Improvement of Magnetic Saturation in Fe3O4@Y2O3:Eu3+ Nanocomposites Through the Manipulation of Eu3+ Activators.

Fe3O4@Y2O3:Eu3+ nanocomposites and Y2O3:Eu3+ nanophosphors were synthesized using the hydrothermal method. Nanocomposites were analyzed using X-ray diffraction (XRD), Rietveld refinements, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, photoluminescence (PL), vibrating sample magnetometer (VSM), and high-resolution transmission electron microscopy (HRTEM). Nanocomposites exhibit superparamagnetic behavior that improves with Eu3+, resulting in increased magnetic saturation. In contrast to Y2O3:Eu3+ nanophosphors, the Fe3O4@Y2O3:Eu3+ nanocomposites display a distinctive characteristic whereby the photoluminescence intensity increases with a reduced concentration of Eu3+. The requirement of increasing the thickness of the Y2O3:Eu3+ outer layer to achieve improved light emission can be circumvented by solely manipulating the concentration of activators, without compromising the magnetic saturation of the nanocomposites. The luminescent and magnetic characteristics of Fe3O4@Y2O3:Eu3+ nanocomposites can be readily optimized using straightforward synthesis parameters, making them promising candidates for potential applications in theranostic medicine.

Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases in Spain: microbiological and clinical features.

Extended-spectrum ß-lactamases (ESBL) of the CTX-M, SHV, and TEM families were recognized in 76 (67%), 31 (27%), and 6 (5%) isolates, respectively, among 162 ESBL-producing Klebsiella pneumoniae (ESBL-Kp) strains obtained in a multicenter study in Spain. Predisposing factors for ESBL-Kp acquisition included invasive procedures, mechanical ventilation, and previous antimicrobial use.

Folate- and glucuronate-functionalization of layered double hydroxides containing dysprosium and gadolinium and the effect on oxidative stress in rat liver mitochondria.

Zinc/aluminum layered double hydroxide (LDH) particles were prepared by alkaline precipitation in the presence of dysprosium and dysprosium/gadolinium cations. The particles formed were stable against exchange reactions with folate or glucuronate ions since these organic ions exclusively functionalized the external surface of the layered double hydroxides. While the dysprosium derivatives reached magnetization susceptibilities between 2.06 × 10-5 and 2.20 × 10-5 cm3/g, the samples simultaneously containing dysprosium and gadolinium decreased to a range between 1.08 × 10-5 and 1.73 × 10-5 cm3/g. This last sample was tested as a magnetic resonance imaging contrast agent and demonstrated a reduction in T1 and T2 relaxation times in a linear dependence with the LDH concentration. The oxidative stress assays in rat liver mitochondria demonstrated the low toxicity of the composition simultaneously containing dysprosium and gadolinium as well as the functionalization product with glucuronate ions, suggesting the potential of these particles to design alternative MRI contrast agents.

Potential trade-offs between eliminating plastics and mitigating climate change: An LCA perspective on Polyethylene Terephthalate (PET) bottles in Cornwall.

The aim of this study is to investigate whether eliminating plastics entirely under existing waste infrastructure and management practices could have an adverse effect on climate change, using a case study on the hypothetical substitution of Polyethylene Terephthalate (PET) with glass as the material for bottling liquids in the domestic sector in Cornwall, England. A life cycle environmental impacts-based model was created using high resolution local data on household waste and current management practices in combination with Life Cycle Assessment (LCA) datasets. The model allows users to define key system parameters such as masses of materials, transport options and end-of-life processes and produces results for 11 environmental impact categories including the Global Warming Potential (GWP). The results from the application of this model on the case study of Cornwall have shown that the substitution of PET with glass as the material for bottling under the current waste infrastructure and management practices could lead to significant increases in GWP and hinder efforts to tackle climate change. A sensitivity analysis of the glass/PET mass ratio suggests that in order to achieve equal GWP the glass bottles need to become approximately 38% of the weight they are now. Increasing the recycled content and decreasing losses during the recycling processes could also help lower the GWP by 18.9% and 14.5%, respectively. This model can be expanded further to include more types of plastics and other regions to evaluate designs of new regional circular economy with less plastics waste and pollution. Our study suggests that it is necessary and crucial to consider the specific waste infrastructure and management practices in place and use science-based models that incorporate life cycle thinking to evaluate any solutions to plastics pollution in order to avoid problem shifting.

