The complexity of female orgasm and ejaculation.

The anatomy and physiology of the female orgasm are often neglected. The female orgasm is a normal psychophysiological function to all women, and some even can achieve ejaculation as part of the normal physiological response at the height of sexual arousal. The complexity of female sexuality requires a deep understanding of genital anatomy. The clitoris is the principal organ for female pleasure. The vaginal stimulation of the anterior vaginal wall led women to orgasm due to the stimulation of the clitourethrovaginal complex and not due to stimulation of a particular organ called the G spot in the anterior distal vaginal wall. Female ejaculation follows orgasm. It consists of the orgasmic expulsion of a smaller quantity of whitish fluid produced by the female prostate. Squirting can be differentiated from female ejaculation because it is the orgasmic transurethral expulsion of a substantial amount of diluted urine during sexual activity, and it is not considered pathological. The female orgasm is influenced by many aspects such as communication, emotional intimacy, long-standing relationship, adequate body image and self-esteem, proper touching and knowledge of the female body, regular masturbation, male sexual performance, male and female fertility, chronic pain, and capacity to engage in new sexual acts. Stronger orgasms could be achieved when clitoral stimulation, anterior vaginal wall stimulation, and oral sex is involved in the same sexual act.

MiR-144 overexpression as a promising therapeutic strategy to overcome glioblastoma cell invasiveness and resistance to chemotherapy.

Glioblastoma (GB) is the most aggressive and common form of primary brain tumor, characterized by fast proliferation, high invasion, and resistance to current standard treatment. The average survival rate post-diagnosis is only of 14.6 months, despite the aggressive standard post-surgery treatment approaches of radiotherapy concomitant with chemotherapy with temozolomide. Altered cell metabolism has been identified as an emerging cancer hallmark, including in GB, thus offering a new target for cancer therapies. On the other hand, abnormal expression levels of miRNAs, key regulators of multiple molecular pathways, have been correlated with pathological manifestations of cancer, such as chemoresistance, proliferation, and resistance to apoptosis. In this work, we hypothesized that gene therapy based on modulation of a miRNA with aberrant expression in GB and predicted to target crucial metabolic enzymes might impair tumor cell metabolism. We found that the increase of miR-144 levels, shown to be downregulated in U87 and DBTRG human GB cell lines, as well as in GB tumor samples, promoted the downregulation of mRNA of enzymes involved in bioenergetic pathways, with consequent alterations in cell metabolism, impairment of migratory capacity, and sensitization of DBTRG cells to a chemotherapeutic drug, the dichloroacetate (DCA). Taken together, our findings provide evidence that the miR-144 plus DCA combined therapy holds promise to overcome GB-acquired chemoresistance, therefore deserving to be explored toward its potential application as a complementary therapeutic approach to the current treatment options for this type of brain tumor.

Actividad física y estilo de vida sedentario en adultos, cambios durante el confinamiento por la pandemia de Covid-19.

No disponible.

Bardet-Biedl Syndrome proteins regulate cilia disassembly during tissue maturation.

Primary cilia are conserved organelles that mediate cellular communication crucial for organogenesis and homeostasis in numerous tissues. The retinal pigment epithelium (RPE) is a ciliated monolayer in the eye that borders the retina and is vital for visual function. Maturation of the RPE is absolutely critical for visual function and the role of the primary cilium in this process has been largely ignored to date. We show that primary cilia are transiently present during RPE development and that as the RPE matures, primary cilia retract, and gene expression of ciliary disassembly components decline. We observe that ciliary-associated BBS proteins protect against HDAC6-mediated ciliary disassembly via their recruitment of Inversin to the base of the primary cilium. Inhibition of ciliary disassembly components was able to rescue ciliary length defects in BBS deficient cells. This consequently affects ciliary regulation of Wnt signaling. Our results shed light onto the mechanisms by which cilia-mediated signaling facilitates tissue maturation.

Arabinogalactan protein 31 (AGP31), a putative network-forming protein in Arabidopsis thaliana cell walls?

