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1.
J Cardiovasc Pharmacol ; 84(2): 220-226, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-38922584

RESUMEN

ABSTRACT: Sodium-glucose cotransporter-2 (SGLT-2) inhibitors have been shown to reduce the risk of cardiovascular mortality and hospitalizations in patients with heart failure (HF) with preserved or reduced ejection fraction (HFpEF or HFrEF). The mechanism for this benefit is not clear. Endothelial progenitor cells (EPCs) are bone marrow-derived cells able to differentiate into functional endothelial cells and participate in endothelial repair. The aim of this study was to evaluate the effect of SGLT-2 inhibitors on the level and function of EPCs in patients with HF. We enrolled 20 patients with symptomatic HF, 12 with HFrEF and 8 with HFpEF (aged 73.3 ± 10.2 years, 95% men). Blood samples were drawn at 2 time points: baseline and ≥3 months after initiation of SGLT-2 inhibitor therapy. Circulating EPC levels were evaluated by expression of vascular endothelial growth factor receptor-2 (VEGFR-2), CD34, and CD133 by flow cytometry. EPC colony forming units (CFUs) were quantified after 7 days in culture. The proportion of cells that coexpressed VEGFR-2 and CD34 or VEGFR-2 and CD133 was higher following 3 months of SGLT-2 inhibitors [0.26% (interquartile range, IQR 0.10-0.33) versus 0.55% (IQR 0.28-0.91), P = 0.002; 0.12% (IQR 0.07-0.15) versus 0.24% (IQR 0.15-0.39), P = 0.001, respectively]. EPC CFUs were also increased following SGLT-2 inhibitor treatment [23 (IQR 3.7-37.8) versus 79.4 (IQR 25.1-110.25) colonies/10 6 cells, P = 0.0039]. In patients with symptomatic HF, both HFpEF and HFrEF, treatment with SGLT-2 inhibitors is associated with an increase in the level and function of circulating EPCs. This augmentation in EPCs may be a contributing mechanism to the clinical benefit of SGLT-2 inhibitors in patients with HF.


Asunto(s)
Células Progenitoras Endoteliales , Insuficiencia Cardíaca , Inhibidores del Cotransportador de Sodio-Glucosa 2 , Volumen Sistólico , Receptor 2 de Factores de Crecimiento Endotelial Vascular , Humanos , Inhibidores del Cotransportador de Sodio-Glucosa 2/uso terapéutico , Inhibidores del Cotransportador de Sodio-Glucosa 2/farmacología , Masculino , Insuficiencia Cardíaca/fisiopatología , Insuficiencia Cardíaca/tratamiento farmacológico , Insuficiencia Cardíaca/metabolismo , Células Progenitoras Endoteliales/efectos de los fármacos , Células Progenitoras Endoteliales/metabolismo , Células Progenitoras Endoteliales/patología , Anciano , Femenino , Persona de Mediana Edad , Resultado del Tratamiento , Anciano de 80 o más Años , Células Cultivadas , Volumen Sistólico/efectos de los fármacos , Factores de Tiempo , Receptor 2 de Factores de Crecimiento Endotelial Vascular/metabolismo , Biomarcadores/sangre , Antígenos CD34/metabolismo , Antígenos CD34/sangre , Antígeno AC133/metabolismo , Función Ventricular Izquierda/efectos de los fármacos , Transportador 2 de Sodio-Glucosa/metabolismo
2.
Calcif Tissue Int ; 111(3): 300-312, 2022 09.
Artículo en Inglés | MEDLINE | ID: mdl-35505249

RESUMEN

Higher calcium (Ca) absorption would partially compensate for Ca intake below requirements for bone health. Previously, we found that GOS/FOS prebiotic mixture (PM) increases Ca absorption in the colon and retention in bone. Ca absorption and retention are regulated by vitamin D (VD). Hence, it is relevant to explore whether VD insufficiency influences the effect of the PM in the colon. The effect of the PM on Ca, phosphate (IP), and magnesium (Mg) absorption and retention under conditions of VD sufficiency and insufficiency (VDInsuff) was compared using a preclinical model of VDInsuff and low bone mass. Ovariectomized rats were fed isocaloric semisynthetic diets according to AIN-93 M. The diets varied in Ca (0.5% or 0.3%), VD [100 IU% (+ D) or 0 IU% (- D)], and PM (2.5% or 0%) content. The following eight groups were studied: + D0.5; + D0.3; + DPM0.5; + DPM0.3; - D0.5; - D0.3; - DPM0.5; and - DPM0.3. Irrespective of Ca content, VDInsuff did not affect the prebiotic effect of the PM on caecum pH, lactobacillus colony growth, or Mg absorption but significantly decreased its effect on colonic crypt length and cell/crypt and Ca and IP absorption. The PM failed to counterbalance the pro-inflammatory effect of VDInsuff. Moreover, bone retention i.e., bone mineral content and density, bone volume, and bone quality parameters were significantly lower (p < 0.05) and bone turnover significantly was higher (p < 0.05). Although the PM is a useful tool to improve mineral absorption and bone retention, it would seem important to monitor VD nutritional status to ensure the full prebiotic effect in the large intestine.


