RESUMEN
The sympathetic nervous system modulates the growth of C-1300 mouse neuroblastoma in vivo and in vitro. We now report that a mitogenic/trophic factor that augments growth of C-1300 neuroblastoma and of the S-20 neuroblastoma clonal line is present in freshly excised sympathetic cervical ganglia from newborn rats, but is not detectable in homogenates from sympathetic ganglia obtained from adult rats.
Asunto(s)
Neuroblastoma/patología , Sistema Nervioso Simpático/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Células Cultivadas , Sustancias de Crecimiento/fisiología , RatasRESUMEN
The sympathetic nervous system exerts a trophic-mitogenic influence on C-1300 mouse neuroblastoma. We now report that sympathetic axotomy suppresses growth of the S-20 clonal line of neuroblastoma but does not influence the growth in vivo of two other clonal lines, NIE-115 and C-46. Sympathetic ganglia-conditioned medium significantly increases proliferation of S-20 cells in vitro. Growth of NIE-115 and C-46 clonal neuroblastoma lines is not influenced by sympathetic ganglia-conditioned medium. We postulate that the sympathetic nervous system secretes a mitogenic-trophic factor that favors growth of C-1300 neuroblastoma in vivo. Sensitivity to this factor varies between neuroblastoma clonal lines.
Asunto(s)
Neuroblastoma/patología , Sistema Nervioso Simpático/fisiología , Animales , División Celular , Células Cultivadas , Medios de Cultivo , Ganglios Simpáticos/fisiología , Ratones , RatasRESUMEN
A trophic factor secreted by PC12 rat pheochromocytoma augments growth of C1300 neuroblastoma clonal lines S20, N18 and C46, but does not affect growth of the NIE 115 line. A trophic factor present in newborn sympathetic ganglia has the same biological effect on neuroblastoma cell lines. PC12 cells and sympathetic ganglia are both of neural crest origin; possibly both secrete the same trophic factor.
Asunto(s)
Ganglios Simpáticos/metabolismo , Factores de Crecimiento Nervioso/fisiología , Proteínas del Tejido Nervioso/fisiología , Neuroblastoma/patología , Feocromocitoma/metabolismo , Neoplasias de las Glándulas Suprarrenales/metabolismo , Animales , Ratones , Ratas , Ratas Endogámicas WF , Células Tumorales Cultivadas/metabolismoRESUMEN
We have reported previously that ablation of the sympathetic nervous system augments immune responses in mice and rats. In the present study we show that a factor present in the sympathetic cervical ganglia of newborn rats suppresses Con A-induced stimulation of splenic T lymphocytes significantly whether added prior to, throughout, or following exposure to Con A. We also show that rat PC 12 pheochromocytoma cells secrete a factor which has the same inhibitory effect on T cell proliferation as the sympathetic ganglia-derived factor.
Asunto(s)
Medios de Cultivo/farmacología , Ganglios Simpáticos/inmunología , Activación de Linfocitos/inmunología , Linfocitos T/efectos de los fármacos , Células Tumorales Cultivadas/inmunología , Animales , Concanavalina A/farmacología , Técnicas In Vitro , Ratas , Ratas Endogámicas , Bazo/inmunologíaRESUMEN
C-6 glioma cells possess beta-adrenergic receptors on the cell surface. Activation of beta-adrenergic receptors with beta-adrenergic agonists increases intracellular levels of cAMP and leads to differentiation of C-6 glioma cells in vitro. The present study shows that growth of C-6 glioma tumor in rats with ablated sympathetic nervous system is augmented as compared to controls. Lack of normal noradrenergic stimulation of C-6 glioma cells may lower intracellular cAMP and allow unrestricted growth of this tumor.