BACKGROUND AND AIMS: Arabinogalactan protein 31 (AGP31) is a remarkable plant cell-wall protein displaying a multi-domain organization unique in Arabidopsis thaliana: it comprises a predicted signal peptide (SP), a short AGP domain of seven amino acids, a His-stretch, a Pro-rich domain and a PAC (PRP-AGP containing Cys) domain. AGP31 displays different O-glycosylation patterns with arabinogalactans on the AGP domain and Hyp-O-Gal/Ara-rich motifs on the Pro-rich domain. AGP31 has been identified as an abundant protein in cell walls of etiolated hypocotyls, but its function has not been investigated thus far. Literature data suggest that AGP31 may interact with cell-wall components. The purpose of the present study was to identify AGP31 partners to gain new insight into its function in cell walls. METHODS: Nitrocellulose membranes were prepared by spotting different polysaccharides, which were either obtained commercially or extracted from cell walls of Arabidopsis thaliana and Brachypodium distachyon. After validation of the arrays, in vitro interaction assays were carried out by probing the membranes with purified native AGP31 or recombinant PAC-V5-6xHis. In addition, dynamic light scattering (DLS) analyses were carried out on an AGP31 purified fraction. KEY RESULTS: It was demonstrated that AGP31 interacts through its PAC domain with galactans that are branches of rhamnogalacturonan I. This is the first experimental evidence that a PAC domain, also found as an entire protein or a domain of AGP31 homologues, can bind carbohydrates. AGP31 was also found to bind methylesterified polygalacturonic acid, possibly through its His-stretch. Finally, AGP31 was able to interact with itself in vitro through its PAC domain. DLS data showed that AGP31 forms aggregates in solution, corroborating the hypothesis of an auto-assembly. CONCLUSIONS: These results allow the proposal of a model of interactions of AGP31 with different cell-wall components, in which AGP31 participates in complex supra-molecular scaffolds. Such scaffolds could contribute to the strengthening of cell walls of quickly growing organs such as etiolated hypocotyls.

Planetary health diet versus usual diet in adolescents. How do food and physical activity influence academic performance?

Introduction: Objective: to analyze the relationship of the similarity of planetary health diet and habitual diet with the practice of physical activity on the academic performance of adolescent high school students by gender. Method: 877 adolescents were evaluated. The diet is reviewed through the 24-hour reminder. Physical activity was classified according to type of activity, frequency, duration, and intensity using the APALQ questionnaire. The caloric intake of adolescents was compared with the proposal of the EAT-LANCET commission, and the relationship between physical activity and diet with academic performance was again established. Results: Mexican adolescents do not comply with the proposal of a planetary health diet. No relationship was found between physical activity and academic performance. On the other hand, animal proteins rich in fats are consumed doubly according to the recommendation; these and whole grains, both are inversely related to better academic performance. Conclusions: Mexican adolescents are far from consuming a planetary health diet. Physical activity was not related to academic performance, but there is an inverse relationship between fat-rich animal proteins and whole grains in relation to subjects that contribute to academic performance, which suggests that a healthy diet such as the planetary diet proposal could have an impact on environmental sustainability and favorably impact the academic performance of adolescents.

Introducción: Objetivo: analizar la relación de la similitud entre la dieta de salud planetaria y la dieta habitual con la práctica de actividad física sobre el rendimiento académico en adolescentes estudiantes de secundaria por género. Método: se evaluaron 877 adolescentes. La dieta se revisó a través del recordatorio de 24 horas. Se clasificó la actividad física según el tipo de actividad, frecuencia, duración e intensidad con el cuestionario APALQ. Se comparó la ingesta calórica de los adolescentes con la propuesta por la comisión EAT-LANCET y se establecieron relaciones entre la actividad física y la dieta con el rendimiento académico. Resultados: los adolescentes mexicanos no cumplen con la propuesta de una dieta de salud planetaria. No se encontró relación entre la actividad física y el rendimiento académico. Por otro lado, las proteínas animales ricas en grasas se consumen doblemente de acuerdo con la recomendación; estas y los cereales integrales, ambos se relacionan inversamente con un mejor rendimiento académico. Conclusiones: los adolescentes mexicanos están lejos de consumir una dieta planetaria saludable. La actividad física no se relacionó con el rendimiento académico, pero sí existe una relación inversa entre las proteínas animales ricas en grasas y los cereales integrales con relación a materias que contribuyen con el rendimiento académico, lo que sugiere que una alimentación saludable como la propuesta de la dieta planetaria podría tener impacto sobre la sostenibilidad ambiental e impactar favorablemente en el rendimiento académico de los adolescentes.