Asunto(s)
Calcio , Deficiencia de Vitamina D , Animales , Densidad Ósea , Calcio/metabolismo , Calcio de la Dieta/farmacología , Absorción Intestinal , Magnesio/farmacología , Minerales/farmacología , Prebióticos , Ratas , Vitamina D/farmacología , Vitaminas/farmacología
3.
Isr Med Assoc J ; 24(10): 629-633, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-36309856

RESUMEN

BACKGROUND: Multiple myeloma (MM) accounts for approximately 10% of hematological malignancies. The monoclonal immunoglobulin G kappa (IgG-κ) daratumumab can bind to CD38 on MM cells and be detected in serum immunofixation (IF), causing pitfalls in M-protein quantification. OBJECTIVES: To determine the efficacy of mitigating the interference of IgG MM treated with daratumumab. METHODS: Levels of Ig, free light chains (FLC) kappa (κ) and lambda (λ), serum protein electrophoresis (SPE)/IF, and Hydrashift 2/4 assays were assessed following manufacturer's instructions in three patients. RESULTS: Patient 1 was a 70-year-old male diagnosed with IgG-λ MM. The IF distinguished two monoclonal bands (IgG-κ and IgG-λ). With the Hydrashift assay, the daratumumab-anti-daratumumab immune complex shifted the IgG-κ to the α zone, suggesting that the monoclonal IgG-κ band corresponded to daratumumab. Patient 2 was a 63-year-old male with IgG-κ MM who was receiving daratumumab once every other week. SPE/IF assay revealed a faint monoclonal IgG-κ band in the  zone. A stronger monoclonal band was observed after administration. The IgG-κ band disappeared on the Hydrashift assay, while the daratumumab-anti-daratumumab complex appeared as a broad smear in the α-region. Patient 3, a 63-year-old male diagnosed with IgG-λMM, was receiving daratumumab once every other month. The IF assay showed two distinct bands (IgG-κ and IgG-λ) post-daratumumab administration. The shift to the α zone of the IgG-κ bands on the Hydrashift assay confirmed that the additional band observed post-infusion was due to the daratumumab. CONCLUSIONS: The Hydrashift assay can help distinguish daratumumab from endogenous M-spike.


Asunto(s)
Cadenas Ligeras de Inmunoglobulina , Mieloma Múltiple , Masculino , Humanos , Anciano , Persona de Mediana Edad , Inmunoelectroforesis/métodos , Mieloma Múltiple/diagnóstico , Mieloma Múltiple/tratamiento farmacológico , Inmunoglobulina G , Electroforesis
4.
Isr Med Assoc J ; 24(5): 317-319, 2022 May.
Artículo en Inglés | MEDLINE | ID: mdl-35598056

RESUMEN

BACKGROUND: The reduced immune response of maintenance hemodialysis patients to coronavirus disease 2019 (COVID-19) vaccines is a major concern. OBJECTIVES: To analyze the late (6 months after full vaccination) antibody response and compare it to early post-vaccination titer. METHODS: We conducted a multicenter prospective study of 13 hemodialysis units in Israel. RESULTS: We demonstrated that the low titers observed among ESRD patients 2-3 months after vaccination with the Comirnaty vaccine (median 63.8 AU/ml) declined to critically lower values 6 months after full vaccination. (Mediananti S antibodies, 31 AU/ml). Seropositivity significantly declined among hemodialysis patients from 89% to 74% (P < 0.0001), although it did not significantly change among controls. CONCLUSIONS: We recommend all patients on hemodialysis receive a booster COVID-19 vaccine 6 months after the second dose.


Asunto(s)
COVID-19 , Vacunas , Anticuerpos Antivirales , Vacuna BNT162 , COVID-19/prevención & control , Vacunas contra la COVID-19 , Humanos , Estudios Prospectivos , Diálisis Renal , SARS-CoV-2 , Vacunación
5.
J Thromb Thrombolysis ; 52(3): 708-714, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34519015

RESUMEN

Coronavirus disease 2019 (Covid-19) is associated with a high incidence of venous and arterial thromboembolic events. Currently, there are no clinical or laboratory markers that predict thrombotic risk. Circulating immature platelets are hyper-reactive platelets, which are associated with arterial thrombotic events. The aim of this study was to assess whether the proportion of circulating immature platelets is associated with disease severity in Covid-19 patients. Patients admitted with Covid-19 disease were prospectively assessed. Immature platelet count (IPC) and immature platelet fraction (IPF) were measured at admission and at additional time points during the hospital course using the Sysmex XN-3000 auto-analyzer. A total of 136 consecutive patients with Covid-19 were recruited [mean age 60 ± 19 years, 49% woman, 56 (41%) had mild-moderate disease and 80 (59%) had severe disease at presentation]. The median IPF% was higher in patients with severe compared to mild-moderate disease [5.8 (3.9-8.7) vs. 4.2 (2.73-6.45), respectively, p = 0.01]. The maximal IPC value was also higher in patients with severe disease [15 (10.03-21.56), vs 10.9 (IQR 6.79-15.62), respectively, p = 0.001]. Increased IPC was associated with increased length of hospital stay. Patients with severe Covid-19 have higher levels of IPF than patients with mild-moderate disease. IPF may serve as a prognostic marker for disease severity in Covid-19 patients.