Detection of plasmid-mediated quinolone resistance genes in clinical isolates of Enterobacter spp. in Spain.

We have studied by PCR and DNA sequencing the presence of the qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA, intI1, and ISCR1 genes in 200 clinical isolates of Enterobacter cloacae (n = 153) and E. aerogenes (n = 47) consecutively collected between January 2004 and October 2005 in two hospitals located in Santander (northern Spain) and Seville (southern Spain). Mutations in the quinolone resistance-determining region of gyrA and parC also were investigated in organisms containing plasmid-mediated quinolone resistance genes. The isolates had different resistant phenotypes, including AmpC hyperproduction, extended-spectrum beta-lactamase production, resistance or decreased susceptibility to quinolones, and/or resistance to aminoglycosides. Among the 116 E. cloacae isolates from Santander, qnrS1, qnrB5, qnrB2, and aac(6')-Ib-cr were detected in 22 (19%), 1 (0.9%), 1 (0.9%), and 3 (2.6%) isolates, respectively. Twenty-one, 17, and 2 qnrS1-positive isolates also contained bla(LAP-1), intI1, and ISCR1, respectively. A qnrB7-like gene was detected in one E. aerogenes isolate from Santander. No plasmid-mediated quinolone resistance gene was detected in the isolates from Seville. The qnrS1-containing isolates corresponded to four pulsed-field gel electrophoresis patterns and showed various levels of resistance to quinolones. Six isolates were susceptible to nalidixic acid and presented reduced susceptibility to ciprofloxacin. The qnrS1 gene was contained in a conjugative plasmid of ca. 110 kb, and when the plasmid was transferred to recipient strains that did not have a specific mechanism of quinolone resistance, the ciprofloxacin MICs ranged from 0.047 to 0.125 microg/ml.

Magnetic properties of synthetic eumelanin--preliminary results.

We report an experimental and theoretical study of magnetic properties of synthetic eumelanin. The magnetization curves are determined by using both a vibrating sample magnetometer and a superconducting quantum interferometer device in an extended range of magnetic fields ranging from -10 kOe to 10 kOe at different temperatures. We find that the eumelanin magnetization can be qualitatively explained in terms of a simple model of dipolar spheres with an intrinsic magnetic moment. The latter one is experimentally measured by using X-band electron paramagnetic resonance. Our findings indicate that synthetic melanins are superparamagnetic.

Characterisation of integrons containing the plasmid-mediated quinolone resistance gene qnrA1 in Klebsiella pneumoniae.

The aim of this study was to determine the structural relationships of qnrA1 and other resistance genes in four integrons contained in four clinical isolates of Klebsiella pneumoniae. In the four integrons, the sequences surrounding qnrA1 were similar to those described for pMG252 (accession no. AY070235). The four integrons carried a class 1 integrase gene belonging to the complex class 1 integron. Three of the strains contained an identical integron coding for resistance to beta-lactams, aminoglycosides, chloramphenicol and trimethoprim. The fourth strain contained a different integron coding for resistance to beta-lactams, aminoglycosides and chloramphenicol. Downstream of the last integron, copies of IS6100 and IS26 were present. We describe two new and different integrons containing qnrA1. These integrons code for resistance to different groups of antimicrobial agents from K. pneumoniae clinical strains isolated in the USA.

Evaluation of the carbapenem inactivation method (CIM) for detecting carbapenemase activity in enterobacteria.

OBJECTIVES: The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. METHODS: A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation. A clear inhibition zone (≥20 mm) was considered indicative of no-carbapenemase activity. RESULTS: All KPC, NDM, VIM, IMP or OXA-48 producing strains were unequivocally detected with the CIM test. CIM false negative results were obtained with eleven Enterobacter cloacae producing GES-6. Two other E. cloacae not producing carbapenemase (one with SHV-12, one hyperproducing AmpC) were positive by the test. The sensitivity and specificity of the assay compared to those of molecular methods were 85.7% and 95.7%, respectively. CONCLUSIONS: The CIM method proved to be inexpensive and easy to interpret. It provided less than optimal results in the detection of GES-6 activity.

A high-resolution frequency variable experimental setup for studying ferrofluids used in magnetic hyperthermia.