Intrauterine Transmission of Zika and Vertical Transfer of Neutralizing Antibodies Detected Immediately at Birth in Oaxaca, Mexico: An Analysis in the Context of Microcephaly.

Zika virus (ZIKV) can cause neurological issues in infants. To provide protection, neutralizing antibodies should be transferred from the mother to the infant. We conducted a study at the Hospital General de Pochutla, Oaxaca, Mexico. Samples were collected from mothers (blood and breast milk) and infants (saliva and dried blood spots) within the first 12 postnatal hours (December 2017 to February 2018) and tested for ZIKV total and neutralizing antibodies as well as ZIKV-PCR. Microcephaly was evaluated according to INTERGROWTH-21st standards. Maternal IgG seroprevalence was 28.4% with 10.4% active infection, while infant IgG seroprevalence was 5.5% with 2.4% active infection. There were two cases of virolactia, and 6.3% of the infant saliva samples tested positive for ZIKV. Additionally, 18.3% of the infants were in a cephalic perimeter percentile lower than 10 and had an association between microcephaly and serology or a PCR between 8.6 and 60.9%. The infant blood samples had neutralizing antibodies, indicating intrauterine protection. Microcephaly was correlated with serology or PCR, but in our study population, non-ZIKV factors may be involved as well. Low ZIKV infection values in breast milk mean that breastfeeding is safe in most of the mothers and infants of the endemic area studied.

Prevalence of Polymorphism and Post-Training Expression of ACTN3 (R/X) and ACE (I/D) Genes in CrossFit Athletes.

BACKGROUND: CrossFit is known as a functional fitness training high-intensity exercise to improve physical performance. The most studied polymorphisms are the ACTN3 R577X gene, known for speed, power, and strength, and ACE I/D, related to endurance and strength. The present investigation analyzed the effects of training on ACTN3 and ACE gene expression in CrossFit athletes for 12 weeks. METHODS: the studies included 18 athletes from the Rx category, where ACTN3 (RR, RX, XX) and ACE (II, ID, DD) characterization of genotypes and tests of maximum strength (NSCA), power (T-Force), and aerobic endurance (Course Navette) were performed. The technique used was the reverse transcription-quantitative PCR real-time polymerase chain reaction (RT-qPCR) for the relative expression analysis. RESULTS: the relative quantification (RQ) values for the ACTN3 gene increased their levels 2.3 times (p = 0.035), and for ACE, they increased 3.0 times (p = 0.049). CONCLUSIONS: there is an overexpression of the ACTN3 and ACE genes due to the effect of training for 12 weeks. Additionally, the correlation of the expression of the ACTN3 (p = 0.040) and ACE (p = 0.030) genes with power was verified.

Impact of Adding a Rapid PCR-Based Blood Culture Identification Panel to the Antimicrobial Stewardship Program of Patients with Febrile Neutropenia in a Peruvian Referral Hospital.

The addition of Biofire® FilmArray® Blood Culture Identification panel 2 (BCID2) to the antimicrobial stewardship program (ASP) could improve outcomes in bloodstream infections (BSI) of patients with febrile neutropenia (FN). A pre- and post-quasi-experimental single-center study was conducted at a reference hospital in Peru. Three groups were considered: patients with BSI before ASP intervention (control group), patients with BSI after ASP intervention (group 1), and patients with BSI after ASP intervention plus BCID2 PCR Panel implementation (group 2). Overall, 93 patients were identified (32 control, 30 group 1, 31 group 2). The median time to effective therapy was significantly shorter in group 2 compared to group 1 and control group, respectively (3.75 vs. 10 h, p = 0.004; 3.75 vs. 19 h, p < 0.001). No significant differences in terms of relapse of bacteremia, in-hospital mortality (all cause), and 30-day-all-cause hospital readmission between the three study periods were found. The appropriateness of empirical antimicrobial use, adding or change, and the following de-escalation or discontinuation was significant when the two intervention periods were compared with the control group (p < 0.001). In addition to the lack of local studies documenting the microbiological profile of FN episodes, adding syndromic panels-based testing could allow for the consolidation of ASP strategies.

[Association between indicators of physical fitness and body weight status in high school students]. / Asociación entre indicadores de condición física, estatus de peso corporal y riesgo metabólico en escolares de secundaria.

