RESUMEN
OBJECTIVES: The Sterilization Unit of the Narbonne Hospital Center (France) has decided to embark on a process of NF EN ISO 9001: 2015 certification. The objective is to describe how the working group has appropriated the provisions relating to staff training in order to build a skills development plan for its Sterilization agents. METHODS: A multi-professional working group has been set up. After a preliminary inventory, an inventory of skills needs, expectations of the agents, available means, and a bibliographical research, the group drew up a training plan with the support of a quality engineer from the Hospital Centre. The training plan was validated by a review of the management of the establishment. RESULTS: Several teaching aids were chosen: a serious game developed by the working group, the planning of instrumentation sessions, quality meetings and feedback committees. The principle of transdisciplinarity and recourse to multi-professional exchanges is the common thread in the elaboration of the training plan. CONCLUSION: The use of the selected materials is formalised in the form of a skills development plan indexed in the institution's quality management system. The application of the requirements of the ISO 9001 standard in terms of training in our Sterilization quality management system enables risk control and continuous improvement of the training plan to comply with technical and regulatory changes in the profession.
Asunto(s)
Certificación , Esterilización , Francia , Hospitales , HumanosRESUMEN
OBJECTIVE: To review the embryology, the clinical findings and the appearance in multidetector computed tomography (MDCT) of complicated Meckel's diverticulum (DM) in adults. CONCLUSION: The presence of an MD in a MDCT should be suspected when a blind tubular structure connected to the terminal ileum by a neck of variable size is observed. Multiplanar 2D reconstructions images and CT enterography can help in the diagnosis. In patients with symptoms of obstruction or intestinal inflammation, the possibility of a complication secondary to a MD should be considered, especially when the terminal ileum and the vermiform appendix are normal. MDCT angiography, in cases of acute gastrointestinal bleeding, can identify contrast extravasation and accumulation of blood in the MD, and could demonstrate the persistent omphalomesenteric artery, which is diagnostic of MD. Other MDCT findings such as the presence of enteroliths or associated inflammatory changes around the MD can facilitate the preoperative diagnosis of MD complications.
Asunto(s)
Divertículo Ileal/diagnóstico por imagen , Tomografía Computarizada Multidetector , Adulto , Anciano , Femenino , Humanos , Masculino , Divertículo Ileal/complicaciones , Persona de Mediana Edad , Adulto JovenRESUMEN
Some case reports have been published in the literature about thrombosis associated with several risk factors, especially in hypercoagulability state. Nevertheless, we have not found any description of a case like this of hyperthrombophilia status without thrombotic events. We present a 75 year old woman who was assessed for thrombophilia state on occasion of a deep venous thrombosis which affected to her daughter. Many thrombosis risk factors were detected (13 altogether), both inherited and acquired, arterial and venous, that surprisingly, have not led to any thrombosis event. Its description led us to highlight the risk factors of this patient and to open questions about the present knowledge on etiology and etiopathogenesis of thrombotic phenomena.
Asunto(s)
Trombofilia/fisiopatología , Anciano , Femenino , Humanos , Factores de Riesgo , Trombosis/etiologíaRESUMEN
Cytokeratin K19 (CK19) expression was evaluated by a reverse transcription PCR method (RT-PCR) in the RNA obtained from peripheral blood stem cell collections (PBSC) from four patients with breast cancers (BC) and 34 mononucleated blood cell (MBC) negative controls (17 PBMC from normal subjects 12 PBSC from different types of leukaemias--M3, M4Eo, M2, etc.--and two from patients with Hodgkin's lymphoma; and three bone marrow (BM) collections). Two BC tissues were taken as positive controls. The method studied (Datta YH, Paul T, Adams PT, Drobyski WR. Sensitive detection of occult breast cancer by reverse transcription polymerase chain reaction. J Oncol 1994;12:475-8) is sensitive enough to allow the detection of CK19 transcripts in a 10(-6) dilution of cDNA reverse transcribed from 1 microgram of BC RNA, but CK19 transcripts were also detected in 64% of the RNA obtained from the MBC controls. However, the amplified product detected in the control samples represents the transcript of the CK19 gene as confirmed by the results of Mae III digestion. It should be pointed out that although the CK19 expression was detected, the levels of expression in PBMC were almost negligible for they disappeared at 1:5 cDNA dilution. Moreover, a direct relationship between the number of BC cells added to PBMC and the increasing dilution levels of the cDNA necessary to prevent CK19 expression was observed. This allows us to conclude that the cDNA dilutions make it possible to distinguish the false from the true positive samples and that, in addition, the cDNA dilutions inform about the degree of BC cell contamination.
