Thomas Willis, a pioneer in translational research in anatomy (on the 350th anniversary of Cerebri anatome).

The year 2014 marked the 350th anniversary of the publication in London of Cerebri anatome, a ground-breaking work of neuroscience heavily influenced by the political and cultural context of Baroque Europe and mid-17th century England. This article aims to review the work of the English physician and anatomist Thomas Willis, specifically with regard to the contents of his Cerebri anatome. Willis's academic and professional career was influenced by the turbulent period of the English Civil War during which he studied medicine. Willis went from chemistry to dissection arguably because of his need to justify the body-brain-soul relationship. As a result, he became a fellow of a select club of eminent experimentalists, and afterward was a Fellow of the Royal Society. Later on, he went to London, leaving the academic life to dedicate himself fully to the profession of medicine. As a physician, Willis did not base his practice on aphorisms but on a 'bench to bedside' approach to medicine, while studying neuroanatomy--covering embryology, comparative anatomy and pathological anatomy--as a basis for the comprehension of neurological pathology. He developed innovative anatomical methods for the preservation and dissection of the brain, injection of coloured substances and illustration of his findings. In Cerebri anatome, Willis recognized the cerebral cortex as the substrate of cognition. He also claimed that the painful stimuli came from the meninges, but not from the brain itself. He explained for the first time the pathological and functional meaning of the brain's circular arterial anastomosis, which is named after him. He also specified some features of the cranial origin of the sympathetic nerves and coined the term 'neurologie'. Cerebri anatome marked the transition between the mediaeval and modern notions of brain function, and thus it is considered a cornerstone of clinical and comparative anatomy of the nervous system. The new contributions and methods employed by Willis justify his place as a father of neurology and a pioneer of translational research.

Latin American Anatomists' views on human body dissection and donation.

BACKGROUND: Studies abound regarding the medical students' views on the importance of anatomy and the dissection of human bodies, but little is known about the views of Latin American Anatomists. METHODS: A survey was carried out to test several hypotheses among anatomists of the Americas about how they perceive their professional identity, the use and role of dissection in their undergraduate courses, and the approval degree of bequeathing their body for anatomical teaching/research; another goal was ascertaining to what extent their attitude on these topics depended on gender, length of teaching experience and belief in the afterlife. RESULTS: One hundred and forty-five anatomists from thirteen Latin American and Caribbean Countries took the survey; 79% stated the main role of an anatomist is teaching; 34% recorded their undergraduate students dissected human cadavers as part of their anatomy lab course-undergraduates dissecting less in the less experienced anatomists' courses (p = 0.0002). Most anatomists said dissection was a training tool for undergraduate students, a tool for developing professional skills, and a tool to help control emotions-most experienced anatomists stood out from the rest saying dissection is only to teach anatomy (p < 0.001), even if such response was the least valued by them among all replies. Men differed from women in valuing dissection as a tool to help control emotions (p = 0.006); less experienced anatomists held the opposite (p < 0.0001). Approval of a close doctor-patient relationship diverged, being different between the most and the least experienced anatomists (p = 0.01). Anatomists said they would donate only their organs (44%), whole body (9%) and both organs and body (46%). Undecided anatomists about the belief in life after death were the least in favor of donation (p = 0.05).

Toledo School of Translators and their influence on anatomical terminology.

Translation facilitates transmission of knowledge between cultures. The fundamental transfer of anatomic terminology from the Ancient Greek and Islamic Golden Age cultures, to medieval Latin Christendom took place in the so-called Toledo School of Translators in the 12th-13th centuries. Translations made in Toledo circulated widely across Europe. They were the foundation of scientific thinking that was born in the boards of first universities. In Toledo, Gerard of Cremona translated Avicenna's Canon of Medicine, the key work of Islamic Golden Age of medicine. Albertus Magnus, Mondino de Luzzi and Guy de Chauliac, the leading authors of anatomical Latin words in the Middle Ages, founded their books on Gerard's translations. The anatomical terms of the Canon retain auctoritas up to the Renaissance. Thus, terms coined by Gerard such as diaphragm, orbit, pupil or sagittal remain relevant in the current official anatomical terminology. The aim of the present paper is to bring new attention to the highly significant influence that the Toledo School of Translators had in anatomical terminology. For this, we shall review here the onomastic origins of a number of anatomical terms (additamentum; coracoid process; coxal; false ribs; femur; panniculus; spondylus; squamous sutures; thorax; xiphoid process, etc.) which are still used today.

