Design and Synthesis of Cyclic Dinucleotide Analogues Containing Triazolyl C-Nucleosides.

Natural cyclic dinucleotide (CDN) is the secondary messenger involved in bacterial hemostasis, human innate immunity, and bacterial antiphage immunity. Synthetic CDN and its analogues are key molecular probes and potential immunotherapeutic agents. Several CDN analogues are under clinical research for antitumor immunotherapy. A myriad of synthetic methods have been developed and reported for the preparation of CDN and its analogues. However, most of the protocols require multiple steps, and only one CDN or its analogue is prepared at a time. In this study, a strategy based on a macrocyclic ribose phosphate skeleton containing a 1'-alkynyl group was designed and developed to prepare CDN analogues containing triazolyl C-nucleosides by click chemistry. Combinatorial application of click chemistry and the sulfenylation cascade to the macrocyclic skeleton expanded the diversity of the CDN analogues. This macrocyclic skeleton strategy rapidly and efficiently provides CDN analogues to facilitate research on microbiology, immunology, and immunotherapy.

Typical Tire Additives in River Water: Leaching, Transformation, and Environmental Risk Assessment.

Tire wear particles (TWPs) released during vehicle driving can enter water bodies, leading to leaching of tire additives (TAs) in aquatic environments. However, the transformation behavior and related ecological impacts of TAs and their transformation products (TPs) remain unclear. In this study, laboratory-based simulation experiments and field investigations were conducted to explore the transformation mechanisms and ecological risks of TAs. After being placed in river water for 24 h, about 7-95% of 12 investigated TAs in TWPs were leached. Forty-eight TPs from eight TAs were tentatively identified along with different transformation pathways via suspect screening by high-resolution mass spectrometry. Semiquantitative results indicated that TPs derived from N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylene-diamine (6PPD) were predominant in leachates, while aryl hydrolysis and quinone pathways were the main transformation pathways. Field investigations on urban surface water samples from 16 sites in Hong Kong revealed the occurrence of 17 TAs and 1 TP, with concentrations ranging from 13.9 to 2230 ng/L (median ± standard deviation: 226 ± 534 ng/L). Sixteen TPs from six TAs were additionally identified via suspect screening. It is estimated that 6PPD-quinone and seven TAs could pose medium to high ecological risk, while N-(1,3-dimethylbutyl)-N'-phenyl-p-quinonediimine, a frequently detected TP, was identified as a persistent-bioaccumulative-toxic substance.

Porter effect vs cost effect: The impact of China's low carbon city pilot on carbon emissions and economic performance.

Building a low-carbon economy can help cities effectively mitigate climate risks, but it is challenging for developing countries. Using a difference-in-difference and event study model, we investigate the joint impact of China's Low-Carbon City Pilot (LCCP) on carbon emissions and economic performance. Our findings show that the LCCP significantly reduces carbon emissions and increases gross revenues, employee count, and fixed assets without compromising the net profit of manufacturing firms. The LCCP has a cumulative effect, with the positive joint impact increasing gradually over time. A heterogeneity analysis shows that the later pilot cities have not achieved better carbon emissions and economic performance than the early pilot cities. The reason for the positive joint effect of LCCP is that the Porter effect outweighs the cost effect. These findings contribute to knowledge about how developing countries can develop a low-carbon economy.

Positive Charge Holes Revealed by Energy Band Theory in Multiphase Tix O2x-1 and Exploration of its Microscopic Electromagnetic Loss Mechanism.

Although numerous experimental investigations have been carried out on the problem of defect engineering in semiconductor absorbers, the relationship among charge carrier, defects, heterointerfaces, and electromagnetic (EM) wave absorption has not been established systematically. Herein, the new thermodynamic and kinetic control strategy is proposed to establish multiphase Tix O2 x -1 (1 ≤ x ≤ 6) through a hydrogenation calcination. The TiOC-900 composite shows the efficient EM wave absorption capability with a minimum reflection loss (RLmin ) of -69.6 dB at a thickness of 2.04 mm corresponding to an effective absorption bandwidth (EAB) of 4.0 GHz due to the holes induced conductance loss and heterointerfaces induced interfacial polarization. Benefiting from the controllable preparation of multiphase Tix O2 x -1 , a new pathway is proposed for designing high-efficiency EM wave absorbing semiconducting oxides. The validity of the method for adopting energy band theory to explore the underlying relations among charge carriers, defects, heterointerfaces, and EM properties in multiphase Tix O2 x -1 is demonstrated for the first time, which is of great importance in optimizing the EM wave absorption performance by electronic structure tailoring.

