RESUMEN
The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17ß-benzoate (E(2)) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200âmg), daidzein (50âmg), or E(2) (0.4âmg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E(2) improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5âmg/cm(3) in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4âN in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. Serum daidzein was 54±6âng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E(2) compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBCAsunto(s)
Huesos/efectos de los fármacos
, Alcanfor/análogos & derivados
, Estrógenos/farmacología
, Isoflavonas/farmacología
, Fosfatasa Ácida/genética
, Fosfatasa Alcalina/sangre
, Fosfatasa Alcalina/genética
, Animales
, Fenómenos Biomecánicos
, Densidad Ósea/efectos de los fármacos
, Enfermedades Óseas Metabólicas/fisiopatología
, Huesos/metabolismo
, Huesos/cirugía
, Callo Óseo/efectos de los fármacos
, Callo Óseo/metabolismo
, Alcanfor/administración & dosificación
, Alcanfor/farmacología
, Dieta
, Estrógenos/administración & dosificación
, Femenino
, Expresión Génica/efectos de los fármacos
, Factor I del Crecimiento Similar a la Insulina/genética
, Isoenzimas/genética
, Isoflavonas/administración & dosificación
, Vértebras Lumbares/efectos de los fármacos
, Vértebras Lumbares/patología
, Vértebras Lumbares/fisiopatología
, Osteocalcina/sangre
, Osteocalcina/genética
, Osteotomía
, Ratas
, Ratas Sprague-Dawley
, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
, Fosfatasa Ácida Tartratorresistente
, Tibia/efectos de los fármacos
, Tibia/patología
, Tibia/fisiopatología
, Tomografía Computarizada por Rayos X/métodos