Asunto(s)
Plaquetas/virología , COVID-19/virología , SARS-CoV-2/patogenicidad , Trombosis/virología , Adulto , Anciano , COVID-19/sangre , COVID-19/diagnóstico , COVID-19/terapia , Femenino , Mortalidad Hospitalaria , Interacciones Huésped-Patógeno , Humanos , Tiempo de Internación , Masculino , Persona de Mediana Edad , Admisión del Paciente , Recuento de Plaquetas , Valor Predictivo de las Pruebas , Pronóstico , Estudios Prospectivos , Factores de Riesgo , Índice de Severidad de la Enfermedad , Trombosis/sangre , Trombosis/diagnóstico , Factores de Tiempo
6.
J Thromb Thrombolysis ; 51(3): 608-616, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32997333

RESUMEN

Coronavirus disease 2019 (Covid-19) is associated with high incidence of venous and arterial thromboembolic events. Currently, there are no markers to guide antithrombotic therapy in Covid-19. Immature platelets represent a population of hyper-reactive platelets associated with arterial events. This prospective study compared consecutive Covid-19 patients (n = 47, median age = 56 years) to patients with acute myocardial infarction (AMI, n = 100, median age = 59 years) and a group of stable patients with cardiovascular risk factors (n = 64, median age = 68 years). Immature platelet fraction (IPF) and immature platelet count (IPC) were determined by the Sysmex XN-3000 auto-analyzer on admission and at subsequent time-points. IPF% on admission was higher in Covid-19 than the stable group and similar to the AMI group (4.8% [IQR 3.4-6.9], 3.5% [2.7-5.1], 4.55% [3.0-6.75], respectively, p = 0.0053). IPC on admission was also higher in Covid-19 than the stable group and similar to the AMI group (10.8 × 109/L [8.3-18.1], 7.35 × 109/L [5.3-10.5], 10.7 × 109/L [7.7-16.8], respectively, P < 0.0001). The maximal IPF% among the Covid-19 group was higher than the stable group and similar to the AMI group. The maximal IPC in Covid-19 was higher than the maximal IPC in both the stable and AMI groups (COVID-19: 14.4 × 109/L [9.4-20.9], AMI: 10.9 × 109/L [7.6-15.2], P = 0.0035, Stable: 7.55 × 109/L [5.55-10.5], P < 0.0001). Patients with Covid-19 have increased immature platelets indices compared to stable patients with cardiovascular risk factors, and as the disease progresses also compared to AMI patients. The enhanced platelet turnover and reactivity may have a role in the development of thrombotic events in Covid-19 patients.


Asunto(s)
Plaquetas/patología , COVID-19/sangre , Infarto del Miocardio/sangre , Adulto , Anciano , Femenino , Humanos , Pacientes Internos , Masculino , Persona de Mediana Edad , Estudios Prospectivos
7.
Isr Med Assoc J ; 22(8): 494-500, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-33236582

RESUMEN

BACKGROUND: Ferritin, the cellular protein storage for iron, has emerged as a key molecule in the immune system, orchestrating the cellular defense against inflammation. At the end of 2019, the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) rapidly spread throughout China and other countries around the world, resulting in a viral pandemic. OBJECTIVES: To evaluate the correlation between ferritin and disease severity in coronavirus disease-2019 (COVID-19). METHODS: In this cross-sectional study, we obtained clinical and laboratory data regarding 39 hospitalized patients with confirmed COVID-19 from two hospitals in Israel. RESULTS: A significant increase in ferritin levels was demonstrated in patients with moderate and severe disease, compared to patients with mild disease (P = 0.006 and 0.005, respectively). Severe patients had significantly higher levels of ferritin (2817.6 ng/ml) than non-severe patients (708.6 ng/ml) P = 0.02. CONCLUSIONS: In this preliminary cross-sectional study, elevated ferritin levels were shown to correlate with disease severity in 39 patients from Israel with confirmed COVID-19 infection. Our results further strengthen the hypothesis that severe COVID-19 disease might be due to an underlying dysregulated hyperimmune response. In order to identify these patients early and prioritized resources, we believe that all patients with COVID-19 should be screened for hyperferritinemia.


