Silver Nanoparticles Produced by Laser Ablation and Re-Irradiation Are Effective Preventing Peri-Implantitis Multispecies Biofilm Formation.

Implant-associated infection due to biofilm formation is a growing problem. Given that silver nanoparticles (Ag-NPs) have shown antibacterial effects, our goal is to study their effect against multispecies biofilm involved in the development of peri-implantitis. To this purpose, Ag-NPs were synthesized by laser ablation in de-ionized water using two different lasers, leading to the production of colloidal suspensions. Subsequently, part of each suspension was subjected to irradiation one and three times with the same laser source with which it was obtained. Ag-NPs were immobilized on the surface of titanium discs and the resultant materials were compared with unmodified titanium coupons. Nanoparticles were physico-chemically analysed to determine their shape, crystallinity, chemical composition, and mean diameter. The materials were incubated for 90 min or 48 h, to evaluate bacterial adhesion or biofilm formation respectively with Staphylococcus aureus or oral mixed bacterial flora composed of Streptococcus oralis, Actinomyces naeslundii, Veionella dispar, and Porphyromonas gingivalis. Ag-NPs help prevent the formation of biofilms both by S. aureus and by mixed oral bacterial flora. Nanoparticles re-irradiated three times showed the biggest antimicrobial effects. Modifying dental implants in this way could prevent the development of peri-implantitis.

Laser-Guided Corrosion Control: A New Approach to Tailor the Degradation of Mg-Alloys.

Despite corrosion being commonly seen as a problem to be avoided, applications such as batteries or biodegradable implants do benefit from corrosion-like phenomena. However, current strategies address corrosion control from a global perspective for a whole component, without considering local adaptations to functionality specifications or inhomogeneous environments. Here, a novel concept is presented: the local control and guidance of corrosion through a laser surface treatment. Immersion tests in saline solution of AZ31 magnesium alloy samples show degradation rates reduced up to 15 times with the treatment, owing to a fast passivation after the induced microstructural modifications. By controlling the treatment conditions, the degradation can be restricted to delimited regions and driven towards specific directions. The applicability of the method for the design of tailored degradation biomedical implants is demonstrated and uses for cathodic protection systems and batteries can also be anticipated.

Palladium Nanoparticles Synthesized by Laser Ablation in Liquids for Antimicrobial Applications.

Antibiotic resistance is a leading cause of death worldwide. In this paper, we explore new alternatives in the treatment of infections. Noble metal nanoparticles could help to mitigate this problem. In this work, palladium nanoparticles were synthesized by laser ablation in order to explore their antimicrobial capacity. To obtain palladium nanoparticles, a palladium plate immersed in water, or methanol, was ablated, using two pulsed lasers that emit radiation with wavelengths of 532 nm and 1064 nm, respectively. Pure Pd-NPs with crystalline microstructure and rounded shape were obtained. The nanoparticles' size is more homogeneous if the laser wavelength is 532 nm, and it decreases when methanol is used as solvent, reaching mean diameters smaller than 6 nm. With the objective of studying antimicrobial activity against Staphylococcus aureus, the Pd-NPs were immobilized on the surface of titanium discs. The release of palladium ions was recorded during the first seven days, and the cytotoxicity of the immobilized NPs was also tested with L929 mouse fibroblast cell line. Palladium nanoparticles synthesized by means of the infrared laser in methanol showed a strong inhibitory effect on S. aureus and good cytocompatibility, with no toxic effect on fibroblast cells.

Laser Cutting: A Review on the Influence of Assist Gas.

Assist gas plays a central role in laser fusion cutting. In this work, the aerodynamic interactions between the assist gas and the workpiece are reviewed. An insight into those phenomena that hinder the cutting quality and performance is provided. These phenomena include shock waves, choking, boundary layer separation, etc. The most relevant and promising attempts to overcome these common problems related to the gas dynamics are surveyed. The review of the current scientific literature has revealed some gaps in the current knowledge of the role of the assist gas dynamics in laser cutting. The assist gas interactions have been investigated only under static conditions; and the dynamic interaction with the molten material on the cutting front has not been addressed. New nozzle designs with improved efficiency of molten material removal are required to improve cut quality; and cutting speed in current industrial laser cutting machines; especially in those assisted by new high-brightness laser sources.

