilation of the PMHS to create a more realistic environment for training and the practice of surgical procedures. The overall objective of this study was to determine the feasibility of a previously developed surgical simulation model to detect soft tissue injuries during a ballistic impact to the torso. One of the main limitations of using PMHS for the assessment of soft tissue injuries in the field of injury biomechanics is the lack of physiological blood flow. To overcome this limitation, the assessment of the surgical simulation model for use in injury biomechanics applications was conducted based on data collected from behind armor blunt trauma (BABT) case studies. Documented injuries in real-world cases included anterior lung contusion, posterior lung contusion, and liver contusion. These real-world injuries were compared to those seen post-impact in the PMHS using pathological and histological techniques. Discussion of limitations and future work is presented.

Longitudinal, prospective study of head impacts in male high school football players.

INTRODUCTION: Repetitive, subconcussive events may adversely affect the brain and cognition during sensitive periods of development. Prevention of neurocognitive consequences of concussion in high school football is therefore an important public health priority. We aimed to identify the player positions and demographic, behavioral, cognitive, and impact characteristics that predict the frequency and acceleration of head impacts in high school football players. METHODS: In this prospective study, three cohorts of adolescent male athletes (N = 53, 28.3% Hispanic) were recruited over three successive seasons in a high school American football program. Demographic and cognitive functioning were assessed at baseline prior to participating in football. Helmet sensors recorded impact frequency and acceleration. Each head impact was captured on film from five different angles. Research staff verified and characterized on-field impacts. Player-level Poisson regressions and year-level and impact-level linear mixed-effect models were used to determine demographic, behavioral, cognitive, and impact characteristics as predictors of impact frequency and acceleration. RESULTS: 4,678 valid impacts were recorded. Impact frequency positively associated with baseline symptoms of hyperactivity-impulsivity [ß(SE) = 1.05 impacts per year per unit of symptom severity (1.00), p = 0.01] and inattentiveness [ß(SE) = 1.003 impacts per year per T-score unit (1.001), p = 0.01]. Compared to quarterbacks, the highest acceleration impacts were sustained by kickers/punters [ß(SE) = 21.5 g's higher (7.1), p = 0.002], kick/punt returners [ß(SE) = 9.3 g's higher (4.4), p = 0.03], and defensive backs [ß(SE) = 4.9 g's higher (2.5), p = 0.05]. Impacts were more frequent in the second [ß(SE) = 33.4 impacts (14.2), p = 0.02)] and third [ß(SE) = 50.9 impacts (20.1), p = 0.01] year of play. Acceleration was highest in top-of-the-head impacts [ß(SE) = 4.4 g's higher (0.8), p<0.001]. CONCLUSION: Including screening questions for Attention-Deficit/Hyperactivity Disorder in pre-participation evaluations can help identify a subset of prospective football players who may be at risk for increased head impacts. Position-specific strategies to modify kickoffs and correct tackling and blocking may also reduce impact burden.

Blast-induced neurotrauma leads to neurochemical changes and neuronal degeneration in the rat hippocampus.

Blast-induced neurotrauma is a major concern because of the complex expression of neuropsychiatric disorders after exposure. Disruptions in neuronal function, proximal in time to blast exposure, may eventually contribute to the late emergence of clinical deficits. Using magic angle spinning ¹H MRS and a rodent model of blast-induced neurotrauma, we found acute (24-48 h) decreases in succinate, glutathione, glutamate, phosphorylethanolamine and γ-aminobutyric acid, no change in N-acetylaspartate and increased glycerophosphorylcholine, alterations consistent with mitochondrial distress, altered neurochemical transmission and increased membrane turnover. Increased levels of the apoptotic markers Bax and caspase-3 suggested active cell death, consistent with increased FluoroJade B staining in the hippocampus. Elevated levels of glial fibrillary acidic protein suggested ongoing inflammation without diffuse axonal injury measured by no change in ß-amyloid precursor protein. In conclusion, blast-induced neurotrauma induces a metabolic cascade associated with neuronal loss in the hippocampus in the acute period following exposure.