Asunto(s)
COVID-19/sangre , Ferritinas/sangre , Hiperferritinemia/sangre , Gravedad del Paciente , Adulto , Anciano , Biomarcadores/sangre , COVID-19/complicaciones , Estudios Transversales , Femenino , Humanos , Hiperferritinemia/virología , Masculino , Persona de Mediana Edad , SARS-CoV-2 , Adulto Joven
8.
Calcif Tissue Int ; 104(3): 301-312, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30390108

RESUMEN

Menopause is associated with bone loss. Prebiotics increase Ca, inorganic phosphorus (Pi), and Mg absorption, improving bone health. These increases would supply an extra amount of minerals, decreasing bone resorption and possibly reversing ovariectomy-induced bone loss. The present experimental study sought to evaluate the effect of adding a prebiotic GOS/FOS® mixture to a normal or a low Ca diet on Ca, Pi, and Mg absorption, in osteopenic rats. Four groups of n = 8 rats each were OVX, and 8 rats were SHAM operated. All rats were fed a commercial diet for 45 days. They were then fed one of the following diet for 45 days: C-0.5%: SHAM fed AIN 93 M containing 0.5%Ca; O-0.5% and O-0.3%: OVX rats fed AIN 93 M, containing 0.5% or 0.3%Ca, respectively; GF-0.5% and GF-0.3%: OVX rats fed AIN 93 M, containing 0.5% or 0.3%Ca+ 2.5% GOS/FOS®, respectively. At the end of the experimental time point, Ca, P, and MgAbs% was significantly higher in GF-0.5% and GF-0.3% as compared to the remaining groups (p < 0.01). Irrespective of diet Ca content, CTX decreased whereas femur Ca and P content, tibia BV/TV and GPC.Th, lumbar spine and proximal tibia BMD, bone strength, bone stiffness, and elastic modulus increased in the GF-0.5% and GF-0.3% groups as compared to O-0.5% and O-0.3%, respectively (p < 0.05). This prebiotic mixture would be a useful tool to prevent the increase in bone loss associated with menopause and aging.


Asunto(s)
Enfermedades Óseas Metabólicas/metabolismo , Huesos/efectos de los fármacos , Calcio de la Dieta , Dieta , Absorción Intestinal/efectos de los fármacos , Oligosacáridos/farmacología , Animales , Densidad Ósea/efectos de los fármacos , Enfermedades Óseas Metabólicas/etiología , Enfermedades Óseas Metabólicas/patología , Huesos/fisiología , Calcio/deficiencia , Calcio/metabolismo , Calcio/farmacocinética , Calcio de la Dieta/administración & dosificación , Calcio de la Dieta/farmacocinética , Dieta/métodos , Suplementos Dietéticos , Femenino , Fructosa/química , Fructosa/farmacología , Galactosa/química , Galactosa/farmacología , Oligosacáridos/administración & dosificación , Ovariectomía , Ratas , Ratas Wistar
10.
Eur J Nutr ; 55(8): 2445-2458, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26410393

RESUMEN

INTRODUCTION: During growth, protein deprivation impairs epiphyseal growth plate (EGP) height, bone volume (BV) and endochondral ossification. During catch-up growth, Ca availability becomes essential to ensure the extra amount needed to achieve optimal peak bone mass and strength. GOS and FOS improve mineral absorption in the colon. PURPOSE: The effect of a mixture of GOS/FOS® 9:1 added to a 0.5 %Ca (NCa) and a 0.3 %Ca (LCa) diets on Ca, P and Mg absorptions and bone mineralization, density and structure using an experimental model of growing rats recovering from early protein malnutrition was investigated. METHODS: To induce protein malnutrition, rats were fed a low protein diet: 4 % (LPD) during 1 week and then were randomly assigned to recovery groups (R) until day 50 (T = 50) as follows: R0.5 %: NCa; RP0.5 %: NCa + 5.3 % GOS/FOS®; R0.3 %: LCa and RP0.3 %: LCa + 5.3 % GOS/FOS®. Control groups received the 0.5 %Ca or 0.3 %Ca diet from weaning until day 40 or 50. RESULTS: Body weight and length increased in C groups throughout the study; both were arrested in all R during LPD consumption and increased immediately after re-feeding. Independently of dietary Ca content, LS counts, ß-glucosidase and Ca, P and Mg absorption increased, whereas cecum pH, ß-glucuronidase, urease and tryptophanase decreased in RP0.5 %: and RP0.3 %: as compared to the other studied groups (p < 0.01). Prebiotic consumption decreased CTX levels and increased femur Ca, Mg and P contents, total skeleton bone mineral content, proximal tibia and spine BMD, BV, EGP height and hypertrophic zone thickness, stiffness and elastic modulus as compared to recovery groups fed the prebiotic-free diets. CONCLUSION: Under the present experimental conditions, GOS/FOS® mixture induced colonic positive effects, which increased Ca, P and Mg absorption. Thus, consuming the prebiotic-containing diet resulted in an extra amount of minerals that improved bone development in growing rats recovering from protein malnutrition.