Introduction: Introduction: the components of physical fitness have become very important to determine health problems in schoolchildren. Objective: to analyze the association between physical condition indicators (PCIs), body weight status, and metabolic risk in high school students. Methods: a cross-sectional study where 531 schoolchildren were evaluated - 271 women (51 %) and 260 men (49 %) between 11 and 15 years old. The Alpha Fitness battery was used to analyze PCIs and the Pacer test to estimate VO2max; waist circumference (WC) was measured as an indicator of obesity and metabolic risk. The students were classified according to their BMI and WC. Results: PCIs showed a poor performance in all schoolchildren in the variables of flexibility (81 %), sit-ups (97.7 %), push-ups (95.3 %), speed (99.2 %) and VO2max (64.8 %). Obese schoolchildren have significantly lower performance in flexibility, strength and speed (< 0.001) when compared to non-obese schoolchildren. On the other hand, there is a negative relationship between WC as indicator of metabolic risk and flexibility, sit-ups, and push-ups. Men are at higher metabolic risk than women. Conclusions: the relationship between HCI and obesity shows that schoolchildren with higher BMI and higher metabolic risk are those with lower performance. That is why educational policies should focus on improving the components of physical fitness to reduce obesity and metabolic risk in schoolchildren.

Introducción: Introducción: los componentes de la condición física han cobrado suma importancia para determinar problemas de salud en los escolares. Objetivo: analizar la asociación entre indicadores de condición física (ICF), estatus de peso corporal y riesgo metabólico en escolares de secundaria. Métodos: estudio de corte transversal donde se evaluaron 531 escolares, 271 mujeres (51 %) y 260 hombres (49 %) de entre 11 y 15 años. Se utilizaron la batería Alpha Fitness para analizar los ICF y la prueba Pacer para la estimación del VO2máx, y se midió la circunferencia de la cintura (CC) como indicador de obesidad y de riesgo metabólico. Los alumnos se clasificaron según su IMC y CC. Resultados: los ICF muestran un pobre desempeño en la totalidad de los escolares en las variables de flexibilidad (81 %), abdominales (97,7 %), flexión de brazos (95,3 %), velocidad (99,2 %) y VO2máx (64,8 %). Los escolares obesos presentan significativamente un menor desempeño en flexibilidad, fuerza y velocidad (< 0,001) en comparación con los escolares no obesos. Por otra parte, se presenta una relación negativa entre la CC como indicador de riesgo metabólico y la flexibilidad, los abdominales y las flexiones de brazos. Son los hombres quienes presentan mayor riesgo metabólico a diferencia de las mujeres. Conclusiones: la relación entre los ICF y la obesidad pone en evidencia que los escolares con mayor IMC y mayor riesgo metabólico son los que presentan menor desempeño. Es por ello por lo que las políticas educativas deben enfocarse en mejorar los componentes de la condición física para disminuir la obesidad y el riesgo metabólico en los escolares.

Strength and VO2max Changes by Exercise Training According to Maturation State in Children.

The health benefits of physical activity (PA) are widely recognized; however, biological maturation contributions are a subject that has been little studied, which is why the aim of this study was to analyze the effect of a six-week training program at moderate-intensity on the muscular strength and aerobic capacity in children between nine and 13 years (13 ± 1.0 years) according to their maturation state. Twenty-six schoolchildren (15 girls) participated in a six-week physical exercise program based on aerobic/anaerobic capacity and coordination skills. Maximal oxygen uptake (VO2max), trunk-lift, push-ups, curl-ups, and handgrip strength (both hands) were measured as response variables. Body mass index (BMI), skeletal maturity indicator (SMI), peak height velocity (PHV), age on peak height velocity (APHV) and sex were considered as covariates. The results of VO2max, push-ups, curl-ups, and handgrip strength were higher after the exercise program in the whole group (p < 0.05). The VO2max showed a greater increase in the normal-weight than in the overweight-obesity children (p = 0.001). Higher results in dominant handgrip strength were observed in girls (p = 0.003). The PHV before intervention presented a positive correlation with the dominant handgrip strength in all kids (r = 0.70, p = 0.001). As a conclusion, the six-week training program improved the physical fitness of children independent of the maturation state. Somatic maturation increases the physical abilities in schoolchildren.