Biomechanical comparison of internal fixation techniques for the Akin osteotomy of the proximal phalanx.

The Akin osteotomy is performed at the proximal phalanx for correction of an abducted great toe in a hallux abducto valgus deformity. Several internal fixation techniques have been widely advocated; however, their respective stabilities have not been compared. A biomechanical analysis was performed comparing 5 commonly used fixation techniques for the Akin osteotomy to determine the strongest method in simulated weightbearing in sawbone models. An Akin osteotomy was uniformly performed on 25 sawbones and fixated with 5 different internal fixation types, including a 2-hole locking plate and locking screws, a heat-sensitive memory staple (8 mm × 8 mm), a 28-gauge monofilament wire, 2.7-mm bicortical screws, and crossed 0.062-in. Kirschner wires. The results of simulated weightbearing load to failure rates with an Instron compression device demonstrated the following mean load to failures: crossed Kirschner wire, 57.05 N; 2-hole locking plate, 36.49 N; monofilament wire, 35.69 N; heat-sensitive memory staple, 34.32 N; and 2.7-mm bicortical screw, 13.66 N. Statistical analysis demonstrated the crossed Kirschner wire technique performed significantly better than the other fixation techniques (p < .007); the 4 other techniques were found not to be significantly different statistically (p = .041) from each another. Our study results suggest a crossing Kirschner wire construct significantly increases the stability of the Akin osteotomy in a sawbone model. This might be clinically extrapolated in an effort to improve patient outcomes because these osteotomies can undergo nonunion and malunion, resulting in postoperative pain and swelling.

Ballistic fractures: indirect fracture to bone.

BACKGROUND: Two mechanisms of injury, the temporary cavity and the sonic wave, have been proposed to produce indirect fractures as a projectile passes nearby in tissue. The purpose of this study is to evaluate the temporal relationship of pressure waves using strain gauge technology and high-speed video to elucidate whether the sonic wave, the temporary cavity, or both are responsible for the formation of indirect fractures. METHODS: Twenty-eight fresh frozen cadaveric diaphyseal tibia (2) and femurs (26) were implanted into ordnance gelatin blocks. Shots were fired using 9- and 5.56-mm bullets traversing through the gelatin only, passing close to the edge of the bone, but not touching, to produce an indirect fracture. High-speed video of the impact event was collected at 20,000 frames/s. Acquisition of the strain data were synchronized with the video at 20,000 Hz. The exact time of fracture was determined by analyzing and comparing the strain gauge output and video. RESULTS: Twenty-eight shots were fired, 2 with 9-mm bullets and 26 with 5.56-mm bullets. Eight indirect fractures that occurred were of a simple (oblique or wedge) pattern. Comparison of the average distance of the projectile from the bone was 9.68 mm (range, 3-20 mm) for fractured specimens and 15.15 mm (range, 7-28 mm) for nonfractured specimens (Student's t test, p = 0.036). CONCLUSIONS: In this study, indirect fractures were produced after passage of the projectile. Thus, the temporary cavity, not the sonic wave, was responsible for the indirect fractures.

Incidence of law enforcement officer fatalities related to motor vehicle accidents.

A recent FBI report indicates that motor vehicle accidents (MVA) account for 71% of accidental law enforcement officer (LEO) fatalities. To better understand the factors surrounding these fatalities, a review of cases reported to the Officer Down Memorial Page (ODMP) was conducted. A total of 738 MVA fatalities over ten years were included in this analysis. When comparing younger (<34 years) to older officers (≥ 35 years), there was a statistically significant difference with more younger officers reported to have been going at a high rate of speed at the time of the accident (p = 0.011) and were involved more often in single car accidents (p = 0.0207). The data also indicated a fatality rate of 5.4 fatalities per 100,000 officers annually; twice that of other professionals involving high speed operations. Even given the downward trend of MVA fatalities, policy changes and training to help reduce these number of fatalities is warranted.

Dynamic Response of the Thoracolumbar and Sacral Spine to Simulated Underbody Blast Loading in Whole Body Post Mortem Human Subject Tests.