A scanning system for specific absorption rate of ferrofluids with superparamagnetic nanoparticles is presented in this study. The system contains an induction heating device designed and built with a resonant inverter in order to generate magnetic field amplitudes up to 38 mT, over the frequency band 180-525 kHz. Its resonant circuit involves a variable capacitor with 1 nF of capacitance steps to easily select the desired frequency, reaching from 0.3 kHz/nF up to 5 kHz/nF of resolution. The device performance is characterized in order to compare with the theoretical predictions of frequency and amplitude, showing a good agreement with the resonant inverters theory. Additionally, the setup is tested using a synthetic iron oxide with 10 ± 1 nm diameter suspended in liquid glycerol, with concentrations at 1%. Meanwhile, the temperature rise is measured to determine the specific absorption rate and calculate the dissipated power density for each f. This device is a suitable alternative to studying ferrofluids and analyzes the dependence of the power absorption density with the magnetic field intensity and frequency.

Evaluation of antimicrobial susceptibility of bacteria containing the qnr gene and FOX-5 beta-lactamase by four automated systems.

The accuracy and performance of four automated instruments (BD Phoenix, MicroScan WalkAway, VITEK-2 and Wider) were evaluated for susceptibility testing of fluoroquinolones and beta-lactams with four clinical isolates of Klebsiella pneumoniae and the corresponding Escherichia coli transconjugants containing a plasmid carrying the qnr gene and coding for FOX-5 production. No major or very major errors were detected with the MicroScan system. Many of the minor errors for both quinolones and beta-lactams clustered around the intermediate breakpoints.

[Paradoxical reaction to midazolam after its use as a sedative in regional anesthesia]. / Reacción paradójica al midazolam tras su uso como sedante en anestesia regional.

We report 3 cases of paradoxical reaction to midazolam after being used for sedation during regional anesthesia. The picture was characterized by a marked aggressiveness. In one case the reaction was treated with general anesthesia, whereas in the other two patients the clinical picture was rapidly reversed by administration of flumazenil. We describe the possible causative mechanisms of this reaction as well as their treatment.

[Acute correction of nocturnal hypoxemia and sleep pattern using continuous nasal positive pressure in patients with obstructive sleep apnea syndrome]. / Corrección aguda de la hipoxemia nocturna y del patrón del sueño mediante la aplicación continua de presión positiva nasal en enfermos con el síndrome de la apnea obstructiva del sueño.

In order to find out the effectiveness of the nasal CPAP in the treatment of obstructive sleep apnea (OSA), we studied 30 patients with this diagnosis corroborated by nocturnal polysomnography. The average age was 46.37 +/- 11.58 years, 97% were males and 90% were overweight, with a weight of 101.24 +/- 15.57 Kg. All the patients were evaluated before and after the application of nasal CPAP with a pressure of 12.9 +/- 4.35 cm of H2O with a FIO2 of 21%. The sleep efficiency index improved from 0.62 +/- 0.03 to 0.84 +/- 0.02 (p < 0.0001). The maximum duration of the apneas diminished from 65.5 +/- 6.5 seconds to 19 +/- 3.7 seconds (p < 0.0001), and the minimum level of O2 saturation of the hemoglobin increased from 56.8 +/- 3.2% to 84.9 +/- 1.9% (p < 00.0001). These findings showed a clear improvement in the majority of the evaluated parameters in all of the patients, and, in many of them, a normalization. We conclude that the nasal CPAP is effective for the acute improvement of the disorders observed during the sleep in patients with OSA, which supports its therapeutic use in this illness.

Reconstruction of magnetic source images using the Wiener filter and a multichannel magnetic imaging system.

A system for imaging magnetic surfaces using a magnetoresistive sensor array is developed. The experimental setup is composed of a linear array of 12 sensors uniformly spaced, with sensitivity of 150 pT*Hz(-1/2) at 1 Hz, and it is able to scan an area of (16 × 18) cm(2) from a separation of 0.8 cm of the sources with a resolution of 0.3 cm. Moreover, the point spread function of the multi-sensor system is also studied, in order to characterize its transference function and to improve the quality in the restoration of images. Furthermore, the images are generated by mapping the response of the sensors due to the presence of phantoms constructed of iron oxide, which are magnetized by a pulse of 80 mT. The magnetized phantoms are linearly scanned through the sensor array and the remanent magnetic field is acquired and displayed in gray levels using a PC. The images of the magnetic sources are reconstructed using two-dimensional generalized parametric Wiener filtering. Our results exhibit a very good capability to determine the spatial distribution of magnetic field sources, which produce magnetic fields of low intensity.