Asunto(s)
Calcio de la Dieta/farmacocinética , Oligosacáridos/administración & dosificación , Desnutrición Proteico-Calórica/tratamiento farmacológico , Trisacáridos/administración & dosificación , Animales , Disponibilidad Biológica , Peso Corporal , Densidad Ósea/efectos de los fármacos , Desarrollo Óseo/efectos de los fármacos , Calcificación Fisiológica/efectos de los fármacos , Calcio de la Dieta/administración & dosificación , Calcio de la Dieta/sangre , Ciego/efectos de los fármacos , Ciego/metabolismo , Dieta , Heces/química , Fémur/efectos de los fármacos , Fémur/fisiología , Glucuronidasa/metabolismo , Placa de Crecimiento/efectos de los fármacos , Placa de Crecimiento/fisiología , Absorción Intestinal , Magnesio/administración & dosificación , Magnesio/sangre , Magnesio/farmacocinética , Masculino , Oligosacáridos/sangre , Oligosacáridos/farmacocinética , Fósforo Dietético/administración & dosificación , Fósforo Dietético/sangre , Fósforo Dietético/farmacocinética , Prebióticos/administración & dosificación , Desnutrición Proteico-Calórica/sangre , Ratas , Ratas Wistar , Trisacáridos/sangre , Trisacáridos/farmacocinética , Triptofanasa/metabolismo , Ureasa/metabolismo
11.
Eur J Nutr ; 54(6): 913-23, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25241022

RESUMEN

AIM: Increasing calcium intake is the most effective strategy for avoiding Ca deficit. However, if intake remains inadequate, improving Ca absorption becomes an important tool to optimize Ca homeostasis and bone health. PURPOSE: The effect of a mixture of GOS/FOS(®) 9:1 added to a normal- or low-Ca diets on Ca absorption and bone mineralization, density and structure was investigated, in a model of growing rats. Several colonic parameters to help support the findings were also evaluated. RESULTS: Weanling Wistar rats received one of the four experimental AIN-93G diets: C5: 0.5% Ca; C3: 0.3% Ca; P5: 0.5% Ca + 5.3% GOS/FOS(®); P3: 0.3% Ca + 5.3% GOS/FOS(®) until 50 days (T = 50). At T = 50, lactobacillus and cecum weights were higher, whereas cecum pH was lower in P5 and P3 versus C5 and C3 (p < 0.001). At T = 50, fecal Ca, Mg and P were lower and their absorptions (mg/dL) were higher in P5 and P3 versus C5 and C3, respectively (p < 0.05). Ca, Mg and P absorption % was higher in P5 and P3 versus C5 and C3 (p < 0.001). Femur Ca and P content, bone mineral content, trabecular bone mineral density, tibia length, bone volume, osteoblast surface, stiffness and elastic modulus were higher in P5 and P3 versus C5 and C3 (p < 0.05). Despite the lower Ca content, P3 group reached similar values than C5 in all these latter parameters. CONCLUSIONS: Supplementing diets with the GOS/FOS(®) mixture increased bone mineralization, density and structure due to an increase in Ca, P and Mg absorptions. Thus, this prebiotic mixture may help to improve bone development in a period of high calcium requirements.


Asunto(s)
Huesos/efectos de los fármacos , Calcio de la Dieta/administración & dosificación , Galactosa/administración & dosificación , Oligosacáridos/administración & dosificación , Prebióticos/administración & dosificación , Animales , Fenómenos Biomecánicos , Densidad Ósea/efectos de los fármacos , Desarrollo Óseo/efectos de los fármacos , Huesos/fisiología , Calcificación Fisiológica/efectos de los fármacos , Calcio de la Dieta/farmacocinética , Dieta , Interacciones Farmacológicas , Heces/química , Absorción Intestinal , Masculino , Ratas , Ratas Wistar
12.
Eur J Nutr ; 53(3): 769-78, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24061348

RESUMEN

PURPOSE: A low calcium intake (LCaI) may predispose to obesity, and excessive fat mass may be detrimental to bone. The impact of Ca inadequacy would be greater in subjects predisposed to obesity. LCaI effect on obesity development during the rapid growth period was compared in two strains of rats: spontaneously obese IIMb/ß (O) and Wistar (W). Pregnant rats were fed 0.5% (N) or 0.2% (L) of Ca (OLCa, ONCa, WLCa and WNCa). Male pups were fed the maternal diet until day 60. METHODS: Body composition, lipid profile, glucose homeostasis, 25 hydroxyvitamin D, Ca-phosphorus, and bone metabolism were evaluated. RESULTS: BW and body fat were higher, whereas body protein was lower in OLCa versus ONCa (p < 0.05). OLCa presented the highest body fat, glucose, non-HDL and total cholesterol, TGL, insulin levels, and HOMA-IR, liver weight, and adipose perigonadal plus retroperitoneal pads (p < 0.05). WLCa did not exhibit an increase BW and only showed a slight change in body composition with minor biochemical alterations compared to WNCa (p < 0.05). Osteocalcin, CTX, and proximal tibia and lumbar spine BMDs were lower in O than in W rats fed the same Ca diet (p < 0.05). Body ash and Ca content, and total skeleton BMC/BW were lower in OLCa and WLCa versus their corresponding NCa groups (p < 0.05). CONCLUSION: The negative effect of a low Ca diet on fat mass accumulation and lipid profile may be more evident in rats predisposed to obesity. Nevertheless, low CaI interferes with the normal glucose homeostasis leading to an increase in insulin resistance. Low CaI during early growth may be an obesogenic factor that may persist into adult life and may account for the development of obesity and some of its co-morbidities.