New Perspective to Understand and Prioritize the Ecological Impacts of Organophosphate Esters and Transformation Products in Urban Stormwater and Sewage Effluents.

Due to their prevalence in urban contaminated water, the driving factors of organophosphate esters (OPEs) need to be well examined, and their related ecological impacts should include that of their transformation products (TPs). Additionally, a robust framework needs to be developed to integrate multiple variables related to ecological impacts for improving the ecological health assessment. Therefore, OPEs and TPs in urban stormwater and wastewater in Hong Kong were analyzed to fill these gaps. The results revealed that the total concentrations of OPEs in stormwater were positively correlated with the area of transportation land. Individual TP concentrations and the mass ratios of individual TPs/OPEs were somewhat higher in sewage effluents than that in stormwater. OPEs generally showed relatively higher risk quotients than TPs; however, the total risk quotients increased by approximately 38% when TPs were factored in. Moreover, the molecular docking results suggested that the investigated TPs might cause similar endocrine disruption in marine organisms as their parent OPEs. This study employed the Toxicological-Priority-Index scheme to successfully integrate the ecological risks and endocrine-disrupting effects to refine the ecological health assessment of the exposure to OPEs and their TPs, which can better inform the authority on the prioritization for regulating these contaminants of emerging concern in urban built environments.

Does air pollution inhibit manufacturing productivity in Yangtze River Delta, China? Moderating effects of temperature.

China has been experiencing serious and recurrent incidences of air pollution in recent years. The frequency and timespans of such incidences are uncertain because of variable urban weather conditions, especially temperature, that inhibit the productivity of manufacturing companies. Matching data about listed manufacturing companies in China's Yangtze River Delta urban cluster from 2003 to 2018 with data on urban air pollution and weather, we explored the effects of air pollution on corporate productivity and the moderating role of temperature. We found that air pollution significantly inhibited the productivity of these companies, which decreased by about 0.1% for 1% increase in the concentration of PM2.5. Regarding industry heterogeneity, high-energy-consumption and low-technology manufacturing industries were more sensitive to the negative effects of air pollution. Furthermore, we concluded that low temperatures played an important role in causing significant increases in the negative effects of air pollution. In the fall and winter (October to January), the lower the temperatures resulted in stronger inhibitory effects of air pollution on corporate productivity. When the average daily temperature is 0°C-3°C, the moderating effects of temperature are even more obvious. To minimize the inhibitory effects of air pollution on productivity, governments and companies should implement positive adaptions to simultaneously confront air pollution and temperature change.

Development and Evaluation of a Water-in-oil Microemulsion Formulation for the Transdermal Drug Delivery of Teriflunomide (A771726).

Teriflunomide (TEF, A771726) is the active metabolite of leflunomide (LEF), a disease-modifying anti-rheumatic drug. The main purpose of this study was to develop and evaluate water-in-oil (W/O) microemulsion formulation of TEF. The W/O microemulsion was optimized formula is the physical and chemical stability of lecithin, ethanol, isopropyl myristate (IPM) and water (20.65/20.78/41.52/17.05 w/w) by using the pseudo-ternary phase diagram and the average droplet size is about 40 nm. The permeability of TEF microemulsion is about 6 times higher than control group in vitro penetration test. The results of anti-inflammatory effect showed that compared with the control group, the external TEF microemulsion group could significantly inhibit swelling of paw in rats, and no significant difference compared with oral LEF group. The results of hepatotoxicity test show that there were normal content of alanine aminotransferase (ALT)/aspartate aminotransferase (AST) and no obvious inflammatory infiltration of TEF microemulsion group compared with LEF group. The plasma concentration curve showed that compared with LEF group, the peak concentration of TEF microemulsion group was decreased, the half-life (t1/2) was prolonged, and the relative bioavailability of TEF microemulsion was 75.35%. These results suggest that TEF W/O microemulsion can be used as a promising preparation to play an anti-inflammatory role while significantly reducing hepatotoxicity.