Superficial magnetic imaging by an xy-scanner of three magnetoresistive channels.

A scanning system developed for planar magnetic surfaces composed of a moving line of three magnetoresistive ultrasensitive transducers, complemented by a signal conditioning circuit is presented. After the calibration of the sensors, it was used to determine magnetized surface images with different shapes to evaluate the sensitivity of the device, and the images are represented in gray levels on a scale from 0 to 255 intensities, to get a visual representation of the magnetic field strength. The device is shown to be sensitive enough to detect gradients homogeneities and discontinuities in the magnetic field maps and images of magnetic susceptibility.

An induction heater device for studies of magnetic hyperthermia and specific absorption ratio measurements.

The development of a device for generating ac magnetic fields based on a resonant inverter is presented, which has been specially designed to carry out experiments of magnetic hyperthermia. By determining the electric current in the LC resonant circuit, a maximum intensity of magnetic field around of 15 mT is calculated, with a frequency around of 206 kHz. This ac magnetic field is able to heat powdered magnetic materials embedded in biological systems to be used in biomedical applications. Indeed, in order to evaluate the sensitivity of the device we also present the measurements of the specific absorption rate in phantoms performed with commercially prepared Fe(3)O(4) and distilled water at different concentrations.

Mutant prevention concentrations of fluoroquinolones for Enterobacteriaceae expressing the plasmid-carried quinolone resistance determinant qnrA1.

The influence of qnrA1 on the development of quinolone resistance in Enterobacteriaceae was evaluated by using the mutant prevention concentration parameter. The expression of qnrA1 considerably increased the mutant prevention concentration compared to strains without this gene. In the presence of qnrA1, mutations in gyrA and parC genes were easily selected to produce high levels of quinolone resistance.

[Microbial interactions with heavy metals]. / Interacciones microbianas con metales pesados.

Living organisms are exposed in nature to heavy metals, commonly present in their ionized species. These ions exert diverse toxic effects on microorganisms. Metal exposure both selects and maintains microbial variants able to tolerate their harmful effects. Varied and efficient metal resistance mechanisms have been identified in diverse species of bacteria, fungi and protists. The study of the interactions between microorganisms and metals may be helpful to understand the relations of toxic metals with higher organisms such as mammals and plants. Some microbial systems of metal tolerance have the potential to be used in biotechnological processes, such as the bioremediation of environmental metal pollution or the recovery of valuable metals. In this work we analyze several examples of the interactions of different types of microbes with heavy metals; these cases are related either with basic research or with possible practical applications.

Analgesia postoperatoria en la queiloplastia del lactante. Estudio comparativo: bloqueo infraorbitario intraoral bilateral con bupivacaína 0, 25% con adrenalina vs. analgesia intravenosa con tramadol / Postoperative analgesia for the management of chieloplasty in the breast-fed baby. Comparative study: bilateral intraoral blockade of the infraorbitary nerve with bupivacaine 0.25% plus adrenaline versus intravenous analgesia with tramadol