Assessment of a small-scale fishery: Lane Snapper (Lutjanus synagris) using a length metric method.

Small-scale fisheries are hard to assess because of the limited availability of data. Therefore, a method requiring easy-to-obtain catch-data is important for the assessment and management of small-scale fisheries. The objective of this study was to assess the effect of fishing gear selectivity on a length-based metric method proposed by Froese by estimating three indicators using catch-data from Lane Snapper (Lutjanus synagris) collected in Honduras. These indicators are (1) the percentage of mature individuals in the catch, (2) the percentage of fish within the range of estimated optimal lengths to be captured, and (3) the percentage of fish larger than the optimal length. These indicators determine the level of overfishing. The indicators were estimated separately for catch-data corresponding to gill nets, and each indicator was estimated with and without selectivity correction. Selectivity and mesh sizes of the fishing gear had a major impact on the estimation of indicators 1 and 2. As for indicator 3, it consistently showed a high level of exploitation. The three estimated indicators suggested that the Lane Snapper fishery in Honduras is experiencing overfishing. Overall, the method appears to be promising for the assessment of small-scale fisheries, but it should be used cautiously.

Bacteremia due to Ralstonia mannitolilytica: A report of the first case in Peru. / Bacteremia por Ralstonia mannitolilytica: primer reporte de caso en Perú.

The first report of Ralstonia mannitolilytica bacteremia in Peru is presented. The patient was a pediatric cancer patient with a long-term central venous access device. For the diagnosis, the MicroScan Walk Away 96 automated system was used. 16S rDNA was amplified by conventional PCR, and the bacterial genus and species were identified by genetic sequencing. In addition, the bacterial resistance profile to major antimicrobials was determined. The article discusses the need to actively monitor Ralstonia mannitolilytica, especially in hospital areas of immunocompromised patients.

Se presenta el primer reporte de una bacteriemia por Ralstonia mannitolilytica en Perú. Se trata de un paciente pediátrico con cáncer que porta un dispositivo de acceso venoso central de larga duración. Para establecer el diagnóstico, se utilizó el sistema automático MicroScan Walk Away 96. Se amplificó el rADN 16S mediante PCR convencional y se identificó el género y la especie bacteriana mediante secuenciación genética. Además, se determinó el perfil de resistencia bacteriana a los principales antimicrobianos. El artículo discute la necesidad de monitorizar activamente la presencia de Ralstonia mannitolilytica, especialmente en áreas hospitalarias de pacientes inmunodeprimidos.

Correlates of sexuality in men and women aged 52-90 years attending a university medical health service in Colombia.

INTRODUCTION: Limited information is available regarding sexuality among Colombian adults aged 50 years and older. AIM: To assess demographic or health characteristics associated with sexuality measures among middle- and older-aged men and women. METHODS: Cross-sectional face-to-face interviews were conducted with retired persons from a university ambulatory medical care setting. Data on sexuality were obtained along with data on their demographic, emotional intimacy, practice of religion, medical conditions, and functional health measures. There were 136 participants. MAIN OUTCOME MEASURES: Appropriateness of sex, sexual desire, importance of sex, masturbation, and sexual intercourse. RESULTS: Fifty-seven percent of the participants were over 65 years of age, 52% were female, and 66% reported being married; 67% indicated sex is appropriate, 58% reported having sexual desire, 45% considered sex very important in their lives, 54% reported one or more instances of sexual intercourse, and 16% reported masturbating within the last year. In multivariate analyses, importance of sex and sexual intercourse decreased by age. Women had decreased odds ratios (0.20 to 0.33) for sexuality measures compared with men with the exception of appropriateness of sex. Married persons had increased odds ratios (3.06 to 9.45) for importance of sex, appropriateness of sex, and sexual intercourse compared with those of the same age who reported being unmarried. Other factors associated with some particular sexuality measures were emotional intimacy, religious practice, medical conditions, and functional health measures. There were significant mediation effects for appropriateness of sex on the relationship between sexual desire and sexual intercourse, and for importance of sex on the relationship between appropriateness of sex and sexual intercourse. CONCLUSIONS: Men and married persons had higher rates of most sexuality measures compared with women or their unmarried counterparts. These differences were greater at older ages (> or =65) for all sexuality measures except sexual intercourse.