The role of an expert discussion panel on shaping the views of the undergraduate in health sciences on the use of stem cells and pre-implantation embryos / La influencia de un panel de discusión de expertos en la formación de opinión sobre el uso de células madre y embriones preimplantacionales en los estudiantes de ciencias de la salud de pregrado

INTRODUCTION: There are four objectives to this paper: (1) To determine whether undergraduates enrolled in Health-Sciences studies agree with the use of human stem cells for medical research, treatment and genetic uses. (2) Whether they would consider the use of pre-implantation-embryos for medical research. (3) Whether attitudes toward the previous two issues are linked to gender, field of study, transcendental/spiritual convictions and political biases. (4) A panel of discussion will modify their opinion. RESULTS: The present study shows that, before attending a discussion panel session, media was the main source of information that the students had on the surveyed topics. A discussion panel was useful for clarifying respondents' opinions on the explored items. Significantly, the discussion panel had an influence on those respondents who did not have a formed opinion on the explored items. CONCLUSIONS: A discussion panel is a convenient, but limited tool, in the shaping of undergraduate opinions on ethically controversial scientific matters

INTRODUCCIÓN: Los objetivos de este artículo son conocer si: 1) los estudiantes de pregrado matriculados en titulaciones de grado de ciencias de la salud están de acuerdo con la utilización de las células madre humanas para la investigación médica de los embriones preimplantatorios, la cura de enfermedades y los usos génicos; 2) consideran el uso de los embriones preimplantatorios humanos para la investigación; 3) las actitudes hacia los 2 temas anteriores están relacionadas con el género, el grado universitario en curso, la afiliación política y las convicciones trascendentales o espirituales, y 4) conocer si un panel de discusión, con expertos, modifica esas opiniones. RESULTADOS: Los resultados del presente estudio mostraron que antes del panel de discusión, los medios de comunicación eran la principal fuente de información de los encuestados sobre los temas estudiados. El panel de discusión fue útil para aclarar las opiniones de los encuestados, aprobar o desaprobar los ítems explorados. Significativamente, el panel de discusión influyó en los encuestados que dijeron que no tenían una opinión formada sobre los ítems explorados antes del panel de discusión. CONCLUSIONES: El panel de discusión es una herramienta conveniente pero limitada en la formación de las opiniones de los estudiantes de pregrado en titulaciones de ciencias de la salud sobre cuestiones científicas éticamente controvertidas

Widespread Horizontal Connections Arising from Layer 5/6 Border Inverted Cells in Rabbit Visual Cortex.

Herein we describe the inverted cells [defined as those projection neurons having a major dendritic shaft abpially oriented (Bueno-López et al., Eur. J. Neurosci., 3, 415, 1991)] originating a unique set of cortical connections characterized by extraordinarily widespread horizontal distribution. Single and multiple injections of wheatgerm agglutinin - horseradish peroxidase were made in areas 17 and 18 and the resulting retrograde labelling in the cortex was analysed. The findings were assessed in independent control experiments in which Fluoro-Gold was used as retrograde tracer. Following single injections in area 17 several separate patches of labelled cells comprising layers 2 - 6 were consistently found in area 18. In addition to these associational cells a number of labelled cells appeared at the layer 5/6 border but were distributed over most of the tangential extent of the visual occipital cortex. This widespread pattern was particularly striking in brains after multiple injections. In these brains a conspicuous band of labelled cells at the 5/6 border radiated from the injection sites, making up an apparently continuous horizontal sheet that intersected the striate - extrastriate boundary and merged with the patches of labelled cells in area 18 and beyond. Most of the cells in the 5/6 border band were inverted cells (82%; n=2081). Injections in area 18 failed to produce such a widespread set of labelled cells in area 17. The functional significance of these connections furnished by the 5/6 border inverted cells remains to be determined, but their distribution would allow for convergent/divergent binding interactions both intra-areally (within area 17) and inter-areally (from area 18 to area 17).

Targets and Laminar Distribution of Projection Neurons with 'Inverted' Morphology in Rabbit Cortex.

This study examines the axonal projections of so-called inverted pyramids and other neurons with their major dendritic shaft oriented in the direction of the white matter ('inverted cells') in the adult rabbit cortex. Single injections of horseradish peroxidase wheat germ agglutinin were made into cortical or subcortical sites. The resulting retrograde labelling in the cortex was analysed and the distribution across areas and layers of inverted cells contributing to each of these projections was estimated. In addition, the radial distribution of inverted cells was independently determined from rapid Golgi-impregnated and Nissl-stained material. All three procedures revealed that inverted cells lay overwhelmingly in infragranular layers, but congregated at the border between layers 5 and 6. Inverted cells, identified by retrograde labelling, seldom furnished non-telencephalic centres; in contrast, these cells constituted a major source for the projections to the ipsi- or the contralateral cortex, the claustrum or the nucleus caudatus. In general, each set of inverted cells (when defined by its specific destination as a group) was located below the typically oriented cells whose axons were aimed at the same target. Thus, the inverted cells of the rabbit cortex are characterized not only by their unique morphology and their corticocortical, corticoclaustral and corticostriatal projections, but also by their distinctive radial locations. These findings suggest that inverted cells, even though possibly composed of different cell types, are a specific class of projection neurons.