Asunto(s)
Expresión Génica , Queratinas/genética , Leucocitos Mononucleares/metabolismo , Reacción en Cadena de la Polimerasa/métodos , Neoplasias de la Mama/genética , Estudios de Casos y Controles , ADN Complementario/sangre , ADN Complementario/genética , ADN de Neoplasias/sangre , ADN de Neoplasias/genética , Estudios de Evaluación como Asunto , Femenino , Células Madre Hematopoyéticas/metabolismo , Enfermedad de Hodgkin/genética , Humanos , Leucemia/genética , Reacción en Cadena de la Polimerasa/estadística & datos numéricos , Sensibilidad y EspecificidadRESUMEN
Living donors of femoral heads belong to a highly specific group in terms of age and pathological characteristic, forming the core of the group who supply many tissue banks with spongy bone, to cover the needs of several specialties in the field of repair and corrective surgery. Analysis for the presence of the hepatitis C virus in this population was conducted as a part of quality control programs, while habitual blood donors from the same geographical location were used as the control group. Although the comparison of results could give rise to erroneous interpretations, due to the lack of qualitative fit between the groups, a much higher incidence of infection by VHC was found in the donors of spongy bone than was the case for blood donors (3.816% vs. 0.569%), although a smaller proportion of donors had hepatic alterations (measured by transaminases). In spite of the fact that the prevalence of VHC is almost 6 times that corresponding to the control group, the elimination of live donors of spongy bone would create serious problems with supply to tissue banks. We therefore propose that more severe exclusion criteria be applied to the selection of bone donors, and also that sterilisation techniques be employed, using physical-chemical procedures (liophilisation, dehydration, chemical treatment, irradiation) to process these tissues. We also recommend that younger multiple organ tissue donors be used as sources of spongy bone for cold storage that is not to be subjected to any additional sterilisation treatment.
Asunto(s)
Hepatitis C/epidemiología , Bancos de Tejidos , Donantes de Tejidos , Adulto , Anciano , Femenino , Cabeza Femoral/trasplante , Hepacivirus , Hepatitis C/transmisión , Hepatitis C/virología , Humanos , Masculino , Persona de Mediana EdadAsunto(s)
Enfermedades Fetales/prevención & control , Hepatitis B Crónica/prevención & control , Inmunoglobulina G/uso terapéutico , Adulto , Ascitis/diagnóstico , Ascitis/cirugía , Femenino , Enfermedades Fetales/tratamiento farmacológico , Hepatitis B Crónica/tratamiento farmacológico , Humanos , Inmunización Pasiva/métodos , Recién Nacido , EmbarazoRESUMEN
Objetivo: Revisar la embriología, los hallazgos clínicos y la apariencia en la tomografía computarizada multidetector (TCMD) del divertículo de Meckel (DM) complicado en adultos. Conclusión: La presencia de un DM en una TCMD debe sospecharse cuando se observa una estructura tubular ciega conectada al íleon terminal por un cuello de tamaño variable. Las reconstrucciones multiplanares y la enterografía por TC pueden ayudar al diagnóstico. En pacientes con síntomas de obstrucción o inflamación intestinal, se debe considerar la posibilidad de un DM complicado; especialmente cuando el íleon terminal y el apéndice vermiforme son normales. La angiografía por TCMD, en casos de hemorragia digestiva aguda, puede identificar extravasación de contraste y acumulación de sangre en el DM. La observación de la arteria onfalomesentérica es diagnóstica de DM. Otros hallazgos en la TCMD, como la presencia de enterolitos o cambios inflamatorios en torno al DM, pueden facilitar el diagnóstico
Objective: To review the embryology, the clinical findings and the appearance in multidetector computed tomography (MDCT) of complicated Meckel's diverticulum (DM) in adults. Conclusion: The presence of an MD in a MDCT should be suspected when a blind tubular structure connected to the terminal ileum by a neck of variable size is observed. Multiplanar 2D reconstructions images and CT enterography can help in the diagnosis. In patients with symptoms of obstruction or intestinal inflammation, the possibility of a complication secondary to a MD should be considered, especially when the terminal ileum and the vermiform appendix are normal. MDCT angiography, in cases of acute gastrointestinal bleeding, can identify contrast extravasation and accumulation of blood in the MD, and could demonstrate the persistent omphalomesenteric artery, which is diagnostic of MD. Other MDCT findings such as the presence of enteroliths or associated inflammatory changes around the MD can facilitate the preoperative diagnosis of MD complications