Planetary health diet versus usual diet in adolescents. How do food and physical activity influence academic performance? / Dieta de salud planetaria contra dieta habitual en adolescentes. ¿Cómo impactan la alimentación y la actividad física al rendimiento escolar?

Objective: to analyze the relationship of the similarity of planetary health diet and habitual diet with the practice of physical activity on theacademic performance of adolescent high school students by gender.Method: 877 adolescents were evaluated. The diet is reviewed through the 24-hour reminder. Physical activity was classified according to typeof activity, frequency, duration, and intensity using the APALQ questionnaire. The caloric intake of adolescents was compared with the proposalof the EAT-LANCET commission, and the relationship between physical activity and diet with academic performance was again established.Results: Mexican adolescents do not comply with the proposal of a planetary health diet. No relationship was found between physical activityand academic performance. On the other hand, animal proteins rich in fats are consumed doubly according to the recommendation; these andwhole grains, both are inversely related to better academic performance.Conclusions: Mexican adolescents are far from consuming a planetary health diet. Physical activity was not related to academic performance, butthere is an inverse relationship between fat-rich animal proteins and whole grains in relation to subjects that contribute to academic performance,which suggests that a healthy diet such as the planetary diet proposal could have an impact on environmental sustainability and favorably impactthe academic performance of adolescents.(AU)

Objetivo: analizar la relación de la similitud entre la dieta de salud planetaria y la dieta habitual con la práctica de actividad física sobre elrendimiento académico en adolescentes estudiantes de secundaria por género.Método: se evaluaron 877 adolescentes. La dieta se revisó a través del recordatorio de 24 horas. Se clasificó la actividad física según el tipo deactividad, frecuencia, duración e intensidad con el cuestionario APALQ. Se comparó la ingesta calórica de los adolescentes con la propuesta porla comisión EAT-LANCET y se establecieron relaciones entre la actividad física y la dieta con el rendimiento académico.Resultados: los adolescentes mexicanos no cumplen con la propuesta de una dieta de salud planetaria. No se encontró relación entre laactividad física y el rendimiento académico. Por otro lado, las proteínas animales ricas en grasas se consumen doblemente de acuerdo con larecomendación; estas y los cereales integrales, ambos se relacionan inversamente con un mejor rendimiento académico.Conclusiones: los adolescentes mexicanos están lejos de consumir una dieta planetaria saludable. La actividad física no se relacionó con elrendimiento académico, pero sí existe una relación inversa entre las proteínas animales ricas en grasas y los cereales integrales con relación amaterias que contribuyen con el rendimiento académico, lo que sugiere que una alimentación saludable como la propuesta de la dieta planetariapodría tener impacto sobre la sostenibilidad ambiental e impactar favorablemente en el rendimiento académico de los adolescente.(AU)

[Costs of Chagas' disease screening test in blood donors in two Colombian blood banks, 2015].

INTRODUCTION: Transfusion is a mechanism of transmission of Chagas' disease. There are no studies on the costs of the screening test in Colombian blood banks. OBJECTIVE: To estimate the costs of the screening test for Chagas' disease among blood donors in two Colombian blood banks, 2015. MATERIALS AND METHODS: We conducted a micro-costing study from the perspective of the health care provider to estimate the cost of Chagas' disease testing in two blood banks, Banco de Sangre de la Cruz Roja, Seccional Bolívar, and Banco de Sangre del Hospital de Yopal, Casanare, taking into account four cost categories: 1) Administrative costs: public services and insurance costs were calculated based on the blood bank area in square meters; 2) capital costs: building and equipment costs that were annualized using a 3% discount rate and a lifespan of 20 years for building and five for equipment; 3) costs of Chagas' disease test materials and reagents adjusted by blood bank production level, and 4) costs of staff in charge of Chagas' disease test processing. The costs of transfusion bagsand immunohematology tests are also reported. RESULTS: The cost of Chagas' disease test in the blood bank of Seccional Bolívar was COP$ 37,804 (USD$ 12), and the blood bag and immunohematology test costs were COP$ 25,941 (USD$ 8.2) and COP$ 6,800 (USD$ 2.2), respectively. In the blood bank of Yopal, Casanare, the costs were COP$ 77,384 (USD$ 24.6), COP$ 30,141 (USD$ 9.6) and COP$ 12,627 (USD$ 4), respectively. Personnel cost accounted for the highest percentage of the total cost for both blood banks (47.5% in Seccional Bolívar, and 55.7% in Yopal, Casanare). CONCLUSION: Our results are an important input for the planning of services and cost-effectiveness studies for screening tests for Chagas' disease in Colombian blood banks.