Feasibility of nanoscale zero-valent iron to enhance the removal efficiencies of heavy metals from polluted soils by organic acids.

Soil washing with natural chelators to remediate metal-contaminated soils has been gained attention by researchers. However, the abilities of the chelators to remediate the multiple metal polluted soils are less effective. This study employed zero-valent iron nanoparticle (nZVI) to enhance the removal efficiencies of citric (CA), tartaric (TA) and oxalic acids (OA), and evaluate their feasibility. Results showed that metal removal efficiencies increased with the increasing concentration of nZVI and soil-liquid ratio, decreased with the increasing solution pH. The kinetic simulation indicated that pseudo-first-order and pseudo-second-order models could be used for describing the washing processes. Additionally, metal removals were significantly improved by addition of nZVI (p < 0.05). The highest enhancements of soil Cd, Pb and Zn removals under solution pH of 4.0, soil-liquid ratio of 1:20 and washing time of 120 min reached 12.83% (OA- nZVI), 24.92% (CA-nZVI) and 11.64% (OA- nZVI) for mine soil, and 19.24% (TA- nZVI), 18.16% (CA-nZVI) and 8.93% (OA- nZVI) for farmland soil, respectively. After soil washing, the exchangeable forms and the environmental risks of residual metals were markedly diminished in soils. Therefore, the combinations of the organic acids and nZVI are the feasible practices to repair the soils contaminated by heavy metals.

Melatonin improves the storage quality of rabbiteye blueberry (Vaccinium ashei) by affecting cuticular wax profile.

Cuticular wax is the first line of structural defense for plants against external stresses. This study investigated the effects of melatonin (MT) on chemical composition and accumulation profile of wax, as well as fruit quality of rabbiteye blueberry during storage. The results indicated a significant reduction in the overall wax content during storage. Nevertheless, MT effectively delayed the decline, with a higher amount of 9.8% and 15.17% in the treated 'Baldwin' and 'Garden Blue' compared to their respective controls at 21st day of storage. The wax composition significantly varied depending on storage time, MT treatment, and cultivars. Additionally, MT markedly improved the fruit quality of rabbiteye blueberries. Correlation analysis revealed water loss and decay rates were negatively correlated with triterpenoids and fatty acids. Taken together, this study highlights the positive effects of post-harvest MT application on shelf life and fruit quality of blueberry by modifying the wax profile during storage.

Microplastics in Hong Kong's marine waters: Impact of rainfall and Pearl River discharge.

This study provided a systematic investigation of microplastics in Hong Kong's surface marine waters during the pandemic from 2019 to 2021. Microplastics (2.07 ± 4.00 particles/m3) exhibited significant temporal variations with higher abundance in the wet season, without a consistent trend after the mandatory mask-wearing requirement was announced. The impact of pandemic restrictions on microplastic distribution was found to be relatively minor. However, significant correlations between microplastic abundances and rainfall highlighted the substantial contribution of local emissions through surface runoff. Notably, sites in closer proximity to the Pearl River Delta exhibited higher microplastic abundances, indicating their association with emission sources. The influence of rainfall and adverse weather on marine microplastic loads demonstrated different sensitivities among various locations but can generally last for one month. These results revealed the impact of seasonal rainfall on coastal microplastics and emphasized the need for efforts to reduce microplastic discharge from land-based sources.

3D autofluorescence imaging of hydronephrosis and renal anatomical structure using cryo-micro-optical sectioning tomography.