Practical teaching of preclinical anatomy

We illustrate here why today practical teaching in preclinical anatomy is important and why the use of human cadavers is still essential for learning human anatomy by taking two examples. We explain why it is important for a student to be able to dissect and learn anatomy by exploratory anatomy. Several alternatives are discussed and modern teaching tools are illustrated with on-line and computer-based resources, anatomical models, reconstructions and radiographic images that could supplement the traditional dissection courses. Newer techniques such as anatomical body painting, projections, ultrasound or living anatomy may help in the understanding of topographical anatomy. We underline the authenticity that comes from using human tissue and consider the strengths and limitations of different teaching tools. Here we discuss also how far one should go in teaching anatomical variations in preclinical teaching. In Europe there is no consensus regarding anatomical teaching, and each institution has its own curriculum. It would be helpful to set up an anatomical data bank with images and PowerPoint slides that could be used in teaching programs. Here the Trans-European Pedagogic Anatomical Research Group (TEPARG) for Europe and the International Federation of Associations of Anatomists (IFAA) at an international level could play an essential role

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Anatomy, respect for the body and body donation - a guide for good practice

As pointed out by many anatomists worldwide, an international debate on body donation and the use of the dissection of human cadavers in health-care studies is needed. Numerous countries have no donation programs in place, or difficulties in obtaining bodies for anatomy teaching. Consequently, the International Federation of Associations of Anatomists (IFAA), in their 2014 Beijing Meeting, has proposed the creation of an international body donation network and the provision of international help to countries with difficulties setting up donation programs. In two previous publications by the Trans-European Pedagogic Anatomical Research Group (TEPARG), a number of legal, ethical, cultural and religious aspects of current practice in body donation in European countries were reviewed. Now, we bring here to international attention some other aspects of body donation in Switzerland and Spain. In doing so, we wish to address specific questions, e.g., who can donate, where to enroll in the donation program, best practice in interacting with relatives of the deceased donors, issues raised by commercialization in the use of some human bodies, use of long-term preserved body parts and body parts nearly permanently preserved by means of plastination, ethical use of human bodies for medical research and public display. We also propose some principles of good practice for body donation

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The underside of the cerebral cortex: layer V/VI spiny inverted neurons.

This paper presents an account of past and current research on spiny inverted neurons--alternatively also known as 'inverted pyramidal neurons'--in rats, rabbits and cats. In our laboratory, we have studied these cells with a battery of techniques suited for light and electron microscopy, including Nissl staining, Golgi impregnation, dye intracellular filling and axon retrograde track-tracing. Our results show that spiny inverted neurons make up less than 8.5 and 5.5% of all cortical neurons in the primary and secondary rabbit visual cortex, respectively. Infragranular spiny inverted neurons constitute 15 and 8.5% of infragranular neurons in the same animal and areas. Spiny inverted neurons congregate at layers V-VI in all studied species. Studies have also revealed that spiny inverted neurons are excitatory neurons which furnish axons for various cortico-cortical, cortico-claustral and cortico-striatal projections, but not for non-telencephalic centres such as the lateral and medial geniculate nuclei, the colliculi or the pons. As a group, each subset of inverted cells contributing to a given projection is located below the pyramidal neurons whose axons furnish the same centre. Spiny inverted neurons are particularly conspicuous as a source of the backward cortico-cortical projection to primary visual cortex and from this to the claustrum. Indeed, they constitute up to 82% of the infragranular cells that furnish these projections. Spiny inverted neurons may be classified into three subtypes according to the point of origin of the axon on the cell: the somatic basal pole which faces the cortical outer surface, the somatic flank and the reverse apical dendrite. As seen with electron microscopy, the axon initial segments of these subtypes are distinct from one another, not only in length and thickness, but also in the number of received synaptic boutons. All of these anatomical features together may support a synaptic-input integration which is peculiar to spiny inverted neurons. In this way, two differently qualified streams of axonal output may coexist in a projection which arises from a particular infragranular point within a given cortical area; one stream would be furnished by the typical pyramidal neurons, whereas spiny inverted neurons would constitute the other source of distinct information flow.

The legal and ethical framework governing Body Donation in Europe - 1st update on current practice

Previously, we have reported on the legal and ethical aspects and current practice of body donation in several European countries, reflecting cultural and religious variations as well as different legal and constitutional frameworks. We have also established good practice in body donation. Here we shall further extend the legal and ethical frameworks in place and also focus on novelties in the law and different directives. Of particular interest are points that address the commercialization of human bodies and body parts and weaknesses in the legal directives. Therefore, it is important to define what is ethical and what needs to be considered unethical in body donation and the subsequent utilisation of human bodies for teaching and research (AU)

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