Fourteen simulated underbody blast impact sled tests were performed using a horizontal deceleration sled with the aim of evaluating the dynamic response of the spine in under various conditions. Conditions were characterized by input (peak velocity and time-to-peak velocity for the seat and floor), seat type (rigid or padded) and the presence of personnel protective equipment (PPE). A 50% (T12) and 30% (T8) reduction in the thoracic spine response for the specimens outfitted with PPE was observed. Longer duration seat pulses (55 ms) resulted in a 68-78% reduction in the magnitude of spine responses and a reduction in the injuries at the pelvis, thoracic and lumbar regions when compared to shorter seat pulses (10 ms). The trend analysis for the peak Z (caudal to cranial) acceleration measured along the spine showed a quadratic fit (p < 0.05), rejecting the hypothesis that the magnitude of the acceleration would decrease linearly as the load traveled caudal to cranial through the spine during an Underbody Blast (UBB) event. A UBB event occurs when an explosion beneath a vehicle propels the vehicle and its occupants vertically. Further analysis revealed a relationship (p < 0.01) between peak sacrum acceleration and peak spine accelerations measured at all levels. This study provides an initial analysis of the relationship between input conditions and spine response in a simulated underbody blast environment.

Comparison of pullout strength between 3.5-mm fully threaded, bicortical screws and 4.0-mm partially threaded, cancellous screws in the fixation of medial malleolar fractures.

Displaced medial malleolus fractures are considered unstable and typically require open reduction and internal fixation for anatomic reduction and early joint range of motion. These fractures are usually fixated with either compression lag screws or tension band wiring depending on the fracture pattern, size of the distal fragment, and bone quality. When fracture fixation fails, it is typically in pullout strength. Failure of primary bone healing can result in nonunion, malunion, and need for revision surgery. The current study wished to explore a potentially stronger fixation technique in regard to pullout strength for medial malleolar fractures compared with traditional cancellous screws. This was a comparative study of the relative pullout strength of 2 fully threaded 3.5-mm bicortical screws versus 2 partially threaded 4.0-mm cancellous screws for the fixation of medial malleolar fractures. Ten fresh-frozen limbs from 5 cadavers, mean age 79 years (range of 65-97 years), were tested using the Instron 8500 Plus system. The median force recorded at 2 mm of distraction using unicortical partially threaded cancellous screws was 116.2 N (range 70.2 to 355.5N) compared with 327.6 N (range 117.5 to 804.3 N) in the fully threaded bicortical screw (P = .04). The unicortical screw fixation displayed only 64.53% of the median strength noted with the bicortical screw fixation at clinical failure. The current study demonstrated statistically significantly greater pullout strength for 3.5-mm bicortical screws when compared with 4.0-mm partially threaded cancellous screws used to fixate medial malleolar fractures in a cadaveric model.

Development of biomechanical response corridors of the head to blunt ballistic temporo-parietal impact.

There is a need to study the biomechanical response of the head to blunt ballistic impact. While the frequency of less-lethal munition impacts to the head may be less than other vital body regions, more serious injuries have been attributed to these impacts. This study aims to establish biomechanical response corridors for the temporo-parietal region for future development of biomechanical surrogate devices. Seven unembalmed post-mortem human subject specimens were exposed to blunt ballistic temporo-parietal head impact (103 g, 38 mm diameter impactor) to determine the force-time, deformation-time, and force-deformation responses. Comparisons were made to responses from prior blunt ballistic head impact studies, as well as automotive-related impact studies. Peak forces for impact condition A (19.5+/-2.6 m/s) were 3659+/-1248 N with deformations at peak force of 7.3+/-2.1 mm. Peak forces for impact condition B (33.6+/-1.4 m/s) were 5809+/-1874 N with deformations at peak force of 9.9+/-2.6 mm. Seven fractures were produced in the seven specimens. Depressed comminuted fracture types were documented in six of the seven cases. The average stiffness of the temporo-parietal region under blunt ballistic impact was 0.46+/-0.14 kN/mm. Stiffness results indicate that the response of the temporo-parietal region is similar to the forehead under blunt ballistic loading conditions. In addition, the response is significantly less stiff when compared with temporo-parietal impacts performed in automotive-related studies. These data provide the foundation for future research in the area of blunt ballistic head impact research including the development of biomechanical surrogates and computational models.