Rationale: Mesoscopic visualization of the main anatomical structures of the whole kidney in vivo plays an important role in the pathological diagnosis and exploration of the etiology of hydronephrosis. However, traditional imaging methods cannot achieve whole-kidney imaging with micron resolution under conditions representing in vivo perfusion. Methods: We used in vivo cryofixation (IVCF) to fix acute obstructive hydronephrosis (unilateral ureteral obstruction, UUO), chronic spontaneous hydronephrosis (db/db mice), and their control mouse kidneys for cryo-micro-optical sectioning tomography (cryo-MOST) autofluorescence imaging. We quantitatively assessed the kidney-wide pathological changes in the main anatomical structures, including hydronephrosis, renal subregions, arteries, veins, glomeruli, renal tubules, and peritubular functional capillaries. Results: By comparison with microcomputed tomography imaging, we confirmed that IVCF can maintain the status of the kidney in vivo. Cryo-MOST autofluorescence imaging can display the main renal anatomical structures with a cellular resolution without contrast agents. The hydronephrosis volume reached 26.11 ± 6.00 mm3 and 13.01 ± 3.74 mm3 in 3 days after UUO and in 15-week-old db/db mouse kidneys, respectively. The volume of the cortex and inner stripe of the outer medulla (ISOM) increased while that of the inner medulla (IM) decreased in UUO mouse kidneys. Db/db mice also showed an increase in the volume of the cortex and ISOM volume but no atrophy in the IM. The diameter of the proximal convoluted tubule and proximal straight tubule increased in both UUO and db/db mouse kidneys, indicating that proximal tubules were damaged. However, some renal tubules showed abnormal central bulge highlighting in the UUO mice, but the morphology of renal tubules was normal in the db/db mice, suggesting differences in the pathology and severity of hydronephrosis between the two models. UUO mouse kidneys also showed vascular damage, including segmental artery and vein atrophy and arcuate vein dilation, and the density of peritubular functional capillaries in the cortex and IM was reduced by 37.2% and 49.5%, respectively, suggesting renal hypoxia. In contrast, db/db mouse kidneys showed a normal vascular morphology and peritubular functional capillary density. Finally, we found that the db/db mice displayed vesicoureteral reflux and bladder overactivity, which may be the cause of hydronephrosis formation. Conclusions: We observed and compared main renal structural changes in hydronephrosis under conditions representing in vivo perfusion in UUO, db/db, and control mice through cryo-MOST autofluorescence imaging. The results indicate that cryo-MOST with IVCF can serve as a simple and powerful tool to quantitatively evaluate the in vivo pathological changes in three dimensions, especially the distribution of body fluids in the whole kidney. This method is potentially applicable to the three-dimensional visualization of other tissues, organs, and even the whole body, which may provide new insights into pathological changes in diseases.

Embryo developmental toxicity in marine medaka (Oryzias melastigma) due to parental and embryonic 17α-ethinylestradiol exposure.

The synthetic estrogen 17α-ethinylestradiol (EE2) is a common component of hormone therapy and oral contraceptives and has been widely used for nearly 60 years. Numerous studies have shown that exposure to EE2 can affect embryonic development in a number of fish species. The effects of parental and embryonic EE2 exposure on embryo developmental toxicity and the underlying molecular mechanisms, however, have rarely been examined. In this study, embryos collected from parental EE2-exposed adult fish were examined to assess EE2-induecd toxicity during embryo development. The rate of embryo development including heart rate, hatching rate, and larval locomotion were measured to assess embryo developmental toxicity. The embryonic transcriptome was used to delineate the related developmental toxicity pathways. Our results suggest that parental and embryonic EE2 exposure resulted in growth retardation including a reduction in embryo heart rate, a delay in the appearance eye pigmentation, decreased hatching rate and impaired larval locomotion. In addition, gene ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and Ingenuity Pathway Analysis (IPA) of transcriptome revealed that these impairments are controlled by estrogen receptor and related to eye structure, neuronal and synaptic structure, and behaviour. The key factors identified, including PRKAA2, APOB, EPHB2, OXTR, NR2E3, and POU4F2, could serve as biomarkers for assessing EE2-induced embryo developmental toxicity. For the first time, our results show that eye pigmentation is a potentially sensitive marker of EE2-induced embryo developmental toxicity.

