RESUMEN
Aberrant DNA methylation of genes is closely linked to many aspects of tumor development. This study focuses on the effect of DNA hypermethylation of von Willebrand factor C domain containing 2 (VWC2) on colorectal cancer (CRC) progression and the underpinning mechanism. According to data in the bioinformatic systems, VWC2 had the highest degree of DNA methylation in colonic adenocarcinoma, and it showed DNA hypermethylation in rectal adenocarcinoma as well. CRC and the para-tumorous tissues were collected from 86 patients. VWC2 was expressed at low levels in CRC samples and inversely correlated with tumor stage and tumor biomarker expression. DNA hypermethylation and reduced expression of VWC2 were also detected in CRC cell lines HCT-116 and HT29. VWC2 overexpression suppressed the malignant growth of cells in vitro and in vivo. Co-immunoprecipitation and western blot assays showed that small ubiquitin-like modifier 1 (SUMO1) mediated SUMOylation of DNA methyltransferase 1 (DNMT1) and strengthened its protein stability, which promoted DNA methylation and suppression of the VWC2 gene. In summary, this study demonstrates that SUMO1-mediated activation of DNMT1 induces DNA methylation and downregulation of VWC2 in CRC to augment cancer development.
Asunto(s)
Adenocarcinoma , Neoplasias Colorrectales , Humanos , Metilación de ADN , Neoplasias Colorrectales/patología , ADN , Metiltransferasas/genética , Adenocarcinoma/genética , Regulación Neoplásica de la Expresión Génica , Proteína SUMO-1/genética , Proteína SUMO-1/metabolismoRESUMEN
<p><b>OBJECTIVE</b>To probe and systematize the ancient cauterization with pyropuncture needle for evacuation of pus.</p><p><b>METHODS</b>One hundred and eight cases of body surface abscess were randomly divided into 2 groups. The cauterization group (n=84) were treated by drainage with electric cauterization and the incision group (n=24) by drainage with incision. Clinical therapeutic effect, the healing time and the scar area of the wound were compared between the two groups.</p><p><b>RESULTS</b>All of the patients in the two groups were effective with a similar therapeutic effect (P > 0.05); the cauterization group in the healing time and the scar area of the wound was superior to the incision group (P < 0.05).</p><p><b>CONCLUSION</b>Electric cauterization for evacuation of pus has advantages of rapid healing and small scar area of wound.</p>