Force, reaction time, and precision of Kung Fu strikes.

The goal was to compare values of force, precision, and reaction time of several martial arts punches and palm strikes performed by advanced and intermediate Kung Fu practitioners, both men and women. 13 Kung Fu practitioners, 10 men and three women, participated. Only the men, three advanced and seven intermediate, were considered for comparisons between levels. Reaction time values were obtained using two high speed cameras that recorded each strike at 2500 Hz. Force of impact was measured by a load cell. For comparisons of groups, force data were normalized by participant's body mass and height. Precision of the strikes was determined by a high speed pressure sensor. The results show that palm strikes were stronger than punches. Women in the study presented, on average, lower values of reaction time and force but higher values of precision than men. Advanced participants presented higher forces than intermediate participants. Significant negative correlations between the values of force and precision and the values of force and reaction time were also found.

The effects of height and distance on the force production and acceleration in martial arts strikes.

Almost all cultures have roots in some sort of self defence system and yet there is relatively little research in this area, outside of a sports related environment. This project investigated different applications of strikes from Kung Fu practitioners that have not been addressed before in the literature. Punch and palm strikes were directly compared from different heights and distances, with the use of a load cell, accelerometers, and high speed video. The data indicated that the arm accelerations of both strikes were similar, although the force and resulting acceleration of the target were significantly greater for the palm strikes. Additionally, the relative height at which the strike was delivered was also investigated. The overall conclusion is that the palm strike is a more effective strike for transferring force to an object. It can also be concluded that an attack to the chest would be ideal for maximizing impact force and moving an opponent off balance. Key PointsIt has been determined that the palm strike is more effective than the punch for developing force and for transferring momentum, most likely the result of a reduced number of rigid links and joints.A strike at head level is less effective than a strike at chest level for developing force and transferring momentum.Distance plays an effect on the overall force and momentum changes, and most likely is dependent on the velocity of the limb and alignment of the bones prior to impact.The teaching of self defence for novices and law enforcement would benefit from including the palm strike as a high priority technique.

Biomechanical response of the human mandible to impacts of the chin.

The purpose of this study was to determine the force-time and force-displacement response of the human mandible under direct loading at the chin. Sub-fracture response of the mandible and temporomandibular joint (TMJ) were analyzed from 10 cadavers that were impacted at the chin with a 2.8-kg mass at drop heights of 300, 400 and 500 mm and a 5.2-kg mass at 500 mm. Motion of radio-opaque markers adhered to the surface of the bone was recorded at 1000 Hz by a bi-planar X-ray and converted to three-dimensional coordinates. Peak force ranged from 0.90 to 4.54 kN causing chin displacement of 1.2-4.4 mm. A bi-linear response was observed with stiffness of 475.1+/-199.8 kN/m for chin displacement resulting from loading up to 0.6 kN and 2381.6+/-495.7 kN/m for loads from 0.6 to 3.25 kN. This defines the biomechanical response of the mandible for chin motion under impact loading. The response of different segments of the mandible and TMJ are also documented. Force-time and force-displacement response corridors for the mandible can be used for finite element model and/or the development and validation of a biomechanical surrogate.

Validation of lower limb surrogates as injury assessment tools in floor impacts due to anti-vehicular land mines.

The aim of this study was to assess the ability of lower limb surrogates to predict injury due to floor/foot plate impact in military vehicles during anti-vehicular land mine explosions. Testing was conducted using two loading conditions simulated to represent those conditions created in the field. The lower condition was represented by a 24-kg mass impactor with a velocity of 4.7 m/s. The higher loading condition was represented by a 37-kg mass impactor with a velocity of 8.3 m/s. Two biomechanical surrogates were evaluated using the loading conditions: 50th percentile Hybrid III foot/ankle and Test Device for Human Occupant Restraint THOR-Lx. Comparisons of the force-time response were made to established corridors. Results show a better correlation to the corridors with the THOR-Lx; however, future improvements to the THOR-Lx are recommended.