Gut microbiota and integrative traditional Chinese and western medicine in prevention and treatment of heart failure.

BACKGROUND: Heart failure (HF) is the terminal stage of multiple cardiovascular diseases, with high mortality and morbidity. More and more studies have proved that gut microbiota may play a role in the process of HF, which is expected to become a new therapeutic target. The combination of traditional Chinese and Western medicine has vast therapeutic potential of complementation against HF. PURPOSE: This manuscript expounds on the research progress of mechanisms of gut microbiota participating in the occurrence and prognosis of HF and the role of integrative traditional Chinese and Western medicine from 1987 to 2022. The combination of traditional Chinese and Western medicine in the prevention and treatment of HF from the perspective of gut microbiota has been discussed. METHODS: Studies focusing on the effects and their mechanisms of gut microbiota in HF and the role of integrative traditional Chinese and Western medicine were identified and summarized, including contributions from February 1987 until August 2022. The investigation was carried out in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. We searched PubMed, Embase, Cochrane Library, CNKI, Wanfang, and VIP databases up to April 2023 by using the relevant keywords and operators. RESULTS: A total of 34 articles were finally included in this review.16 RCTs and 13 basic researches, and 3 clinical research studies involving 7 relevant outcome indicators(cardiac function evaluation index, changes in gut microbiota, inflammatory factors, metabolites of gut microbiota, serum nutritional index protein, quality of life score, intestinal permeability and all-cause mortality). Compared with healthy controls, serum TNF-α and TMAO levels were significantly higher in patients with heart failure [MD = 5.77, 95%CI(4.97, 6.56), p < 0.0001; SMD = 1.92, 95%CI(1.70, 2.14), p < 0.0001]. Escherichia coli and Thick-walled bacteria increased significantly [SMD = -0.99, 95%CI(-1.38, -0.61), p < 0.0001, SMD = 2.58, 95%CI(2.23, 2.93), p < 0.0001];The number of bacteroides and lactobacillus decreased [SMD = -2.29, 95%CI(-2.54, -2.04), p < 0.0001; SMD = -1.55, 95%CI(-1.8, -1.3), p < 0.0001]. There was no difference in bifidobacterium [SMD = 0.16, 95%CI(-0.22, 0.54), p = 0.42]. In the published literature, it is not difficult to see that most of the results are studied and proved based on animal experiments or clinical trials, involving the cellular level, while the mechanism and mode of action of the molecular biology of traditional Chinese medicine are less elaborated, which is related to the characteristics of multi-components and multi-targets of traditional Chinese medicine. The above are the shortcomings of published literature, which can also be the direction of future research. CONCLUSION: Heart failure patients have decreased beneficial bacteria such as Bacillus mimics and Lactobacillus in the intestinal flora and increased harmful flora like thick-walled flora. And increase the inflammatory response of the body and the expression of trimethylamine oxide (TMAO) in the serum. And The prevention and treatment of integrative traditional Chinese and Western medicine against heart failure based on gut microbiota and its metabolites is a promising research direction.

Microplastic occurrence and ecological risk assessment in the eight outlets of the Pearl River Estuary, a new insight into the riverine microplastic input to the northern South China Sea.

Estuaries are unique transition zones connecting terrestrial and coastal environments and are recognized as primary conveyors for land-derived plastics to open oceans. Riverine microplastics (MPs) have been commonly investigated using sequential sampling which might not effectively reflect the actual load. In this study, sampling at eight outlets was performed during a complete tidal cycle to estimate the MP flux to the Pearl River Estuarine (PRE) using a concurrent sampling strategy. The MP abundances ranged from 2.90 ± 0.57-5.9 ± 2.27 particles/L. A remarkable difference between tides in MP abundances suggests tidal effect should not be overlooked in assessment. The MP load through the eight outlets was estimated at 304 trillion particles or 1102 tons into the PRE annually. Additionally, similar potential ecological risk assessment among eight rivers implied that environmental threats posed by less urbanized and populated rural areas on the western side have been under-evaluating for decades.