Asunto(s)
Calcio/deficiencia , Dieta/efectos adversos , Obesidad/etiología , Osteoporosis/etiología , Adiposidad , Animales , Densidad Ósea , Remodelación Ósea , Calcio de la Dieta/administración & dosificación , Calcio de la Dieta/uso terapéutico , Femenino , Resistencia a la Insulina , Lactancia , Masculino , Fenómenos Fisiologicos Nutricionales Maternos , Obesidad/metabolismo , Obesidad/patología , Obesidad/prevención & control , Osteoporosis/metabolismo , Osteoporosis/patología , Osteoporosis/prevención & control , Embarazo , Distribución Aleatoria , Ratas Endogámicas , Ratas Wistar , Destete , Aumento de Peso
13.
Eur J Nutr ; 52(1): 297-305, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22457075

RESUMEN

INTRODUCTION: Obese ß rats may be a suitable model to evaluate the association between calcium intake (CaI) and obesity during growth. OBJECTIVE: The present study comparatively evaluated Ca absorption and retention, and changes in body composition in spontaneously genetically obese (ß) male rats fed three different dietary Ca levels: high 0.9% (HCa); normal: 0.5% (NCa); low: 0.2% (LCa). METHODS: Pregnant rats were fed isocaloric diets which varied in Ca content only. Male pups continued feeding the same maternal diet until postnatal day 60. The percentage of Apparent Ca absorption (CaA %), Ca balance (CaB), body composition, glucose, triglycerides (TGL), and insulin levels were evaluated. RESULTS: Food consumption and body weight (BW) were higher in Group LCa than in Groups NCa and HCa (p < 0.01); no differences were observed between the latter two groups. Group LCa presented the highest body fat, liver weight, perigonadal and retroperitoneal fat (p < 0.05); conversely, body ashes and total skeleton bone mineral content were significantly lower compared with animals in both the NCa (p < 0.01) and HCa groups (p < 0.01). CaB (mg/day) reached a plateau at the highest CaI (mg/day) value (r = 0.985, p < 0.001). CaA%, serum glucose, insulin, and TGL levels rose as CaI decreased (p < 0.01). CONCLUSIONS: Although further studies are required, low Ca consumption in this strain of rats could modulate BW inducing changes in several lipid metabolism parameters, which in turn lead to an increase in body fat.


Asunto(s)
Composición Corporal , Calcio de la Dieta/farmacocinética , Calcio/metabolismo , Obesidad/genética , Adiposidad , Animales , Peso Corporal , Dieta , Femenino , Lactancia , Metabolismo de los Lípidos , Masculino , Embarazo , Índice de Embarazo , Ratas , Triglicéridos/sangre
14.
Clin Kidney J ; 15(2): 226-234, 2022 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-35140934

RESUMEN

BACKGROUND: Humoral responses to coronavirus disease 2019 (COVID-19) vaccines in hemodialysis (HD) patients can direct vaccination policy. METHODS: We compared 409 COVID-19-naïve HD patients from 13 HD units in Israel to 148 non-dialysis-dependent COVID-19-naïve controls. Twenty-four previously infected (antinucleocapsid positive) HD patients were analysed separately. Blood samples were obtained ≥14 days post-vaccination (BNT162b2, Pfizer/BioNTech) to assess seroconversion rates and titers of anti-spike (anti-S) and neutralizing antibodies. RESULTS: The median time from vaccination to blood sample collection was 82 days [interquartile range (IAR) 64-87] and 89 days (IQR 68-96) for HD patients and controls, respectively. Seroconversion rates were lower in HD patients compared with controls for both anti-S and neutralizing antibodies (89% and 77% versus 99.3%, respectively; P < 0.0001). Antibody titers were also significantly lower in HD patients compared with controls {median 69.6 [IQR 33.2-120] versus 196.5 [IQR 118.5-246], P < 0.0001; geometric mean titer [GMT] 23.3 [95% confidence interval (CI) 18.7-29.1] versus 222.7 [95% CI 174-284], P < 0.0001, for anti-S and neutralizing antibodies, respectively}. Multivariate analysis demonstrated dialysis dependence to be strongly associated with lower antibody responses and antibody titers waning with time. Age, low serum albumin and low lymphocyte count were also associated with lower seroconversion rates and antibody titers. HD patients previously infected with sudden acute respiratory syndrome coronavirus 2 (SARS-CoV-2) had no difference in their seroconversion rates or antibody titers compared with COVID-19-naïve patients. CONCLUSION: This study demonstrates diminished and waning humoral responses following COVID-19 vaccination in a large and diverse cohort of HD patients, including those previously infected with SARS-CoV-2. Considering these results and reduced vaccine effectiveness against variants of concern, in addition to continued social distancing precautions, a third booster dose should be considered in this population.