Size as an Important Determinant of Chest Blow-induced Commotio Cordis.

PURPOSE: Commotio cordis is sudden cardiac death caused by a relatively innocent blow to the left chest wall. Adolescents account for the majority of the cases; whether this is due to the higher frequency of adolescents playing ball sports or whether there is some maturational reduction of risk is not known. METHODS: In a swine model of commotio cordis, the effect of body weight/size (directly related to age) to the susceptibility of chest impact-induced ventricular fibrillation (VF) is examined. METHODS: Ball impacts were delivered at escalating velocities from 48.3 to 96.9 km·h (30-60 mph) to 128 swine ranging in weight from 5 to 54 kg. RESULTS: VF occurred in 29% of impacts to the smallest animals compared with 34% in the 14- to 239-kg group, 27% in the 24- to 33.9-kg group, 30% in 34- to 43-kg group, and 15% in the 44- to 54-kg animals. The highest-weight group was associated with a significantly lower incidence of VF compared with other weights (P = 0.002). In a multivariate logistic regression analysis, controlling for repeated measures, four variables predicted VF: body weight (P = 0.0008), velocity (P < 0.0001), distance from the center of the heart, (P < 0.0001), and peak left ventricular pressure induced by the blow (P = 0.0007). CONCLUSIONS: In this experimental model, animals weighing <44 kg seem to have a similar susceptibility to commotio cordis, whereas animals weighing ≥44 kg have a lower susceptibility. An increase in size of the individual, rather than reduced play of ball sports, is the likely reason for the decreased commotio cordis incidence in older individuals.

A Molecular Method to Detect Wound Cells in Bloodstains Resultant of Sharp Force Injuries for Crime Scene Reconstruction.

Previous research by the authors on an animal model showed that bloodstains can contain additional information about their somatic origin in the form of wound cells. Bloodstains produced by a gunshot wound to the head were distinguished from bloodstains produced by a gunshot wound to the chest by testing the stains for a brain microRNA marker. In this study, the effectiveness of the technique was examined on blood drops shed externally from a stab wound to the liver of rat carcasses. Specifically, investigations were conducted on the liver microRNA marker, rno-mir-122-3p, with the QIAGEN miScript System, and PCR analysis. Between the two stabbing methods used, 67% of the scalpel blades and 57% of the blood drops tested positive for rno-mir-122-3p; however, other samples tested negative giving inconclusive results as to the wound-of-origin. The amount of the liver cells in the bloodstains appeared to be related to the extent of trauma.

The physiologic effects of a conducted electrical weapon in swine.

STUDY OBJECTIVE: By using an animal model, we determine whether repeated exposures to a conducted electrical weapon could have physiologic consequences. METHODS: Exposures to the Stinger S-400 conducted electrical weapon were applied to 10 healthy, anesthetized, Yorkshire-cross, male swine by attaching probes from the cartridge to the sternal notch and anterolateral thorax at a distance of 21.5 cm. The standard pulse generated by the Stinger S-400 during the normal application was applied 20 times during 31 minutes. To evaluate the health effects of the exposures, key physiologic characteristics were evaluated, including arterial pH, PCO2, PO2, blood lactate, cardiac output, ECG, pulse rate, mean arterial pressure, central venous pressure, pulmonary artery pressure and airway pressure, and the cardiac marker troponin I. RESULTS: There were notable changes in pH, PCO2, blood lactate, cardiac output, and mean arterial pressure after 1 or more sets of exposures, all of which normalized during the next few hours. Troponin I, PO2, pulse rate, mean arterial pressure, central venous pressure, pulmonary artery pressure, and airway pressure did not change markedly during or after the shocks. Three premature ventricular contractions occurred in one animal; all other ECG results were normal. CONCLUSION: Repeated exposures to a conducted electrical weapon result in respiratory acidosis, metabolic vasodilation, and an increase in blood lactate level. These effects were transient in this study, with full recovery by 4 hours postexposure. The Stinger S-400 appears to have no serious adverse physiologic effects on healthy, anesthetized swine.