Microplastics: A major source of phthalate esters in aquatic environments.

Phthalate esters (PAEs) are predominant additives in plastics, their widespread contamination in aquatic environments has raised global concern. Here, twelve plastic products were prepared as microplastics to investigate their release behaviors of PAEs. Six out of 15 PAEs were quantified after 14 days of incubation in water. The leaching potentials were plastic type-specific, where the pencil case (polyvinyl chloride, PVC) represented the highest migrations with total ∑15 PAEs concentration of 6660 ± 513 ng/g, followed by the cleaning brush-1 (polyamide, PA, ~1830 ng/g) and rubber glove (1390 ± 57.5 ng/g). Conversely, the straw (polypropylene, PP), cleaning brush-2 (polyethylene terephthalate, PET) and shampoo bottle (PET) released the lowest amounts of PAEs, with 50.3 ± 8.21, 93.9 ± 91.8 and 104.35 ng/g, respectively. The release patterns of PAE congeners were polymer type-related, where di-n-butyl phthalate (DBP) dominated the leaching from PA, PP and PET microplastics (47-84%), diethyl phthalate leached the most from PVC and rubber microplastics (45-92%), while diisobutyl phthalate and DBP dominated the leaching from PE microplastics (68-94%). Water chemical properties could affect PAEs migration and the kinetic leaching process was well fitted with the pseudo-first-order model. Approximately 57.8-16,100 kg/year of PAEs were estimated to be released into oceans from microplastics.

Microplastic occurrence in the northern South China Sea, A case for Pre and Post cyclone analysis.

Microplastics (MPs) have become a great concern owing to their persistence and ecotoxicity in various environmental compartments. MPs can be transported from land to oceans via the aquatic system, and the oceans are believed to be the final sink for MPs. To resolve the lack of data concerning MP contamination in the northern South China Sea (NSCS), coastal seawater and sediments were investigated. The sample sites included the coastal area from Hong Kong to western Guangdong Province, which provided a representative coverage of variable distance to the shore. MP particles were observed in all samples, suggesting a widespread MP pollution in NSCS. The average MP abundance was relatively high in South China Sea, but it was lower than that in other Chinese coastal areas. In all samples, the dominant MP polymer was polyethylene terephthalate fibers. No statistically significant correlation was found between the abundances of MPs in seawater and sediment. The MP distribution in surface water affected by the monsoon exhibited high abundance in the east and low in the west, and MPs were more abundant near the shore than away from it. The tropical cyclone had no obvious effect on the overall MP abundance except in the Hong Kong west marine water (R4), which may attribute to proximity to Hong Kong and other islands.

Melatonin affects cuticular wax profile in rabbiteye blueberry (Vaccinium ashei) during fruit development.

Cuticular wax has been implicated in the first line of plant defense mechanism against external stresses. In this study, cuticular wax on the fruits of two rabbiteye blueberry cultivars cv. Baldwin and Brightwell were examined in terms of the morphology and chemical composition during fruit development in the presence and absence of pre-harvest melatonin (MT) treatment. It revealed that the crystal wax appeared as numerous tubules, and its morphology was not affected by MT treatment. A dominant constituent of triterpenoid at veraison stage was ß-amyrin (45.62% of total wax) in Baldwin and oleanolic acid (22.79% of total wax) in Brightwell. Exogenous MT application significantly promoted fruit quality and increased total content of cuticular wax, however, the effect of MT on individual wax components greatly varied depending on cultivars and fruit developmental stage. Consequently, MT application may enhance sustainability of post-harvest fruit production systems by facilitating accumulation of cuticular wax.

Significant riverine inputs of typical plastic additives-phthalate esters from the Pearl River Delta to the northern South China Sea.