Asunto(s)
Humanos , Divertículo Ileal/diagnóstico por imagen , Tomografía Computarizada Multidetector/métodos , Divertículo Ileal/cirugía , Obstrucción Intestinal/diagnóstico por imagen , Divertículo Ileal/embriología , Hemorragia Gastrointestinal/diagnóstico por imagenRESUMEN
This study evaluates the role of reverse-transcriptase polymerase chain reaction (RT-PCR) assay for carcinoembryonic antigen (CEA), cytokeratin 19 (CK19), and maspin transcripts to identify breast cancer cells (BCC) in leukapheresis products (LP) collected from breast cancer (BC) patients and compares these results with those obtained using immunocytochemistry (IC). Eighty-four LP obtained from 33 patients with stage II-III BC and control subjects without BC were screened for the presence of BCC by IC and CK19, CEA, and maspin expression using RT-PCR. CEA RT-PCR and IC were the only specific markers, as no false positives were detected in any patients without BC. CK19 RT-PCR gave 11% false positives, whereas maspin RT-PCR with 25% was the most unspecific marker. In LP from BC patients, positive results were observed in 70% and 63% for CK19 and CEA RT-PCR, respectively. For maspin RT-PCR, this percentage was 22%, and for IC it was 17%. There was a good correlation between the CEA and CK19 RT-PCR (p = 0.018). No correlation between CEA and CK19 RT-PCR and IC was found, and although 5 of the 6 IC+ samples were CEA+/CK19+, great discrepancies in the group of IC- samples were observed. Our data suggest that RT-PCR assays for CEA and, to a lesser extent, for CK19 have more sensitivity and specificity than IC to detect BCC in LP.
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Neoplasias de la Mama/patología , Antígeno Carcinoembrionario/análisis , Queratinas/análisis , Leucaféresis , Células Neoplásicas Circulantes , Proteínas/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Serpinas/análisis , Biomarcadores de Tumor/análisis , Neoplasias de la Mama/sangre , Neoplasias de la Mama/química , Neoplasias de la Mama/terapia , Antígeno Carcinoembrionario/genética , Reacciones Falso Positivas , Femenino , Genes Supresores de Tumor , Humanos , Inmunohistoquímica , Queratinas/genética , Proteínas/genética , ARN Mensajero/análisis , Sensibilidad y Especificidad , Serpinas/genética , Estadística como Asunto , Células Tumorales CultivadasRESUMEN
Se han publicado diferentes casos clínicos de pacientes afectos de trombosis con asociación de factores de riesgo, especialmente estados de hipercoagulabilidad. Sin embargo, no hemos encontrado en la literatura un caso como el que presentamos: una mujer de 75 años, evaluada paratrombofilia a raíz de un episodio de trombosis venosa profunda que afectóa su hija, a la que se le ha detectado una acumulación factores de riesgo (13 en total), tanto congénitos como adquiridos, arteriales como venosos que, sorprendentemente, no ha abocado a evento trombótico alguno. Su exposición nos permite incidir sobre los factores de riesgo de la paciente y abrir interrogantes sobre el conocimiento actual de la etiología y etiopatogenia de los fenómenos trombóticos
Some case reports have been published in the literature about thrombosis associated with several risk factors, specially in hypercoagulability state. Nevertheless, we have not found any description of a case like this of hyperthrombophilia status without thrombotic events. We present a 75 years old woman who was assessed for thrombophilia state on occasion of a deep venous thrombosis which affected to her daughter. Many thrombosis risk factors were detected (13 altogether), both inherited and acquired, arterial and venous, that surprisingly, have not led to any thrombosis event. Its description led us to highlight the risk factors of this patient and to open questions about the present knowledge on etiology and etiopathogenesis of thrombotic phenomena
Asunto(s)
Femenino , Anciano , Humanos , Trombofilia/fisiopatología , Trombosis/etiología , Factores de RiesgoRESUMEN
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