RESUMEN
We describe the isolation of a Francisella sp. from normally sterile sites in acutely ill patients in two different states within 2 years. Microbiologic and molecular analyses indicate that this organism represents a novel Francisella sp. Clinicians and microbiologists should be aware of this new potential pathogen, as infection may be more common than recognized.
Asunto(s)
Sangre/microbiología , Líquido Cefalorraquídeo/microbiología , Francisella/clasificación , Francisella/aislamiento & purificación , Anciano de 80 o más Años , Pruebas de Aglutinación , Bacteriemia/microbiología , Técnicas de Tipificación Bacteriana , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Femenino , Francisella/genética , Humanos , Lactante , Masculino , Meningoencefalitis/microbiología , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , Homología de Secuencia de Ácido NucleicoRESUMEN
In this paper we evaluate how to effectively use the crowdsourcing service, Amazon's Mechanical Turk (MTurk), to content analyze textual data for use in psychological research. MTurk is a marketplace for discrete tasks completed by workers, typically for small amounts of money. MTurk has been used to aid psychological research in general, and content analysis in particular. In the current study, MTurk workers content analyzed personally-written textual data using coding categories previously developed and validated in psychological research. These codes were evaluated for reliability, accuracy, completion time, and cost. Results indicate that MTurk workers categorized textual data with comparable reliability and accuracy to both previously published studies and expert raters. Further, the coding tasks were performed quickly and cheaply. These data suggest that crowdsourced content analysis can help advance psychological research.