15.
Int J Lab Hematol ; 43(5): 966-972, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-33715283

RESUMEN

BACKGROUND: Immature platelets in the circulation can be measured as immature platelet fraction (IPF). Limited data exist regarding IPF during the course of an acute myocardial infarction (AMI), the association between IPF and extent of cardiac damage, and the long-term prognostic implications of IPF in patients with AMI. AIMS: To examine the temporal course of IPF during the first month after AMI, the association between IPF and extent of cardiac damage, and the long-term prognostic effect of IPF in AMI patients. METHODS: Patients with AMI treated with percutaneous coronary intervention (PCI) were examined. IPF was evaluated by a Sysmex XN-3000 autoanalyzer, at 4 time points: baseline; one day post-PCI; 3 days post-PCI, and 30 days post-PCI. The association between peak troponin-T levels and IPF was evaluated. One-year clinical outcomes (cardiac hospitalization, urgent revascularization, or death) were assessed. RESULTS: One hundred patients were included, mean age was 59.5 ± 11.3 years, 82 were men, 27 had diabetes, and 54 were hospitalized with ST-segment elevation myocardial infarction (STEMI) and 46 with non-ST segment elevation myocardial infarction (NSTEMI). The levels of IPF modestly decreased a day after PCI but did not change in subsequent measurements. Peak troponin-T level was significantly associated with the levels of IPF at all 4 time points. IPF levels three days post-PCI were associated with the composite clinical outcome at 1 year. CONCLUSIONS: The levels of IPF following AMI remain relatively stable over a one-month period. Higher levels of IPF during the acute phase of AMI appear to be associated with worse cardiac outcomes at 1 year.


Asunto(s)
Plaquetas/patología , Infarto del Miocardio con Elevación del ST/patología , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Intervención Coronaria Percutánea , Pronóstico , Infarto del Miocardio con Elevación del ST/diagnóstico , Infarto del Miocardio con Elevación del ST/cirugía , Resultado del Tratamiento
16.
In. Ministerio de Salud de Argentina-MSALARG. Comisión Nacional Salud Investiga. Becas de investigación Ramón Carrillo - Arturo Oñativia: anuario 2010. Buenos Aires, Ministerio de Salud, 2012. p.104-105. (127614).
Monografía en Inglés, Español | ARGMSAL | ID: biblio-992219

RESUMEN

INTRODUCCION: La diferencia de calcio y vitamina D es muy habitual a nivel nacional e internacional. Sin embargo, los datos de su relación con la salud bucal y el índice de masa corporal (IMC) son escasos o inexistentes.OBJETIVO: Evaluar el estado nutricional del calcio (Ca) y de la vitamina D, y su relación con el IMC y la salud bucal.METODOS: El estudio se llevó a cabo en alumnos universitarios de la carrera de Nutrición (Universidad Isalud, Buenos Aires), con una población de 65 estudiantes sanos (15 varones y 50 mujeres) de 20 a 30 años. Se realizó una encuesta nutricional, se extrajo sangre en ayunas y la segunda orina de la mañana (basal). Asimismo, se efectuó un examen bucodental y una densitometría ósea de esqueleto total por DXA (Lunar).RESULTADOS: El IMC promedio fue de 20,7 ± 4,2 para mujeres y 23,5 ± 2,8 para hombres. El 100% del grupo presentó una densidad mineral ósea normal, con un T-Score > -2,5. Sólo el 19% y el 15% de mujeres y hombres, respectivamente, mostraron ingestas de calcio (ICa) igual o superior a las recomendadas. El 34% de mujeres y el 20% de hombres presentaron niveles de 25 hidroxivitamina D (25 OHD) considerados adecuados (> 30 ng/mL). El diagnóstico clínico bucodental mostró que el 100% de los estudiantes presentaba gingivitis marginal crónica asociada a placa. Un 61% de mujeres y un 82% de hombres tenían momentos de azúcar > 4, lo cual se encuentra asociado al aumento en el riesgo de caries. También se observó una pérdida parcial de piezas dentarias en el 29% de mujeres y el 45% de hombres.CONCLUSIONES: Existe una alta deficiencia de vitamina D y un bajo consumo de Ca, que pueden afectar enormemente el futuro estado del esqueleto y la salud bucodental. Una adecuada educación nutricional ayudaría a mejorar el estado nutricional de los individuos y su calidad de vida, hecho que en definitiva redundaría en beneficios en los costos de Salud Pública.