Phthalate esters (PAEs) are representative additives used extensively in plastics. In this study, 15 PAEs were investigated at the eight riverine outlets of the Pearl River Delta (PRD). The total concentrations of Σ15PAEs, including both the dissolved and particulate phases, ranged from 562 to 1460 ng/L and 679 ng/L-2830 ng/L in the surface and bottom layers, respectively. Dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP) dominated in the dissolved and suspended particulate matter (SPM) phases, respectively, accounting for >50 % and > 80 % of Σ15PAEs. Riverine input of wastewater from the PRD was possibly the primary source of the contamination. Higher levels of PAEs occurred at the eastern outlets than at the western ones. The dissolved and particulate PAEs varied seasonally, with significantly higher concentrations observed in the dry season than in the wet season. However, no significant differences of PAE levels in both phases were observed among low, medium, and high tides. The partitioning results demonstrated that SPM is important in the transportation of pollutants in estuaries, where more hydrophobic DEHP was predominantly transported by the SPM phase, while those more hydrophilic ones were regularly transported by the dissolved phase. The total annual flux of Σ15PAEs through the eight outlets to the SCS reached 1390 tons.

Spatiotemporal occurrence of phthalate esters in stormwater drains of Hong Kong, China: Mass loading and source identification.

Urban stormwater is an important pathway for transporting anthropogenic pollutants to water bodies. Phthalate esters (PAEs) are endocrine disruptors owing to their estrogenic activity and potential carcinogenicity and their ubiquitous presence has garnered global interest. However, their transportation by urban stormwater has been largely overlooked. This study, for the first time, investigated 15 PAEs in stormwater from six major stormwater drains in the highly urbanized Hong Kong, a major metropolitan city in China. The results showed that PAEs were ubiquitous in the stormwater of Hong Kong, with total concentrations (∑15PAEs) spanning from 195 to 80,500 ng/L. Bis(2-n-butoxyethyl) phthalate (DBEP), diisopentyl phthalate (DiPP), dicyclohexyl phthalate (DCHP) and di-n-pentyl phthalate (DnPP) were detected in stormwater for the first time. Spatial variations in PAEs were observed among different stormwater drains, possibly due to the different land use patterns and intensities of human activities in their respective catchments. The highest and lowest levels of ∑15PAEs were found in Kwai Chung (3860 ± 1960 ng/L) and the Ng Tung River (672 ± 557 ng/L), respectively. Additionally, significantly higher concentrations of ∑15PAEs in stormwater were found in the wet season (2520 ± 2050 ng/L) than in the dry season (947 ± 904 ng/L). Principal component analysis classified domestic and industrial origins as two important sources of PAEs in the stormwater of Hong Kong. Stormwater played a crucial role in transporting PAEs, with an estimated annual flux of 0.705-29.4 kg. Thus, possible stormwater management measures were proposed to protect the receiving environment and local ecosystems from stormwater.

Phthalate esters in seawater and sediment of the northern South China Sea: Occurrence, distribution, and ecological risks.

In this study, the occurrence and distribution of 15 phthalate esters (PAEs) in seawater and sediment from the northern South China Sea (NSCS) were investigated for the first time to improve understanding on the contamination status of PAEs in this region. The concentrations of total PAEs (∑15 PAEs) were found to range from 68.8 to 1500 ng/L, 46.0 to 7800 ng/L, and 49.2 to 440 ng/g dry weight in surface seawater, bottom seawater, and sediment, respectively. Among the 15 PAEs, dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) were the predominant PAE congeners, with mean contributions of 44.7% and 24.0% in surface water, and 42.7% and 25.8% in bottom water, respectively. Moreover, diisobutyl phthalate (DiBP) constituted the majority of ∑15 PAEs in the sediment (61.3%). Comparatively high concentrations of Σ15 PAEs were observed in seawater at the sites within the western NSCS, whereas relatively higher concentrations of Σ15 PAEs were detected in sediments at the eastern NSCS. River input and atmospheric deposition could be the main sources of PAEs in the NSCS. Preliminary risk assessment implied that DBP, DiBP, and DEHP posed low to high potential risks for marine organisms at different trophic levels. These results would be valuable for implementing effective control measures and remediation strategies for PAEs contamination in the region.