Inequalities on mortality due to acute respiratory infection in children: A Colombian analysis. / Desigualdades en la mortalidad por infección respiratoria aguda en niños: un análisis colombiano

INTRODUCTION: Acute respiratory infections (ARI) are a leading public health issue worldwide. OBJECTIVE: To explore the inequalities in ARI mortality rates in under-5, according to socioeconomic characteristics. MATERIALS AND METHODS: We conducted an ecological analysis to study inequalities at municipal level due to ARI mortality in children under 5 years. The data were obtained from official death records of the Departamento Administrativo Nacional de Estadística. The analysis of inequalities in the under-5 mortality rate (U5MR) included: 1) Classification of the population in different socio-economic strata, and 2) measurement of the degree of inequality. We used the ARI-U5MR as an outcome measurement.The mortality rates were estimated at national and municipal levels for the years 2000, 2005, 2010, and 2013. Rate ratios, rates differences, and concentration curves were calculated to observe the inequalities. RESULTS: A total of 18,012 children under 5 years died by ARI in Colombia from 2000 to 2013. ARIU5MR was greater in boys than in girls. During this period, an increase in the infant mortality relative gap in both boys and girls was observed. In 2013, the U5MR evidenced that for boys from municipalities with the highest poverty had a 1.6-fold risk to die than those in municipalities with the lowest poverty (low tercile). In girls, the ARI-U5MR for 2005 and 2013 in the poorest tercile was 1.5 and 2 times greater than in the first tercile, respectively. CONCLUSION: Colombian inequalities in the ARI mortality rate among the poorest municipalities compared to the richest ones continue to be a major challenge in public health.

Introducción. Las infecciones respiratorias agudas (IRA) son un importante problema de salud pública a nivel mundial. Objetivo. Explorar las desigualdades de la tasa de mortalidad debida a IRA (TM-IRA) en <5 años, de acuerdo a variables socioeconómicas. Materiales y métodos. Se realizó un análisis ecológico para estudiar las desigualdades a nivel municipal de las TM-IRA en <5 años. Los datos se obtuvieron a partir de registros de muertes del Departamento Administrativo Nacional de Estadística. En análisis de desigualdades en <5 incluyó: 1) Clasificación de la población por estatus socioeconómico y 2) Medición del grado de desigualdad. Como resultado en salud se utilizó la TM-IRA en <5 años. Se estimaron tasas a nivel nacional y municipal para 2000, 2005, 2010, 2013. Se calcularon razones y diferencias de tasas y curvas de concentración para observar las desigualdades. Resultados. Entre 2000-2013 murieron en Colombia por IRA 18.012 <5 años. La TM-ARI fue mayor en niños que en niñas. En el periodo, se observó un incremento en la brecha de mortalidad infantil en ambos sexos. En 2013, la tasa en niños que murieron en municipios con mayor pobreza fue 1,6 veces mayor que la de niños en aquellos con menor. En niñas, para 2015 y 2013, la tasa en el tercil más pobre fue 1,5 y 2 veces mayor que la del primer tercil, respectivamente. Conclusión. Las desigualdades en la TM-IRA entre los municipios más pobres en comparación con los más ricos continúan siendo un reto importante en salud pública.

Metastatic Castration-Resistant Prostate Cancer Previously Treated With Docetaxel-Based Chemotherapy: Treatment Patterns From the PROXIMA Prospective Registry.