INTRODUCTION: Calcium (Ca) and Vitamin D deficiencies are widespread throughout the world. However, studies about their relationship with oral health and body mass index (BMI) are scarce or do not exist.OBJECTIVE: Assess nutritional status of Ca and vitamin D and their relationship with oral health and BMI.METHODS: The study was performed with 65 healthy students from the School of Nutrition at Isalud University, Buenos Aires (15 men and 50 women, 20- to 30-year-old). Weekly consumption-frequency and dietary practices were recorded. Blood and 2-hour-urine samples were obtained in a fasting state. Dental-oral status and total skeletal bone mineral densitometry (BMD) by DXA were assessed.RESULTS: The body mass index (BMI) was 20.7 ± 4.2 for women and 23.5 ± 2.8 for men. 100% of the students presented a normal bone mineral density with a T-score > 2.5. Only 19% of woman and 15% of men covered the recommended Ca intake. 34% of women and 20% of men presented vitamind D recommended nutritional values (>30 ng/mL). Oral diagnosis showed 100% of the students had chronic gingivitis; 61% and 82% of women and men respectively had high consumption of sugar > 4 with a high caries probability association. 29% of women and 45% of men had a partial tooth loss.CONCLUSIONS: There is a high vitamin D and Ca intake deficiency that could affect the skeleton status and oral health in the future. Adequate nutrition education would help to improve the nutritional status of individuals and their quality of life, a fact that ultimately result in cost benefits of Public Health.


Asunto(s)
Adulto , Calcio , Calidad de Vida , Desarrollo Óseo , Salud Bucal , Vitamina D , Índice de Masa Corporal , Argentina , Salud Pública
17.
In. Ministerio de Salud de Argentina-MSALARG. Comisión Nacional Salud Investiga. Becas de investigación Ramón Carrillo - Arturo Oñativia: anuario 2010. Buenos Aires, Ministerio de Salud, 2012. p.104-105. (127572).
Monografía en Inglés, Español | BINACIS | ID: bin-127572

RESUMEN

INTRODUCCION: La diferencia de calcio y vitamina D es muy habitual a nivel nacional e internacional. Sin embargo, los datos de su relación con la salud bucal y el índice de masa corporal (IMC) son escasos o inexistentes.OBJETIVO: Evaluar el estado nutricional del calcio (Ca) y de la vitamina D, y su relación con el IMC y la salud bucal.METODOS: El estudio se llevó a cabo en alumnos universitarios de la carrera de Nutrición (Universidad Isalud, Buenos Aires), con una población de 65 estudiantes sanos (15 varones y 50 mujeres) de 20 a 30 años. Se realizó una encuesta nutricional, se extrajo sangre en ayunas y la segunda orina de la mañana (basal). Asimismo, se efectuó un examen bucodental y una densitometría ósea de esqueleto total por DXA (Lunar).RESULTADOS: El IMC promedio fue de 20,7 ± 4,2 para mujeres y 23,5 ± 2,8 para hombres. El 100% del grupo presentó una densidad mineral ósea normal, con un T-Score > -2,5. Sólo el 19% y el 15% de mujeres y hombres, respectivamente, mostraron ingestas de calcio (ICa) igual o superior a las recomendadas. El 34% de mujeres y el 20% de hombres presentaron niveles de 25 hidroxivitamina D (25 OHD) considerados adecuados (> 30 ng/mL). El diagnóstico clínico bucodental mostró que el 100% de los estudiantes presentaba gingivitis marginal crónica asociada a placa. Un 61% de mujeres y un 82% de hombres tenían momentos de azúcar > 4, lo cual se encuentra asociado al aumento en el riesgo de caries. También se observó una pérdida parcial de piezas dentarias en el 29% de mujeres y el 45% de hombres.CONCLUSIONES: Existe una alta deficiencia de vitamina D y un bajo consumo de Ca, que pueden afectar enormemente el futuro estado del esqueleto y la salud bucodental. Una adecuada educación nutricional ayudaría a mejorar el estado nutricional de los individuos y su calidad de vida, hecho que en definitiva redundaría en beneficios en los costos de Salud Pública.


INTRODUCTION: Calcium (Ca) and Vitamin D deficiencies are widespread throughout the world. However, studies about their relationship with oral health and body mass index (BMI) are scarce or do not exist.OBJECTIVE: Assess nutritional status of Ca and vitamin D and their relationship with oral health and BMI.METHODS: The study was performed with 65 healthy students from the School of Nutrition at Isalud University, Buenos Aires (15 men and 50 women, 20- to 30-year-old). Weekly consumption-frequency and dietary practices were recorded. Blood and 2-hour-urine samples were obtained in a fasting state. Dental-oral status and total skeletal bone mineral densitometry (BMD) by DXA were assessed.RESULTS: The body mass index (BMI) was 20.7 ± 4.2 for women and 23.5 ± 2.8 for men. 100% of the students presented a normal bone mineral density with a T-score > 2.5. Only 19% of woman and 15% of men covered the recommended Ca intake. 34% of women and 20% of men presented vitamind D recommended nutritional values (>30 ng/mL). Oral diagnosis showed 100% of the students had chronic gingivitis; 61% and 82% of women and men respectively had high consumption of sugar > 4 with a high caries probability association. 29% of women and 45% of men had a partial tooth loss.CONCLUSIONS: There is a high vitamin D and Ca intake deficiency that could affect the skeleton status and oral health in the future. Adequate nutrition education would help to improve the nutritional status of individuals and their quality of life, a fact that ultimately result in cost benefits of Public Health.


Asunto(s)
Adulto , Calcio , Vitamina D , Salud Bucal , Índice de Masa Corporal , Desarrollo Óseo , Calidad de Vida , Argentina , Salud Pública
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