Objetivo: Comparar la eficacia y duración del bloqueo del nervio infraorbitario intraoral bilateral frente a la analgesia intravenosa convencional con tramadol en el control del dolor postoperatorio en lactantes sometidos a queiloplastia por labio leporino. Material y métodos: Tras la realización de una adecuada valoración preanestésica y la obtención del consentimiento informado de los padres, realizamos un estudio prospectivo, controlado aleatorizado y doble ciego en 25 niños, ASA I, con edades comprendidas entre los 3-10 meses, tras obtener el consentimiento informado de los padres, propuestos para cirugía correctora de labio leporino (queiloplastia). Todos ellos fueron premeditados, media hora antes de la intervención quirúrgica, con midazolan oral (0,5 mg.kg-1) y, en todos los casos, se practicó la técnica anestésica y el bloqueo nervioso por el mismo anestesiólogo, que consistió en una inducción inhalatoria con sevoflurano previa a la venoclisis. La anestesia general se completó con atropina, fentanilo y rocuronio a las dosis establecidas que se administraron, por vía intravenosa, previamente a la intubación endotraqueal y la ventilación mecánica. Los pacientes fueron divididos aleatoriamente en dos grupos: Grupo A (n = 12): se administró 1-2 ml de bupivacaína al 0,25% con adrenalina para el bloqueo infraorbitario bilateral y solución salina intravenosa como sustitutivo de la analgesia intravenosa con tramadol. Grupo B (n = 13): se administró solución salina para el bloqueo nervioso, en lugar de la bupivacaína, y tramadol intravenoso (1,5 mg.kg-1) como analgesia postoperatoria. En todos ellos se realizó anestesia general con sevoflurano y fentanilo "a demanda" según parámetros convencionales (tensión arterial, frecuencia cardiaca, tamaño pupilar, etc.). Durante sus primeras seis horas de estancia en Reanimación se valoró la duración de la analgesia, grado de disconfort e intensidad dolorosa. Además se anotó la aparición de cualquier complicación o efecto adverso. Los datos se analizaron mediante t Student y test χ2. Resultados: No existieron diferencias en cuanto a las características demográficas. La duración de la analgesia fue mayor en el grupo A (7,3 ± 5,1 h) que en el grupo B (2,8 ± 2,2 h) (p < 0,01). La valoración subjetiva de la intensidad dolorosa fue mayor en el grupo B (2,23 ± 0,83) que en el grupo A (0,66 ± 0,6) (p < 0,01). El grado de confort, según escala objetiva, fue significativamente mayor en el grupo que recibió el bloqueo con anestésico local. Discusión: La anestesia para neonatos sometidos a reparación de labio leporino, empleando bloqueo bilateral intraoral del nervio infraorbitario es una técnica segura, simple y rápida, que permite una analgesia duradera en el postoperatorio a la vez que minimiza los riesgos de depresión respiratoria, ya que posibilita una reducción en la utilización de analgésicos opioides, permitiendo un inmediato y confortable despertar (AU)

Objective: To compare the effectiveness and length of bilateral intraoral blockade of the infraorbitary nerve versus standard intravenous analgesia with tramadol for the management of postoperative pain in breast-fed infants undergoing chieloplasty due to harelip. Material and methods: After conducting an adequate pre-anesthetic assessment and obtaining the informed consent from their parents, we performed a double-blind, randomized, controlled and randomized study in 25 ASA I infants with ages ranging from 3 to 10 months that were candidates to corrective harelip surgery (chieloplasty). All of them were pretreated half an hour before the surgical procedure with oral midazolan (0.5 mg.kg-1) and the same anesthesiologist performed the anesthetic technique and the nerve blockade in all the cases, this being inhaled induction with sevoflurane prior to venoclysis. General anesthesia was achieved with intravenous administration of atropine, fentanyl and rocuronium at the standard doses prior to endotracheal intubation and mechanical ventilation. Patients were randomized to one of the following groups: Group A (n = 12): 1-2 ml of bupivacaine 0.25% plus adrenaline was administered for bilateral blockade of the intraorbitary nerve and intravenous saline solution instead of intravenous analgesia with tramadol. Group B (n = 13): saline solution was administered for nerve blockade, instead of bupivacaine, and intravenous tramadol (1.5 mg.kg-1) was provided as postoperative analgesia. All of the patients underwent general anesthesia with sevoflurane and fentanyl "on-demand" according to standard parameters (blood pressure, heart rate, pupil size, etc.). During the first six hours at Reanimation, length of analgesia, degree of discomfort and pain severity were assessed. The presence of complications or side effects was also recorded. Data were analyzed using the t Student and the χ2 test. Results: No differences were found regarding demographic features. Analgesia lasted more in group A (7.3 ± 5.1 h) compared to group B (2.8 ± 2.2 h) (p < 0.01). Subjective assessment of pain severity was higher in group B (2.23 ± 0.83) compared to group A (0.66 ± 0.6) (p < 0.01). Degree of comfort, as assessed through an objective scale, was significantly greater in the group undergoing blockade with local anesthetic. Discussion: Anesthesia for neonates undergoing surgical reparation of harelip with intraoral bilateral blockade of the infraorbitary nerve is a safe, simple and quick technique that provides a lasting postoperative analgesia and also reduces the risks of respiratory depression, since it allows a lower use of opiate analgesics and, hence, an immediate and comfortable awakening (AU)