Effect of addition of melatonin on liquid storage of ram semen at 4°C.

Adding a certain amount of antioxidants to semen extender has been shown to improve semen quality. The aim of present study was to elucidate whether the supplementation of melatonin to the Tris-based extender (CTR) could enhance the quality of ram spermatozoa during storage at 4°C. Ram semen samples were collected and diluted with CTR extender containing different concentrations (0, 0.05 (M 0.05), 0.1 (M 0.1), 0.2 (M 0.2) or 0.4 (M 0.4) mM) of melatonin. Sperm routine indicators, mitochondrial activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were analysed in control and melatonin treatment groups. The higher per cent of motility, plasma membrane integrity, mitochondrial activity and T-AOC activity was observed in M 0.05, M 0.1 and M 0.2 groups compared to control group at 5 days of storage (p < 0.05), while lower percentage of MDA content was observed among these groups (p < 0.05). In addition, there were no significant differences in acrosome integrity among the control and M 0.05, M 0.1 and M 0.2 groups during the experiment. The above results show that the addition of 0.05, 0.1, 0.2 mM of melatonin is beneficial to the preservation of ram semen during liquid storage at 4°C mainly through antioxidative stress.

Lycopene and alpha-lipoic acid improve semen antioxidant enzymes activity and cashmere goat sperm function after cryopreservation.

Cashmere goats, a unique biological resource in China, have the highest cashmere yield and best fiber quality in the world. The goal of this study was to determine the effects of cryopreserving with lycopene (LP) and alpha-lipoic acid (ALA) on the physiological characteristics of Cashmere goat spermatozoa. The results showed that sperm motility, acrosome integrity, membrane integrity, and mitochondrial activity of goat spermatozoa were greater in extenders containing 1.0 mg/mL LP and 10 µg/mL ALA (P < 0.05). Furthermore, higher SOD, CAT, and GSH-Px levels in semen occurred for extenders with 1.0 mg/mL LP and 10 µg/mL ALA compared with that of other treatments and the control group (P < 0.05). Interestingly, this study also combined LP + ALA in the extender. The results showed that the sperm motility, membrane integrity, and acrosome integrity in 1.0 mg/mL + 5 µg/mL in the LP + ALA group were significantly elevated compared with that of the control group (P < 0.05). Moreover, 1.0 mg/mL + 5 µg/mL LP + ALA addition increased SOD, CAT, and GSH-Px in spermatozoa more than 1.0 mg/mL LP and 10 µg/mL ALA (P < 0.05). In addition, the results of AI showed that the pregnancy rates were higher in the 1.0 mg/mL + 5 µg/mL LP + ALA group than the control group (P < 0.05). In conclusion, the addition of LP + ALA to extender solutions protects goat spermatozoa from ROS attack by improving antioxidant enzymes activity, and the results suggested that freezing extenders supplemented with 1.0 mg/mL + 5 µg/mL LP + ALA would be beneficial to the Cashmere goat breeding industry.

Low-opportunity-cost feed can reduce land-use-related environmental impacts by about one-third in China.

Feeding animals more low-opportunity-cost feed products (LCFs), such as food waste and by-products, may decrease food-feed competition for cropland. Using a feed allocation optimization model that considers the availability of feed sources and animal requirements for protein and energy, we explored the perspectives of feeding more LCFs to animals in China. We found that about one-third of the animal feed consisted of human-edible products, while only 23% of the available LCFs were used as feed during 2009-2013. An increased utilization of LCFs (45-90 Mt) could potentially save 25-32% of feed-producing cropland area without impairing livestock productivity. Parallelly, about one-third of feed-related irrigation water, synthetic fertilizer and greenhouse gas emissions would be saved. Re-allocating the saved cropland could sustain the food energy demand of 30-185 million people. Achieving the potentials of increased LCF use requires improved technology and coordination among stakeholders.

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C.

Sulfanilamide (SA) is an effective broad-spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.