PURPOSE: There is a major clinical need to devise an optimal treatment sequence for the multiple therapy options available for patients with metastatic castration-resistant prostate cancer (mCRPC). In the absence of prospective clinical trials, sequencing information can be derived from large, real-world registry studies. PATIENTS AND METHODS: PROXIMA (Treatment Patterns in Patients With Metastatic Castration-Resistant Prostate Cancer Previously Treated With Docetaxel-Based Chemotherapy) is a large, global, prospective registry study evaluating real-world treatment patterns of patients with mCRPC who experience disease progression during or after docetaxel therapy. Patients were enrolled worldwide between 2011 and 2014. Treatments were determined by the treating physicians and recorded in categories of chemotherapy, hormonal therapy, targeted therapy, immunotherapy, and palliative therapy. Treatment sequencing patterns, response to treatment, and types of progression were recorded and analyzed. Progression-free survival and overall survival with different treatment modalities were analyzed using Kaplan-Meier method. RESULTS: Treatment patterns were evaluated in 903 patients. Therapy selection was influenced by region. Hormonal therapy (57.5%) and taxane chemotherapy (26.4%) were the most frequently administered first subsequent treatments after docetaxel. Tumor responses to first subsequent treatment were observed in 22.6% of evaluable patients. Overall survival and progression-free survival did not differ significantly across different treatment modalities. CONCLUSION: Identifying an optimal treatment sequence is vital for improving the care of patients with mCRPC. The PROXIMA registry provided a representative sample of global data on real-world treatment patterns for patients with mCRPC previously treated with docetaxel. These data can be used to devise optimal therapy sequences and inform treatment decisions.

Dermatoglyphic Profiles of Competitive Athletes: CrossFit and Ultra-Marathon / Perfiles Dermatoglíficos de Deportistas Competitivos: CrossFit y Ultramaratón

SUMMARY: In the sports field, sports are usually classified according to the predominance of the energy system used during the competition; sports like Crossfit and ultra-marathon could be totally opposite due to the needs of athletes to achieve good places. Due to the above, the objective was established to compare the dermatoglyphic profiles of CrossFit and Ultra-marathon athletes according to the predisposition of the energy systems, aerobic and anaerobic, used in the competitions. Cross-sectional descriptive study, where the dermatoglyphic profile of 21 athletes of national presence (10 crossfit and 11 ultra-marathon runners) was determined, through the Computerized Dermatoglyphic System, brand Salus Dermatoglifia, according to the Cummins and Midlo protocol, which consists of taking the fingerprints of the 10 fingers of the hands. The fingerprint designs of the human being (arch, loop and whorl) and the elements contained in them (nucleus and delta) were analyzed, differences were established through the Analysis of Variance test in the SPSS V.25 statistical package. The results show significant differences between the average scores of the line count of both disciplines (108 and 165), being higher in ultra-marathon runners, associated with greater aerobic resistance. Another significant and relevant finding was the presence of arch-type fingerprints only in CrossFit athletes associated, along with low line count, with strength and power. It is concluded that Crossfit and Ultra-marathon athletes have dissimilar natural physical characteristics, which is why they participate and excel in different sports where they have enhanced their natural physical abilities through training.

En el ámbito deportivo, los deportes suelen clasificarse según el predominio del sistema energético utilizado durante la competición; Deportes como el Crossfit y la ultramaratón podrían ser totalmente opuestos debido a las necesidades de los deportistas de conseguir buenos lugares. Debido a lo anterior, se estableció como objetivo comparar los perfiles dermatoglíficos de atletas de CrossFit y Ultramaratón según la predisposición de los sistemas energéticos, aeróbico y anaeróbico, utilizados en las competencias. Estudio descriptivo transversal, donde se determinó el perfil dermatoglífico de 21 deportistas de presencia nacional (10 crossfit y 11 ultramaratonistas), a través del Sistema Dermatoglífico Computarizado, marca Salus Dermatoglifia, según el protocolo Cummins y Midlo, el cual consta de tomando las huellas dactilares de los 10 dedos de las manos. Se analizaron los diseños dactilares del ser humano (arco, asa y verticilo) y los elementos contenidos en ellos (núcleo y delta), se establecieron diferencias mediante la prueba de Análisis de Varianza en el paquete estadístico SPSS V.25. Los resultados muestran diferencias significativas entre las puntuaciones medias del recuento de líneas de ambas disciplinas (108 y 165), siendo superiores en los corredores de ultramaratón, asociado a una mayor resistencia aeróbica. Otro hallazgo significativo y relevante fue la presencia de huellas dactilares tipo arco sólo en atletas de CrossFit asociadas, junto con un bajo número de líneas, con fuerza y potencia. Se concluye que los atletas de Crossfit y Ultramaratón tienen características físicas naturales disímiles, por lo que participan y destacan en diferentes deportes donde han potenciado sus capacidades físicas naturales a través del entrenamiento.