Laser Microdrilling of Slate Tiles.

Slate is a natural rock usually used in roofs, façades, and for tiling. In spite of this broad use, the production process of slate tiles requires substantial improvements. An important quantity of slate from the quarry is wasted during the manufacturing of the final product. Furthermore, processes are not automatized and the production lead times can be considerably shortened. Therefore, new processing methods to increase productivity, reduce costs and to provide added value to the final slate product are required. Drilling is an important part of these manufacturing processes. Conventional drilling processes usually cause the breaking of the slate tiles; then, even a higher quantity of material is wasted. To overcome these problems, lasers emerge as a feasible tool to produce holes in this material, since mechanical stresses are not induced on the workpiece. In this work, we have studied the CO2 laser microdrilling of slate tiles. We used a Design of Experiments (DOE) methodology to determine the influence of the laser processing parameters on the hole quality. This work demonstrates the capability of a CO2 laser to produce holes in slate with less than 100 microns in diameter, avoiding any fracture, and with a processing time of less than 50 ms per hole. Finally, this process demonstrates the viability of the production of high-density micron-sized holes in a slate tile for water draining purposes.

Laser irradiation in water for the novel, scalable synthesis of black TiO x photocatalyst for environmental remediation.

Since 1970, TiO2 photocatalysis has been considered a possible alternative for sustainable water treatment. This is due to its material stability, abundance, nontoxicity and high activity. Unfortunately, its wide band gap (≈3.2 eV) in the UV portion of the spectrum makes it inefficient under solar illumination. Recently, so-called "black TiO2" has been proposed as a candidate to overcome this issue. However, typical synthesis routes require high hydrogen pressure and long annealing treatments. In this work, we present an industrially scalable synthesis of TiO2-based material based on laser irradiation. The resulting black TiO x shows a high activity and adsorbs visible radiation, overcoming the main concerns related to the use of TiO2 under solar irradiation. We employed a commercial high repetition rate green laser in order to synthesize a black TiO x layer and we demonstrate the scalability of the present methodology. The photocatalyst is composed of a nanostructured titanate film (TiO x ) synthetized on a titanium foil, directly back-contacted to a layer of Pt nanoparticles (PtNps) deposited on the rear side of the same foil. The result is a monolithic photochemical diode with a stacked, layered structure (TiO x /Ti/PtNps). The resulting high photo-efficiency is ascribed to both the scavenging of electrons by Pt nanoparticles and the presence of trap surface states for holes in an amorphous hydrogenated TiO x layer.

Toward smart implant synthesis: bonding bioceramics of different resorbability to match bone growth rates.

Craniofacial reconstructive surgery requires a bioactive bone implant capable to provide a gradual resorbability and to adjust to the kinetics of new bone formation during healing. Biomaterials made of calcium phosphate or bioactive glasses are currently available, mainly as bone defect fillers, but it is still required a versatile processing technique to fabricate composition-gradient bioceramics for application as controlled resorption implants. Here it is reported the application of rapid prototyping based on laser cladding to produce three-dimensional bioceramic implants comprising of a calcium phosphate inner core, with moderate in vitro degradation at physiological pH, surrounded by a bioactive glass outer layer of higher degradability. Each component of the implant is validated in terms of chemical and physical properties, and absence of toxicity. Pre-osteoblastic cell adhesion and proliferation assays reveal the adherence and growth of new bone cells on the material. This technique affords implants with gradual-resorbability for restoration of low-load-bearing bone.

Production of nanoparticles from natural hydroxylapatite by laser ablation.

Laser ablation of solids in liquids technique has been used to obtain colloidal nanoparticles from biological hydroxylapatite using pulsed as well as a continuous wave (CW) laser. Transmission electron microscopy (TEM) measurements revealed the formation of spherical particles with size distribution ranging from few nanometers to hundred nanometers and irregular submicronic particles. High resolution TEM showed that particles obtained by the use of pulsed laser were crystalline, while those obtained by the use of CW laser were amorphous. The shape and size of particles are consistent with the explosive ejection as formation mechanism.